|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 2 569-578, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
MJ Sonnenfeld and JR Jacobs
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
We have examined cell death within lineages in the midline of Drosophila embryos. Approximately 50% of cells within the anterior, middle and posterior midline glial (MGA, MGM and MGP) lineages died by apoptosis after separation of the commissural axon tracts. Glial apoptosis is blocked in embryos deficient for reaper, where greater than wild-type numbers of midline glia (MG) are present after stage 12. Quantitative studies revealed that MG death followed a consistent temporal pattern during embryogenesis. Apoptotic MG were expelled from the central nervous system and were subsequently engulfed by phagocytic haemocytes. MGA and MGM survival was apparently dependent upon proper axonal contact. In embryos mutant for the commissureless gene, a decrease in axon-glia contact correlated with a decrease in MGA and MGM survival and accelerated the time course of MG death. In embryos mutant for the slit gene, MGA and MGM maintained contact with longitudinally and contralaterally projecting axons and MG survival was comparable to that in wild-type embryos. The initial number of MG within individual ventral nerve cord segments was increased by ectopic expression of the rhomboid gene, without changing axon number. Extra MGA and MGM were eliminated from the ventral nerve cord by apoptosis to restore wild-type numbers of midline glia. Ectopic rhomboid expression also shifted MGA and MGM cell death to an earlier stage of embryogenesis. One possible explanation is that axon-glia contact or communication promotes survival of the MG and that MG death may result from a competition for available axon contact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Stork, S. Thomas, F. Rodrigues, M. Silies, E. Naffin, S. Wenderdel, and C. Klambt Drosophila Neurexin IV stabilizes neuron-glia interactions at the CNS midline by binding to Wrapper Development, April 15, 2009; 136(8): 1251 - 1261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Wheeler, S. Banerjee, K. Blauth, S. L. Rogers, M. A. Bhat, and S. T. Crews Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment Development, April 1, 2009; 136(7): 1147 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Muro, D. L. Berry, J. R. Huh, C. H. Chen, H. Huang, S. J. Yoo, M. Guo, E. H. Baehrecke, and B. A. Hay The Drosophila caspase Ice is important for many apoptotic cell deaths and for spermatid individualization, a nonapoptotic process Development, September 1, 2006; 133(17): 3305 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xu, Y. Li, M. Arcaro, M. Lackey, and A. Bergmann The CARD-carrying caspase Dronc is essential for most, but not all, developmental cell death in Drosophila Development, May 1, 2005; 132(9): 2125 - 2134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Sepp and V. J. Auld Reciprocal Interactions between Neurons and Glia Are Required for Drosophila Peripheral Nervous System Development J. Neurosci., September 10, 2003; 23(23): 8221 - 8230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-J. Lee, D. A. Stephenson, M. J. Groves, M. G. Sweeney, M. B. Davis, S.-F. An, H. Houlden, M. A. M. Salih, V. Timmerman, P. de Jonghe, et al. Hereditary sensory neuropathy is caused by a mutation in the delta subunit of the cytosolic chaperonin-containing t-complex peptide-1 (Cct4 ) gene Hum. Mol. Genet., August 1, 2003; 12(15): 1917 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Sears, C. J. Kennedy, and P. A. Garrity Macrophage-mediated corpse engulfment is required for normal Drosophila CNS morphogenesis Development, August 1, 2003; 130(15): 3557 - 3565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Besse and A.-M. Pret Apoptosis-mediated cell death within the ovarian polar cell lineage of Drosophila melanogaster Development, March 1, 2003; 130(5): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gorski and M. Marra Programmed cell death takes flight: genetic and genomic approaches to gene discovery in Drosophila Physiol Genomics, May 10, 2002; 9(2): 59 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sedaghat, W. F. Miranda, and M. J. Sonnenfeld The jing Zn-finger transcription factor is a mediator of cellular differentiation in the Drosophila CNS midline and trachea Development, January 6, 2002; 129(11): 2591 - 2606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Kramer, M Okabe, N Hacohen, M. Krasnow, and Y Hiromi Sprouty: a common antagonist of FGF and EGF signaling pathways in Drosophila Development, January 6, 1999; 126(11): 2515 - 2525. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Zhou, A. Schnitzler, J. Agapite, L. M. Schwartz, H. Steller, and J. R. Nambu Cooperative functions of the reaper and head involution defective genes in the programmed cell death of Drosophila central nervous system midline cells PNAS, May 13, 1997; 94(10): 5131 - 5136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Stemerdink and J. Jacobs Argos and Spitz group genes function to regulate midline glial cell number in Drosophila embryos Development, January 10, 1997; 124(19): 3787 - 3796. [Abstract] [PDF]
|
|
|
|
|
|
D. Ciutat, J. Caldero, R. W. Oppenheim, and J. E. Esquerda Schwann Cell Apoptosis during Normal Development and after Axonal Degeneration Induced by Neurotoxins in the Chick Embryo J. Neurosci., June 15, 1996; 16(12): 3979 - 3990. [Abstract] [Full Text] [PDF]
|
|
