|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 2 347-357, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
MA Akimenko, SL Johnson, M Westerfield and M Ekker
Loeb Institute for Medical Research, Ottawa Civic Hospital, Ontario, Canada.
To study the genetic regulation of growth control and pattern formation during fin development and regeneration, we have analysed the expression of four homeobox genes, msxA, msxB, msxC and msxD in zebrafish fins. The median fin fold, which gives rise to the unpaired fins, expresses these four msx genes during development. Transcripts of the genes are also present in cells of the presumptive pectoral fin buds. The most distal cells, the apical ectodermal ridge of the paired fins and the cleft and flanking cells of the median fin fold express all these msx genes with the exception of msxC. Mesenchymal cells underlying the most distal cells express all four genes. Expression of the msx genes in the fin fold and fin buds is transient and, by 3 days after fertilization, msx expression in the median fin fold falls below levels detectable by in situ hybridization. Although the fins of adult zebrafish normally have levels of msx transcripts undetectable by in situ hybridization, expression of all four genes is strongly reinduced during regeneration of both paired and unpaired fins. Induction of msx gene expression in regenerating caudal fins occurs as early as 30 hours postamputation. As the blastema forms, the levels of expression increase and reach a maximum between the third and fifth days. Then, msx expression progressively declines and disappears by day 12 when the caudal fin has grown back to its normal size. In the regenerating fin, the blastema cells that develop at the tip of each fin ray express msxB and msxC. Cells of the overlying epithelium express msxA and msxD, but do not express msxB or msxC. Amputations at various levels along the proximodistal axis of the fin suggest that msxB expression depends upon the position of the blastema, with cells of the rapidly proliferating proximal blastema expressing higher levels than the cells of the less rapidly proliferating distal blastema. Expression of msxC and msxD is independent of the position of the blastema cell along this axis. Our results suggest distinct roles for each of the four msx genes during fin development and regeneration and differential regulation of their expression.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. A. Wills, A. R. Kidd III, A. Lepilina, and K. D. Poss Fgfs control homeostatic regeneration in adult zebrafish fins Development, September 15, 2008; 135(18): 3063 - 3070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Tayeh, H.-J. Yen, J. S. Beck, C. C. Searby, T. A. Westfall, H. Griesbach, V. C. Sheffield, and D. C. Slusarski Genetic interaction between Bardet-Biedl syndrome genes and implications for limb patterning Hum. Mol. Genet., July 1, 2008; 17(13): 1956 - 1967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Stoick-Cooper, R. T. Moon, and G. Weidinger Advances in signaling in vertebrate regeneration as a prelude to regenerative medicine Genes & Dev., June 1, 2007; 21(11): 1292 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Stoick-Cooper, G. Weidinger, K. J. Riehle, C. Hubbert, M. B. Major, N. Fausto, and R. T. Moon Distinct Wnt signaling pathways have opposing roles in appendage regeneration Development, February 1, 2007; 134(3): 479 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. J. Pyati, M. S. Cooper, A. J. Davidson, A. Nechiporuk, and D. Kimelman Sustained Bmp signaling is essential for cloaca development in zebrafish Development, June 1, 2006; 133(11): 2275 - 2284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Mathew, E. A. Andreasen, and R. L. Tanguay Aryl Hydrocarbon Receptor Activation Inhibits Regenerative Growth Mol. Pharmacol., January 1, 2006; 69(1): 257 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Whitehead, S. Makino, C.-L. Lien, and M. T. Keating fgf20 Is Essential for Initiating Zebrafish Fin Regeneration Science, December 23, 2005; 310(5756): 1957 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Flanagan-Steet, M. A. Fox, D. Meyer, and J. R. Sanes Neuromuscular synapses can form in vivo by incorporation of initially aneural postsynaptic specializations Development, October 15, 2005; 132(20): 4471 - 4481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Makino, G. G. Whitehead, C.