|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 1 391-404, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
H Browning and S Strome
Department of Biology, Indiana University, Bloomington 47405, USA.
The paternal-effect embryonic-lethal gene, spe-11, is required for normal development of early C. elegans embryos. Spe-11 embryos fail to complete meiosis, form a weak eggshell, fail to orient properly the first mitotic spindle, and fail to undergo cytokinesis. Here we report cloning and sequencing of the spe-11 gene, which encodes a novel protein. As predicted by the paternal-effect mutant phenotype, the gene is expressed during spermatogenesis but is not detectable in females undergoing oogenesis, and the protein is present in mature sperm. To investigate whether SPE-11's essential function is during spermatogenesis or whether sperm-delivered SPE-11 functions in the newly fertilized embryo, we engineered animals to supply SPE-11 to the embryo through the oocyte rather than through the sperm. We found that maternal expression is sufficient for embryonic viability. This result demonstrates that SPE-11 is not required during spermatogenesis, and suggests that SPE-11 is a sperm-supplied factor that participates directly in development of the early embryo. In contrast to the many known maternal factors required for embryogenesis, SPE-11 is the first paternally contributed factor to be genetically identified and molecularly characterized.
This article has been cited by other articles:
|
|
 |
|
  H. S. Seidel, M. V. Rockman, and L. Kruglyak Widespread Genetic Incompatibility in C. Elegans Maintained by Balancing Selection Science, February 1, 2008; 319(5863): 589 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Darby, A. Chakraborti, S. M. Politz, C. C. Daniels, L. Tan, and K. Drace Caenorhabditis elegans Mutants Resistant to Attachment of Yersinia Biofilms Genetics, May 1, 2007; 176(1): 221 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lyczak, L. Zweier, T. Group, M. A. Murrow, C. Snyder, L. Kulovitz, A. Beatty, K. Smith, and B. Bowerman The puromycin-sensitive aminopeptidase PAM-1 is required for meiotic exit and anteroposterior polarity in the one-cell Caenorhabditis elegans embryo Development, November 1, 2006; 133(21): 4281 - 4292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Gleason, W. C. Lindsey, T. L. Kroft, A. W. Singson, and S. W. L'Hernault spe-10 Encodes a DHHC-CRD Zinc-Finger Membrane Protein Required for Endoplasmic Reticulum/Golgi Membrane Morphogenesis During Caenorhabditis elegans Spermatogenesis Genetics, January 1, 2006; 172(1): 145 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Han, Y. G. Chung, S. Gao, and K. E. Latham Maternal Factors Controlling Blastomere Fragmentation in Early Mouse Embryos Biol Reprod, March 1, 2005; 72(3): 612 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sasao, N. Itoh, H. Takano, S. Watanabe, G. Wei, T. Tsukamoto, N. Kuzumaki, and M. Takimoto The protein encoded by cancer/testis gene D40/AF15q14 is localized in spermatocytes, acrosomes of spermatids and ejaculated spermatozoa Reproduction, December 1, 2004; 128(6): 709 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Shi, A. Z. Wu, M. Santos, Z.-M. Feng, L. Huang, Y.-M. Chen, K. Zhu, and C.-L. C. Chen Cloning and Characterization of Rat Spermatid Protein SSP411: A Thioredoxin-Like Protein J Androl, July 1, 2004; 25(4): 479 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matsubara, Khin-Thidar, and Y. Sano A Gene Block Causing Cross-Incompatibility Hidden in Wild and Cultivated Rice Genetics, September 1, 2003; 165(1): 343 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Padilla, T. G. Nystul, R. A. Zager, A. C.M. Johnson, and M. B. Roth Dephosphorylation of Cell Cycle-regulated Proteins Correlates with Anoxia-induced Suspended Animation in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2002; 13(5): 1473 - 1483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gruenbaum, K. K. Lee, J. Liu, M. Cohen, and K. L. Wilson The expression, lamin-dependent localization and RNAi depletion phenotype for emerin in C. elegans J. Cell Sci., January 3, 2002; 115(5): 923 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Hawes, Y. Gie Chung, and K. E. Latham Genetic and Epigenetic Factors Affecting Blastomere Fragmentation in Two-Cell Stage Mouse Embryos Biol Reprod, October 1, 2001; 65(4): 1050 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Browning, J. Hayles, J. Mata, L. Aveline, P. Nurse, and J. R. McIntosh Tea2p Is a Kinesin-like Protein Required to Generate Polarized Growth in Fission Yeast J. Cell Biol., September 25, 2000; 151(1): 15 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Lee, Y. Gruenbaum, P. Spann, J. Liu, and K. L. Wilson C. elegans Nuclear Envelope Proteins Emerin, MAN1, Lamin, and Nucleoporins Reveal Unique Timing of Nuclear Envelope Breakdown during Mitosis Mol. Biol. Cell, September 1, 2000; 11(9): 3089 - 3099. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Presgraves A Genetic Test of the Mechanism of Wolbachia-Induced Cytoplasmic Incompatibility in Drosophila Genetics, February 1, 2000; 154(2): 771 - 776. [Abstract] [Full Text]
|
|
|
|
|
|
P. Sadler and D. Shakes Anucleate Caenorhabditis elegans sperm can crawl, fertilize oocytes and direct anterior-posterior polarization of the 1-cell embryo Development, January 1, 2000; 127(2): 355 - 366. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Jin, E. Jorgensen, E. Hartwieg, and H. R. Horvitz The Caenorhabditis elegans Gene unc-25 Encodes Glutamic Acid Decarboxylase and Is Required for Synaptic Transmission But Not Synaptic Development J. Neurosci., January 15, 1999; 19(2): 539 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M&; G. Mahoney, W. Tang, M. M. Xiang, S. B. Moss, G. L. Gerton, J. R. Stanley, and H. Tseng Translocation of the Zinc Finger Protein Basonuclin from the Mouse Germ Cell Nucleus to the Midpiece of the Spermatozoon during Spermiogenesis Biol Reprod, August 1, 1998; 59(2): 388 - 394. [Abstract] [Full Text]
|
|
|
|
|
|
G Herrada and D. Wolgemuth The mouse transcription factor Stat4 is expressed in haploid male germ cells and is present in the perinuclear theca of spermatozoa J. Cell Sci., January 7, 1997; 110(14): 1543 - 1553. [Abstract] [PDF]
|
|
|
|
 |
|
 I D Chin-Sang and A M Spence Caenorhabditis elegans sex-determining protein FEM-2 is a protein phosphatase that promotes male development and interacts directly with FEM-3. Genes & Dev., September 15, 1996; 10(18): 2314 - 2325. [Abstract] [PDF]
|
|
