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Development, Vol 121, Issue 4 1129-1137, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation

PE Visconti, JL Bailey, GD Moore, D Pan, P Olds-Clarke and GS Kopf
Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104-6080, USA.

The molecular basis of mammalian sperm capacitation, defined functionally as those processes that confer on the sperm the acquisition of fertilization-competence either in vivo in the female reproductive tract or in vitro, is poorly understood. We demonstrate here that capacitation of caudal epididymal mouse sperm in vitro is accompanied by a time-dependent increase in the protein tyrosine phosphorylation of a subset of proteins of M(r) 40,000-120,000. Incubation of sperm in media devoid of bovine serum albumin, CaCl2 or NaHCO3, components which individually are required for capacitation, prevent the sperm from undergoing capacitation as assessed by the ability of the cells to acquire the pattern B chlortetracycline fluorescence, to undergo the zona pellucida-induced acrosome reaction and, in some cases, to fertilize metaphase II-arrested eggs in vitro. In each of these cases the protein tyrosine phosphorylation of the subset of capacitation-associated proteins does not occur. Protein tyrosine phosphorylation of these particular proteins, as well as sperm capacitation, can be recovered in media devoid of each of these three constituents (bovine serum albumin, CaCl2 or NaHCO3) by adding back the appropriate component in a concentration-dependent manner. The requirement of NaHCO3 for these phosphorylations is not due to an alkalinization of intracellular sperm pH or to an increase in media pH. Caput epididymal sperm, which lack the ability to undergo capacitation in vitro, do not display this capacitation-dependent subset of tyrosine phosphorylated proteins in complete media even after extended incubation periods, and do not fertilize metaphase II-arrested eggs in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
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I. Ben-Aharon, P. R Brown, N. Etkovitz, E. M Eddy, and R. Shalgi
The expression of calpain 1 and calpain 2 in spermatogenic cells and spermatozoa of the mouse
Reproduction, April 1, 2005; 129(4): 435 - 442.
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 Biol. Reprod.Home page
A. Furimsky, N. Vuong, H. Xu, P. Kumarathasan, M. Xu, W. Weerachatyanukul, M. Bou Khalil, M. Kates, and N. Tanphaichitr
Percoll Gradient-Centrifuged Capacitated Mouse Sperm Have Increased Fertilizing Ability and Higher Contents of Sulfogalactosylglycerolipid and Docosahexaenoic Acid-Containing Phosphatidylcholine Compared to Washed Capacitated Mouse Sperm
Biol Reprod, March 1, 2005; 72(3): 574 - 583.
[Abstract] [Full Text] [PDF]

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 ReproductionHome page
R. Gualtieri, R. Boni, E. Tosti, M. Zagami, and R. Talevi
Intracellular calcium and protein tyrosine phosphorylation during the release of bovine sperm adhering to the fallopian tube epithelium in vitro
Reproduction, January 1, 2005; 129(1): 51 - 60.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
M. Luconi, I. Porazzi, P. Ferruzzi, S. Marchiani, G. Forti, and E. Baldi
Tyrosine Phosphorylation of the A Kinase Anchoring Protein 3 (AKAP3) and Soluble Adenylate Cyclase Are Involved in the Increase of Human Sperm Motility by Bicarbonate
Biol Reprod, January 1, 2005; 72(1): 22 - 32.
[Abstract] [Full Text] [PDF]

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 J AndrolHome page
S. S. Baker, M. Thomas, and C. D. Thaler
Sperm Membrane Dynamics Assessed by Changes in Lectin Fluorescence Before and After Capacitation
J Androl, September 1, 2004; 25(5): 744 - 751.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
W. F. Hanna, C. L. Kerr, J. H. Shaper, and W. W. Wright
Lewis X-Containing Neoglycoproteins Mimic the Intrinsic Ability of Zona Pellucida Glycoprotein ZP3 to Induce the Acrosome Reaction in Capacitated Mouse Sperm
Biol Reprod, September 1, 2004; 71(3): 778 - 789.
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 Biol. Reprod.Home page
J. Seligman, Y. Zipser, and N. S. Kosower
Tyrosine Phosphorylation, Thiol Status, and Protein Tyrosine Phosphatase in Rat Epididymal Spermatozoa
Biol Reprod, September 1, 2004; 71(3): 1009 - 1015.
[Abstract] [Full Text] [PDF]

