|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 2 371-381, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
DL Paul, K Yu, R Bruzzone, RL Gimlich and DA Goodenough
Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
A chimeric construct, termed 3243H7, composed of fused portions of the rat gap junction proteins connexin32 (Cx32) and connexin43 (Cx43) has been shown to have selective dominant inhibitory activity when tested in the Xenopus oocyte pair system. Co-injection of mRNA coding for 3243H7 together with mRNAs coding for Cx32 or Cx43 completely blocked the development of channel conductances, while the construct was ineffective at blocking intercellular channel assembly when coinjected with rat connexin37 (Cx37). Injection of 3243H7 into the right anterodorsal blastomere of 8-cell-stage Xenopus embryos resulted in disadhesion and delamination of the resultant clone of cells evident by embryonic stage 8; a substantial number, although not all, of the progeny of the injected cell were eliminated from the embryo by stage 12. A second construct, 3243H8, differing from 3243H7 in the relative position of the middle splice, had no dominant negative activity in the oocyte pair assay, nor any detectable effects on Xenopus development, even when injected at four-fold higher concentrations. The 3243H7-induced embryonic defects could be rescued by coinjection of Cx37 with 3243H7. A blastomere reaggregation assay was used to demonstrate that a depression of dye-transfer could be detected in 3243H7-injected cells as early as stage 7; Lucifer yellow injections into single cells also demonstrated that injection of 3243H7 resulted in a block of intercellular communication. These experiments indicate that maintenance of embryonic cell adhesion with concomitant positional information requires gap junction-mediated intercellular communication.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Dale Dynamic ATP signalling and neural development J. Physiol., May 15, 2008; 586(10): 2429 - 2436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Calera, H. L. Topley, Y. Liao, B. R. Duling, D. L. Paul, and D. A. Goodenough Connexin43 is required for production of the aqueous humor in the murine eye J. Cell Sci., November 1, 2006; 119(21): 4510 - 4519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-J. Wei, R. Francis, X. Xu, and C. W. Lo Connexin43 Associated with an N-cadherin-containing Multiprotein Complex Is Required for Gap Junction Formation in NIH3T3 Cells J. Biol. Chem., May 20, 2005; 280(20): 19925 - 19936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kroschewski Molecular Mechanisms of Epithelial Polarity: About Shapes, Forces, and Orientation Problems Physiology, April 1, 2004; 19(2): 61 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. El-Sabban, A. J. Sfeir, M. H. Daher, N. Y. Kalaany, R. A. Bassam, and R. S. Talhouk ECM-induced gap junctional communication enhances mammary epithelial cell differentiation J. Cell Sci., September 1, 2003; 116(17): 3531 - 3541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Das Sarma, R. A. Meyer, F. Wang, V. Abraham, C. W. Lo, and M. Koval Multimeric connexin interactions prior to the trans-Golgi network J. Cell Sci., March 13, 2002; 114(22): 4013 - 4024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Kwak, M. S. Pepper, D. B. Gros, and P. Meda Inhibition of Endothelial Wound Repair by Dominant Negative Connexin Inhibitors Mol. Biol. Cell, April 1, 2001; 12(4): 831 - 845. [Abstract] [Full Text]
|
|
|
|
|
|
Y Landesman, T. White, T. Starich, J. Shaw, D. Goodenough, and D. Paul Innexin-3 forms connexin-like intercellular channels J. Cell Sci., January 7, 1999; 112(14): 2391 - 2396. [Abstract] [PDF]
|
|
|
|
 |
|
 C. I. Foote, L. Zhou, X. Zhu, and B. J. Nicholson The Pattern of Disulfide Linkages in the Extracellular Loop Regions of Connexin 32 Suggests a Model for the Docking Interface of Gap Junctions J. Cell Biol., March 9, 1998; 140(5): 1187 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. M. Martin, C. H. George, C. Castro, J. M. Kendall, J. Capel, A. K. Campbell, A. Revilla, L. C. Barrio, and W. H. Evans Assembly of Chimeric Connexin-Aequorin Proteins into Functional Gap Junction Channels. REPORTING INTRACELLULAR AND PLASMA MEMBRANE CALCIUM ENVIRONMENTS J. Biol. Chem., January 16, 1998; 273(3): 1719 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-h. Chen, C. Merzdorf, D. L. Paul, and D. A. Goodenough COOH Terminus of Occludin Is Required for Tight Junction Barrier Function in Early Xenopus Embryos J. Cell Biol., August 25, 1997; 138(4): 891 - 899. [Abstract] [Full Text] [PDF]
|
|
