spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fontaine-Perus, J.
Right arrow Articles by Paulin, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fontaine-Perus, J.
Right arrow Articles by Paulin, D.

Development, Vol 121, Issue 6 1705-1718, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Mouse chick chimera: a new model to study the in ovo developmental potentialities of mammalian somites

J Fontaine-Perus, V Jarno, C Fournier le Ray, Z Li and D Paulin
CNRS URA 1340, Faculte des Sciences et des Techniques, Nantes, France.

Chimeras were prepared by transplanting somites from 9-day post-coitum mouse embryos or somitic dermomyotomes from 10-day post-coitum mouse embryos into 2-day-old chick embryos at different axial levels. Mouse somitic cells then differentiated in ovo in dermis, cartilage and skeletal muscle as they normally do in the course of development and were able to migrate into chick host limb. To trace the behavior of somitic myogenic stem cells more closely, somites arising from mice bearing a transgene of the desmin gene linked to a reporter gene coding for Escherichia coli beta-galactosidase (lacZ) were grafted in ovo. Interestingly, the transgene was rapidly expressed in myotomal muscles derived from implants. In the limb muscle mass, positive cells were found several days after implantation. Activation of desmin nls lacZ also occurred in in vitro cultures of somite-derived cells. Our experimental method facilitates investigation of the mechanisms of mammalian development, allowing the normal fate of implanted mouse cells to be studied and providing suitable conditions for identification of descendants of genetically modified cells.


This article has been cited by other articles:


Home page
 DevelopmentHome page
D. Houzelstein, Y. Cheraud, G. Auda-Boucher, J. Fontaine-Perus, and B. Robert
The expression of the homeobox gene Msx1 reveals two populations of dermal progenitor cells originating from the somites
Development, May 15, 2000; 127(10): 2155 - 2164.
[Abstract] [PDF]

Home page
 DevelopmentHome page
D Houzelstein, G Auda-Boucher, Y Cheraud, T Rouaud, I Blanc, S Tajbakhsh, M. Buckingham, J Fontaine-Perus, and B Robert
The homeobox gene Msx1 is expressed in a subset of somites, and in muscle progenitor cells migrating into the forelimb
Development, January 6, 1999; 126(12): 2689 - 2701.
[Abstract] [PDF]

Home page
 DevelopmentHome page
P. Gruber, S. Kubalak, and K. Chien
Downregulation of atrial markers during cardiac chamber morphogenesis is irreversible in murine embryos
Development, January 11, 1998; 125(22): 4427 - 4438.
[Abstract] [PDF]

Home page
 DevelopmentHome page
J Fontaine-Perus, P Halgand, Y Cheraud, T Rouaud, M. Velasco, C Cifuentes Diaz, and F Rieger
Mouse-chick chimera: a developmental model of murine neurogenic cells
Development, January 8, 1997; 124(16): 3025 - 3036.
[Abstract] [PDF]

Home page
 DevelopmentHome page
W. Denetclaw, B Christ, and C. Ordahl
Location and growth of epaxial myotome precursor cells
Development, January 4, 1997; 124(8): 1601 - 1610.
[Abstract] [PDF]


© The Company of Biologists Ltd 1995