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Development, Vol 121, Issue 4 1111-1122, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
DG Wang, CV Kirchhamer, RJ Britten and EH Davidson
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
The CyIIIa cytoskeletal actin gene of the sea urchin Strongylocentrotus purpuratus is activated in late cleavage and expressed exclusively in the aboral ectoderm territory of the embryo. Previous gene transfer studies defined a 2.3 kb cis-regulatory region that is necessary and sufficient for correct temporal and spatial expression of a CyIIIa.CAT fusion gene. In this paper, a negative regulatory element within this region was identified that is required for repression of the CyIIIa gene in skeletogenic mesenchyme cells. The repression mediated by this regulatory element takes place after initial territorial specification. A cDNA clone encoding a DNA-binding protein with twelve Zn fingers (SpZ12-1) was isolated by probing an expression library with this cis-element. Deletion analysis of the SpZ12-1 protein confirmed that a DNA-binding domain is located within the Zn finger region. SpZ12-1 is the only DNA-binding protein in embryo nuclear extract that interacts with the specific cis-target sites required for repression of CyIIIa.CAT in skeletogenic mesenchyme and is likely to be the trans factor that mediates this repression.
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M. Arnone and E. Davidson The hardwiring of development: organization and function of genomic regulatory systems Development, January 5, 1997; 124(10): 1851 - 1864. [Abstract] [PDF]
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C. Mao, A. Wikramanayake, L Gan, C. Chuang, R. Summers, and W. Klein Altering cell fates in sea urchin embryos by overexpressing SpOtx, an orthodenticle-related protein Development, January 5, 1996; 122(5): 1489 - 1498. [Abstract] [PDF]
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C. Kirchhamer and E. Davidson Spatial and temporal information processing in the sea urchin embryo: modular and intramodular organization of the CyIIIa gene cis-regulatory system Development, January 1, 1996; 122(1): 333 - 348. [Abstract] [PDF]
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K. Makabe, C. Kirchhamer, R. Britten, and E. Davidson Cis-regulatory control of the SM50 gene, an early marker of skeletogenic lineage specification in the sea urchin embryo Development, January 7, 1995; 121(7): 1957 - 1970. [Abstract] [PDF]
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