|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 12 4103-4115, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
J Sechrist, MA Nieto, RT Zamanian and M Bronner-Fraser
Developmental Biology Center, University of California, Irvine 92717, USA.
After unilateral ablation of the avian cranial neural folds, the remaining neuroepithelial cells are able to replace the missing neural crest population (Scherson et al., 1993). Here, we characterize the cellular and molecular nature of this regulative response by defining: (1) the time and location of neural crest cell production by the neuroepithelium; (2) rostrocaudal axial differences in the regulative response; and (3) the onset of expression of Slug, a transcription factor present in premigratory and migrating neural crest cells. Using DiI and HNK-1 antibody labeling techniques, we find that neural crest regeneration occurs only after apposition of the remaining neuroepithelium with the epidermis, suggesting that the developmental mechanism underlying regeneration of the neural crest may recapitulate initial generation of the neural crest. The regulative response occurs maximally at the 3-5 somite stage, and slowly declines thereafter. Surprisingly, there are profound regional differences in the regenerative ability. Whereas a robust regulation occurs in the caudal midbrain/hindbrain, the caudal forebrain/rostral midbrain regenerates neural crest to a much lesser extent. After neural fold removal in the hindbrain, regenerated neural crest cells migrate in a segmental pattern analogous to that seen in unablated embryos; a decrease in regulative response appears to occur with increasing depth of the ablation. Up-regulation of Slug appears to be an early response after ablation, with Slug transcripts detectable proximal to the ablated region 5-8 hours after surgery and prior to emergence of neural crest cells. Both bilateral and unilateral ablations yield substantial numbers of neural crest cells, though the former recover less rapidly and have greater deficits in neural crest-derived structures than the latter. These experiments demonstrate that the regulative ability of the cranial neuroepithelium to form neural crest depends on the time, location and extent of neural fold ablation.
This article has been cited by other articles:
|
|
 |
|
  K. L. McCabe and M. Bronner-Fraser Essential role for PDGF signaling in ophthalmic trigeminal placode induction Development, May 15, 2008; 135(10): 1863 - 1874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Andoniadou, M. Signore, E. Sajedi, C. Gaston-Massuet, D. Kelberman, A. J. Burns, N. Itasaki, M. Dattani, and J. P. Martinez-Barbera Lack of the murine homeobox gene Hesx1 leads to a posterior transformation of the anterior forebrain Development, April 15, 2007; 134(8): 1499 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xu, R. Francis, C. J. Wei, K. L. Linask, and C. W. Lo Connexin 43-mediated modulation of polarized cell movement and the directional migration of cardiac neural crest cells Development, September 15, 2006; 133(18): 3629 - 3639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Taneyhill and M. Bronner-Fraser Dynamic Alterations in Gene Expression after Wnt-mediated Induction of Avian Neural Crest Mol. Biol. Cell, November 1, 2005; 16(11): 5283 - 5293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Kulesa, M Bronner-Fraser, and S Fraser In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest Development, January 7, 2000; 127(13): 2843 - 2852. [Abstract] [PDF]
|
|
|
|
|
|
C. Baker and M Bronner-Fraser Establishing neuronal identity in vertebrate neurogenic placodes Development, January 7, 2000; 127(14): 3045 - 3056. [Abstract] [PDF]
|
|
|
|
|
|
H Epperlein, D Meulemans, M Bronner-Fraser, H Steinbeisser, and M. Selleck Analysis of cranial neural crest migratory pathways in axolotl using cell markers and transplantation Development, January 6, 2000; 127(12): 2751 - 2761. [Abstract] [PDF]
|
|
|
|
|
|
C. Baker, M. Stark, C Marcelle, and M Bronner-Fraser Competence, specification and induction of Pax-3 in the trigeminal placode Development, January 1, 1999; 126(1): 147 - 156. [Abstract] [PDF]
|
|
|
|
 |
|
 B. J. Martinsen and M. Bronner-Fraser Neural Crest Specification Regulated by the Helix-Loop-Helix Repressor Id2 Science, August 14, 1998; 281(5379): 988 - 991. [Abstract] [Full Text]
|
|
|
|
|
|
M. Stark, J Sechrist, M Bronner-Fraser, and C Marcelle Neural tube-ectoderm interactions are required for trigeminal placode formation Development, January 11, 1997; 124(21): 4287 - 4295. [Abstract] [PDF]
|
|
|
|
|
|
J. Saldivar, J. Sechrist, C. Krull, S Ruffins, and M Bronner-Fraser Dorsal hindbrain ablation results in rerouting of neural crest migration and changes in gene expression, but normal hyoid development Development, January 7, 1997; 124(14): 2729 - 2739. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Frank Restriction in Cell Fates of Developing Spinal Cord Cells Transplanted to Neural Crest Pathways J. Neurosci., December 1, 1996; 16(23): 7638 - 7648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Couly, A Grapin-Botton, P Coltey, and N. Le Douarin The regeneration of the cephalic neural crest, a problem revisited: the regenerating cells originate from the contralateral or from the anterior and posterior neural fold Development, January 11, 1996; 122(11): 3393 - 3407. [Abstract] [PDF]
|
|
|
|
|
|
N. Holland, G Panganiban, E. Henyey, and L. Holland Sequence and developmental expression of AmphiDll, an amphioxus Distal-less gene transcribed in the ectoderm, epidermis and nervous system: insights into evolution of craniate forebrain and neural crest Development, January 9, 1996; 122(9): 2911 - 2920. [Abstract] [PDF]
|
|