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 J. Cell Sci.Home page
K. L. Asquith, R. M. Baleato, E. A. McLaughlin, B. Nixon, and R. J. Aitken
Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition
J. Cell Sci., July 15, 2004; 117(16): 3645 - 3657.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
A. J. Travis, L. Tutuncu, C. J. Jorgez, T. S. Ord, B. H. Jones, G. S. Kopf, and C. J. Williams
Requirements for Glucose Beyond Sperm Capacitation During In Vitro Fertilization in the Mouse
Biol Reprod, July 1, 2004; 71(1): 139 - 145.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
S. Shadan, P. S. James, E. A. Howes, and R. Jones
Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa
Biol Reprod, July 1, 2004; 71(1): 253 - 265.
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 Mol Hum ReprodHome page
Z. Hao, K. N. Jha, Y.-H. Kim, S. Vemuganti, V.A. Westbrook, O. Chertihin, K. Markgraf, C. J. Flickinger, M. Coppola, J. C. Herr, et al.
Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm
Mol. Hum. Reprod., June 1, 2004; 10(6): 433 - 444.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
V. G. Da Ros, M. J. Munuce, D. J. Cohen, C. I. Marin-Briggiler, D. Busso, P. E. Visconti, and P. S. Cuasnicu
Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
Biol Reprod, May 1, 2004; 70(5): 1325 - 1332.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
K. Mitra and S. Shivaji
Novel Tyrosine-Phosphorylated Post-Pyruvate Metabolic Enzyme, Dihydrolipoamide Dehydrogenase, Involved in Capacitation of Hamster Spermatozoa
Biol Reprod, April 1, 2004; 70(4): 887 - 899.
[Abstract] [Full Text] [PDF]

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 J AndrolHome page
A. Minelli, L. Liguori, I. Bellazza, R. Mannucci, B. Johansson, and B. B. Fredholm
Involvement of A1 Adenosine Receptors in the Acquisition of Fertilizing Capacity
J Androl, March 1, 2004; 25(2): 286 - 292.
[Abstract] [Full Text] [PDF]

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 DevelopmentHome page
C. Rodeheffer and B. D. Shur
Sperm from {beta}1,4-galactosyltransferase I-null mice exhibit precocious capacitation
Development, February 1, 2004; 131(3): 491 - 501.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
J. Rivlin, J. Mendel, S. Rubinstein, N. Etkovitz, and H. Breitbart
Role of Hydrogen Peroxide in Sperm Capacitation and Acrosome Reaction
Biol Reprod, February 1, 2004; 70(2): 518 - 522.
[Abstract] [Full Text] [PDF]

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 J. Cell Sci.Home page
M. A. Baker, L. Hetherington, H. Ecroyd, S. D. Roman, and R. J. Aitken
Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation
J. Cell Sci., January 15, 2004; 117(2): 211 - 222.
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 Hum ReprodHome page
M.G. Buffone, G.F. Doncel, C.I. Marin Briggiler, M.H. Vazquez-Levin, and J.C. Calamera
Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation
Hum. Reprod., January 1, 2004; 19(1): 139 - 146.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
H. Ecroyd, R. C. Jones, and R. J. Aitken
Tyrosine Phosphorylation of HSP-90 During Mammalian Sperm Capacitation
Biol Reprod, December 1, 2003; 69(6): 1801 - 1807.
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 Mol Hum ReprodHome page
R.J. Aitken, A.L. Ryan, B.J. Curry, and M.A. Baker
Multiple forms of redox activity in populations of human spermatozoa
Mol. Hum. Reprod., November 1, 2003; 9(11): 645 - 661.
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 J BiochemHome page
M. Fujinoki, T. Kawamura, T. Toda, H. Ohtake, N. Shimizu, S. Yamaoka, and M. Okuno
Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa
J. Biochem., October 1, 2003; 134(4): 559 - 565.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
B. S. Jaiswal and M. Conti
Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa
PNAS, September 16, 2003; 100(19): 10676 - 10681.
[Abstract] [Full Text] [PDF]

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 J AndrolHome page
C. Dube, S. Tardif, P. Leclerc, and J. L. Bailey
The Importance of Calcium in the Appearance of p32, a Boar Sperm Tyrosine Phosphoprotein, During In Vitro Capacitation
J Androl, September 1, 2003; 24(5): 727 - 733.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
J. Shetty, M. J. Wolkowicz, L. C. Digilio, K. L. Klotz, F. L. Jayes, A. B. Diekman, V. A. Westbrook, E. M. Farris, Z. Hao, S. A. Coonrod, et al.
SAMP14, a Novel, Acrosomal Membrane-associated, Glycosylphosphatidylinositol-anchored Member of the Ly-6/Urokinase-type Plasminogen Activator Receptor Superfamily with a Role in Sperm-Egg Interaction
J. Biol. Chem., August 15, 2003; 278(33): 30506 - 30515.
[Abstract] [Full Text] [PDF]

