spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roseman, R. R.
Right arrow Articles by Geyer, P. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roseman, R. R.
Right arrow Articles by Geyer, P. K.

Development, Vol 121, Issue 11 3573-3582, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

A Drosophila insulator protein facilitates dosage compensation of the X chromosome min-white gene located at autosomal insertion sites

RR Roseman, JM Swan and PK Geyer
Department of Biochemistry, University of Iowa, College of Medicine, Iowa City 52242, USA.

The suppressor of Hairy-wing [su(Hw)] gene encodes a zinc finger protein that binds to a repeated motif in the gypsy retrotransposon. These DNA sequences, called the su(Hw)-binding region, have properties of an insulator region because they (1) disrupt enhancer/silencer function in a position-dependent manner and (2) protect the mini-white gene from both euchromatic and heterochromatic position effects. To gain further insights into the types of position effects that can be insulated, we determined the effects of the su(Hw)-binding region on dosage compensation of the X-linked mini-white gene. Dosage compensation is the process that equalizes the unequal content of X-linked genes in males and females by increasing the X-linked transcription level twofold in males. Transposition of X-linked genes to the autosomes commonly results in incomplete dosage compensation, indicating that the distinct male X chromatin environment is important for this process. We found that dosage compensation of autosomally integrated mini-white genes flanked by su(Hw)-binding regions was greatly improved, such that complete or nearly complete compensation was observed at the majority of insertion sites. The su(Hw) protein was essential for this enhanced dosage compensation because in a su(Hw) mutant background compensation was incomplete. These experiments provide evidence that the su(Hw)-binding region facilitates dosage compensation of the mini-white gene on the autosomes. This may result from protection of the mini-white gene from a negative autosomal chromatin environment.


This article has been cited by other articles:


Home page
 DevelopmentHome page
K. J. T. Venken and H. J. Bellen
Transgenesis upgrades for Drosophila melanogaster
Development, October 15, 2007; 134(20): 3571 - 3584.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Chen and V. G. Corces
The Gypsy Insulator of Drosophila Affects Chromatin Structure in a Directional Manner
Genetics, December 1, 2001; 159(4): 1649 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. R. Roseman, K. Morgan, D. R. Mallin, R. Roberson, T. J. Parnell, D. J. Bornemann, J. A. Simon, and P. K. Geyer
Long-Range Repression by Multiple Polycomb Group (PcG) Proteins Targeted by Fusion to a Defined DNA-Binding Domain in Drosophila
Genetics, May 1, 2001; 158(1): 291 - 307.
[Abstract] [Full Text]

Home page
 GeneticsHome page
U. Bhadra, M. Pal-Bhadra, and J. A. Birchler
Role of the male specific lethal (msl) Genes in Modifying the Effects of Sex Chromosomal Dosage in Drosophila
Genetics, May 1, 1999; 152(1): 249 - 268.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1995