|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 8 2559-2567, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
P Souza, M Kuliszewski, J Wang, I Tseu, AK Tanswell and M Post
Neonatal Research Division, Hospital for Sick Children Research Institute, University of Toronto, Ontario, Canada.
The biological role of platelet-derived growth factor (PDGF)-AA in lung morphogenesis was investigated by incubating embryonic lung explants with phosphorothioate antisense PDGF-A oligonucleotides, which decreased PDGF-AA but not PDGF-BB protein content. Antisense PDGF-A oligonucleotides inhibited DNA synthesis. This inhibitory effect of antisense PDGF-A was reversed by the addition of exogenous PDGF-AA but not PDGF-BB. Morphometric analyses of antisense-treated cultures showed a significant reduction in lung size. The number of terminal buds of the lung explants was significantly decreased by antisense PDGF-A oligonucleotides. PDGF-AA but not PDGF-BB attenuated the inhibitory effect of antisense PDGF-A on early lung branching. Sense PDGF-A had no effect on DNA synthesis and early lung branching. Reverse transcriptase-polymerase chain reaction analysis revealed PDGF-A mRNA expression in the epithelial component of the embryonic lung, while message for PDGF alpha-receptor was expressed in the mesenchyme. Incubation of explants with neutralizing PDGF-AA antibodies also reduced DNA synthesis and early branching morphogenesis. We conclude that PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.
This article has been cited by other articles:
|
|
 |
|
  D. E. Kling, H. K. Lorenzo, A. M. Trbovich, T. B. Kinane, P. K. Donahoe, and J. J. Schnitzer Pre- and Postnatal Lung Development, Maturation, and Plasticity: MEK-1/2 inhibition reduces branching morphogenesis and causes mesenchymal cell apoptosis in fetal rat lungs Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L370 - L378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Lindroos, Y.-Z. Wang, A. B. Rice, and J. C. Bonner Regulation of PDGFR-{alpha} in rat pulmonary myofibroblasts by staurosporine Am J Physiol Lung Cell Mol Physiol, February 1, 2001; 280(2): L354 - L362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Oster, W. W. Marhin, C. Asker, L. M. Facchini, P. A. Dion, K. Funa, M. Post, J. M. Sedivy, and L. Z. Penn Myc Is an Essential Negative Regulator of Platelet-Derived Growth Factor Beta Receptor Expression Mol. Cell. Biol., September 15, 2000; 20(18): 6768 - 6778. [Abstract] [Full Text]
|
|
|
|
 |
|
 T Sun, D Jayatilake, G. Afink, P Ataliotis, M Nister, W. Richardson, and H. Smith A human YAC transgene rescues craniofacial and neural tube development in PDGFRalpha knockout mice and uncovers a role for PDGFRalpha in prenatal lung growth Development, January 11, 2000; 127(21): 4519 - 4529. [Abstract] [PDF]
|
|
|
|
 |
|
 C.-H. Heldin and B. Westermark Mechanism of Action and In Vivo Role of Platelet-Derived Growth Factor Physiol Rev, October 1, 1999; 79(4): 1283 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Lindroos, A. B. Rice, Y.-Z. Wang, and J. C. Bonner Role of Nuclear Factor-{kappa}B and Mitogen-Activated Protein Kinase Signaling Pathways in IL-1{beta}-Mediated Induction of {alpha}-PDGF Receptor Expression in Rat Pulmonary Myofibroblasts J. Immunol., October 1, 1998; 161(7): 3464 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Ohmichi, U Koshimizu, K Matsumoto, and T Nakamura Hepatocyte growth factor (HGF) acts as a mesenchyme-derived morphogenic factor during fetal lung development Development, January 4, 1998; 125(7): 1315 - 1324. [Abstract] [PDF]
|
|
|
|
|
|
M Post, P Souza, J Liu, I Tseu, J Wang, M Kuliszewski, and A. Tanswell Keratinocyte growth factor and its receptor are involved in regulating early lung branching Development, January 10, 1996; 122(10): 3107 - 3115. [Abstract] [PDF]
|
|