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 Hum ReprodHome page
S. Aquila, D. Sisci, M. Gentile, A. Carpino, E. Middea, S. Catalano, V. Rago, and S. Ando
Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm
Hum. Reprod., August 1, 2003; 18(8): 1650 - 1659.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
K. P. Roberts, J. A. Wamstad, K. M. Ensrud, and D. W. Hamilton
Inhibition of Capacitation-Associated Tyrosine Phosphorylation Signaling in Rat Sperm by Epididymal Protein Crisp-1
Biol Reprod, August 1, 2003; 69(2): 572 - 581.
[Abstract] [Full Text] [PDF]

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 J AndrolHome page
E. O. Pietrobon, M. De Los Angeles Monclus, A. J. Alberdi, and M. W. Fornes
Progesterone Receptor Availability in Mouse Spermatozoa During Epididymal Transit and Capacitation: Ligand Blot Detection of Progesterone-Binding Protein
J Androl, July 1, 2003; 24(4): 612 - 620.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
N. Cormier and J. L. Bailey
A Differential Mechanism Is Involved During Heparin- and Cryopreservation-Induced Capacitation of Bovine Spermatozoa
Biol Reprod, July 1, 2003; 69(1): 177 - 185.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
H. W. Ecroyd, R. C. Jones, and R. J. Aitken
Endogenous Redox Activity in Mouse Spermatozoa and Its Role in Regulating the Tyrosine Phosphorylation Events Associated with Sperm Capacitation
Biol Reprod, July 1, 2003; 69(1): 347 - 354.
[Abstract] [Full Text] [PDF]

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 JGPHome page
G. Wennemuth, D. F. Babcock, and B. Hille
Calcium Clearance Mechanisms of Mouse Sperm
J. Gen. Physiol., June 30, 2003; 122(1): 115 - 128.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
K.J. de Vries, T. Wiedmer, P.J. Sims, and B.M. Gadella
Caspase-Independent Exposure of Aminophospholipids and Tyrosine Phosphorylation in Bicarbonate Responsive Human Sperm Cells
Biol Reprod, June 1, 2003; 68(6): 2122 - 2134.
[Abstract] [Full Text] [PDF]

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H.-T. Zeng and D. R. P. Tulsiani
Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components
J. Cell Sci., May 15, 2003; 116(10): 1981 - 1989.
[Abstract] [Full Text] [PDF]

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 J AndrolHome page
A. M. Petrunkina, K. Simon, A.-R. Gunzel-Apel, and E. Topfer-Petersen
Specific Order in the Appearance of Protein Tyrosine Phosphorylation Patterns Is Functionally Coordinated With Dog Sperm Hyperactivation and Capacitation
J Androl, May 1, 2003; 24(3): 423 - 437.
[Abstract] [Full Text] [PDF]

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 JCBHome page
K. Fukami, M. Yoshida, T. Inoue, M. Kurokawa, R. A. Fissore, N. Yoshida, K. Mikoshiba, and T. Takenawa
Phospholipase C{delta}4 is required for Ca2+ mobilization essential for acrosome reaction in sperm
J. Cell Biol., April 14, 2003; 161(1): 79 - 88.
[Abstract] [Full Text] [PDF]

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 DevelopmentHome page
G. Wennemuth, A. E. Carlson, A. J. Harper, and D. F. Babcock
Bicarbonate actions on flagellar and Ca2+-channel responses: initial events in sperm activation
Development, April 1, 2003; 130(7): 1317 - 1326.
[Abstract] [Full Text] [PDF]

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 Biol. Reprod.Home page
D. Sakkas, G. Leppens-Luisier, H. Lucas, D. Chardonnens, A. Campana, D.R. Franken, and F. Urner
Localization of Tyrosine Phosphorylated Proteins in Human Sperm and Relation to Capacitation and Zona Pellucida Binding
Biol Reprod, April 1, 2003; 68(4): 1463 - 1469.
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A. C. Pommer, J. Rutllant, and S. A. Meyers
Phosphorylation of Protein Tyrosine Residues in Fresh and Cryopreserved Stallion Spermatozoa under Capacitating Conditions
Biol Reprod, April 1, 2003; 68(4): 1208 - 1214.
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S. Ficarro, O. Chertihin, V. A. Westbrook, F. White, F. Jayes, P. Kalab, J. A. Marto, J. Shabanowitz, J. C. Herr, D. F. Hunt, et al.
Phosphoproteome Analysis of Capacitated Human Sperm. EVIDENCE OF TYROSINE PHOSPHORYLATION OF A KINASE-ANCHORING PROTEIN 3 AND VALOSIN-CONTAINING PROTEIN/p97 DURING CAPACITATION
J. Biol. Chem., March 21, 2003; 278(13): 11579 - 11589.
[Abstract] [Full Text] [PDF]


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