-L. Lien, S. Kim, P. Jhawar, A. Kono, Y. Kawata, and M. T. Keating Heat-shock protein 60 is required for blastema formation and maintenance during regeneration PNAS, October 11, 2005; 102(41): 14599 - 14604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakaniwa, M. Hirayama, A. Shimizu, T. Sasaki, S. Asakawa, N. Shimizu, and S. Watabe Genomic sequences encoding two types of medaka hemopexin-like protein Wap65, and their gene expression profiles in embryos J. Exp. Biol., May 15, 2005; 208(10): 1915 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Thorpe, G. Weidinger, and R. T. Moon Wnt/{beta}-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish Development, April 15, 2005; 132(8): 1763 - 1772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Zodrow and R. L. Tanguay 2,3,7,8-Tetrachlorodibenzo-p-dioxin Inhibits Zebrafish Caudal Fin Regeneration Toxicol. Sci., November 1, 2003; 76(1): 151 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fischer, B. W. Draper, and C. J. Neumann The zebrafish fgf24 mutant identifies an additional level of Fgf signaling involved in vertebrate forelimb initiation Development, August 1, 2003; 130(15): 3515 - 3524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Poss, A. Nechiporuk, A. M. Hillam, S. L. Johnson, and M. T. Keating Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration Development, March 13, 2003; 129(22): 5141 - 5149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Quint, A. Smith, F. Avaron, L. Laforest, J. Miles, W. Gaffield, and M.-A. Akimenko From the Cover: Bone patterning is altered in the regenerating zebrafish caudal fin after ectopic expression of sonic hedgehog and bmp2b or exposure to cyclopamine PNAS, June 25, 2002; 99(13): 8713 - 8718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nechiporuk and M. T. Keating A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration Development, January 6, 2002; 129(11): 2607 - 2617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mintzer, M. Lee, G Runke, J Trout, M Whitman, and M. Mullins Lost-a-fin encodes a type I BMP receptor, Alk8, acting maternally and zygotically in dorsoventral pattern formation Development, January 3, 2001; 128(6): 859 - 869. [Abstract] [PDF]
|
|
|
|
|
|
R. Cornell and J. Eisen Delta signaling mediates segregation of neural crest and spinal sensory neurons from zebrafish lateral neural plate Development, January 7, 2000; 127(13): 2873 - 2882. [Abstract] [PDF]
|
|
|
|
|
|
V. Nguyen, J Trout, S. Connors, P Andermann, E Weinberg, and M. Mullins Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway Development, January 3, 2000; 127(6): 1209 - 1220. [Abstract] [PDF]
|
|
|
|
 |
|
 A. J. Davidson, J. H. Postlethwait, Y.-L. Yan, D. R. Beier, C. van Doren, D. Foernzler, A. J. Celeste, K. E. Crosier, and P. S. Crosier Isolation of Zebrafish gdf7 and Comparative Genetic Mapping of Genes Belonging to the Growth/Differentiation Factor 5, 6, 7 Subgroup of the TGF-beta Superfamily Genome Res., February 1, 1999; 9(2): 121 - 129. [Abstract] [Full Text]
|
|
|
|
|
|
C. Neumann, H Grandel, W Gaffield, S Schulte-Merker, and C Nusslein-Volhard Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity Development, January 11, 1999; 126(21): 4817 - 4826. [Abstract] [PDF]
|
|
|
|
|
|
S. Connors, J Trout, M Ekker, and M. Mullins The role of tolloid/mini fin in dorsoventral pattern formation of the zebrafish embryo Development, January 6, 1999; 126(14): 3119 - 3130. [Abstract] [PDF]
|
|
|
|
|
|
L Laforest, C. Brown, G Poleo, J Geraudie, M Tada, M Ekker, and M. Akimenko Involvement of the sonic hedgehog, patched 1 and bmp2 genes in patterning of the zebrafish dermal fin rays Development, January 11, 1998; 125(21): 4175 - 4184. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Takahashi, C. Guron, S. Shetty, H. Matsui, and R. Raghow A Minimal Murine Msx-1 Gene Promoter. ORGANIZATION OF ITS cis-REGULATORY MOTIFS AND THEIR ROLE IN TRANSCRIPTIONAL ACTIVATION IN CELLS IN CULTURE AND IN TRANSGENIC MICE J. Biol. Chem., September 5, 1997; 272(36): 22667 - 22678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rifas, D. A. Towler, and L. V. Avioli Gestational exposure to ethanol suppresses msx2 expression in developing mouse embryos PNAS, July 8, 1997; 94(14): 7549 - 7554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Maas and M. Bei The Genetic Control of Early Tooth Development Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(1): 4 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bayascas, E Castillo, A. Munoz-Marmol, and E Salo Planarian Hox genes: novel patterns of expression during regeneration Development, January 1, 1997; 124(1): 141 - 148. [Abstract] [PDF]
|
|
|
|
|
|
J. A. White, Y.-D. Guo, K. Baetz, B. Beckett-Jones, J. Bonasoro, K. E. Hsu, F. J. Dilworth, G. Jones, and M. Petkovich Identification of the Retinoic Acid-inducible All-trans-retinoic Acid 4-Hydroxylase J. Biol. Chem., November 22, 1996; 271(47): 29922 - 29927. [Abstract] [Full Text] [PDF]
|
|
