Development, Vol 122, Issue 2 527-536, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

eagle, a member of the steroid receptor gene superfamily, is expressed in a subset of neuroblasts and regulates the fate of their putative progeny in the Drosophila CNS

S Higashijima, E Shishido, M Matsuzaki and K Saigo
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.

We isolated and characterized the eagle gene, encoding a member of the steroid receptor superfamily in Drosophila. In the central nervous system eagle RNA was expressed in a limited number of cells. During stages 10 and 11, eagle RNA expression was observed in four neuroblasts, NB2-4, NB3-3, NB6-4 and NB7-3. Except for NB6-4, eagle RNA expression reached a maximum at the very beginning of expression or in the period of neuroblast delamination. Weak eagle RNA expression was also observed in a few putative progeny of NB7-3 during stages, late 11 and 12. All eagle RNA in abdominal segments disappeared at stage 13. Using an eagle-kinesin-lacZ fusion gene as a reporter, the division, migration, and axonogenesis in eagle-positive cells and their derivatives were examined. At stage 14, several types of neural or glial cells were detected which include EG and EW interneurons joining to the anterior and posterior commissures, respectively. Lack of eagle expression caused altered axonogenesis in an appreciable fraction of eagle-Kinesin-LacZ-positive neurons. Some EG cells failed to acquire the neural fate or underwent an extremely delayed differentiation, while EW neurons produced neurites in abnormal directions, suggesting that eagle may play a critical role in development of the progeny of eagle-positive neuroblasts.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Tsuji, E. Hasegawa, and T. Isshiki Neuroblast entry into quiescence is regulated intrinsically by the combined action of spatial Hox proteins and temporal identity factors Development, December 1, 2008; 135(23): 3859 - 3869. [Abstract] [Full Text] [PDF]

				A. Rogulja-Ortmann, S. Renner, and G. M. Technau Antagonistic roles for Ultrabithorax and Antennapedia in regulating segment-specific apoptosis of differentiated motoneurons in the Drosophila embryonic central nervous system Development, October 15, 2008; 135(20): 3435 - 3445. [Abstract] [Full Text] [PDF]

				D. S. Garbe, M. O'Donnell, and G. J. Bashaw Cytoplasmic domain requirements for Frazzled-mediated attractive axon turning at the Drosophila midline Development, December 15, 2007; 134(24): 4325 - 4334. [Abstract] [Full Text] [PDF]

				D. S. Garbe and G. J. Bashaw Independent Functions of Slit-Robo Repulsion and Netrin-Frazzled Attraction Regulate Axon Crossing at the Midline in Drosophila J. Neurosci., March 28, 2007; 27(13): 3584 - 3592. [Abstract] [Full Text] [PDF]

				A. Rogulja-Ortmann, K. Luer, J. Seibert, C. Rickert, and G. M. Technau Programmed cell death in the embryonic central nervous system of Drosophila melanogaster Development, January 1, 2007; 134(1): 105 - 116. [Abstract] [Full Text] [PDF]

				S. J. Certel and S. Thor Specification of Drosophila motoneuron identity by the combinatorial action of POU and LIM-HD factors Development, November 1, 2004; 131(21): 5429 - 5439. [Abstract] [Full Text] [PDF]

				J. A. Couch, J. Chen, H. I. Rieff, E. M. Uri, and B. G. Condron robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation Development, March 1, 2004; 131(5): 997 - 1006. [Abstract] [Full Text] [PDF]

				A. A. Sullivan and C. S. Thummel Temporal Profiles of Nuclear Receptor Gene Expression Reveal Coordinate Transcriptional Responses during Drosophila Development Mol. Endocrinol., November 1, 2003; 17(11): 2125 - 2137. [Abstract] [Full Text] [PDF]

				M. J. Lundell, H.-K. Lee, E. Perez, and L. Chadwell The regulation of apoptosis by Numb/Notch signaling in the serotonin lineage of Drosophila Development, September 1, 2003; 130(17): 4109 - 4121. [Abstract] [Full Text] [PDF]

				M. Buescher, F. S. Hing, and W. Chia Formation of neuroblasts in the embryonic central nervous system of Drosophila melanogaster is controlled by SoxNeuro Development, March 11, 2003; 129(18): 4193 - 4203. [Abstract] [Full Text] [PDF]

				M. Georgiou and G. Tear Commissureless is required both in commissural neurones and midline cells for axon guidance across the midline Development, March 8, 2003; 129(12): 2947 - 2956. [Abstract] [Full Text] [PDF]

				T. Novotny, R. Eiselt, and J. Urban Hunchback is required for the specification of the early sublineage of neuroblast 7-3 in the Drosophila central nervous system Development, March 4, 2003; 129(4): 1027 - 1036. [Abstract] [Full Text] [PDF]

				K. Lunde, J. L. Trimble, A. Guichard, K. A. Guss, U. Nauber, and E. Bier Activation of the knirps locus links patterning to morphogenesis of the second wing vein in Drosophila Development, March 2, 2003; 130(2): 235 - 248. [Abstract] [Full Text] [PDF]

				M. R. Freeman and C. Q. Doe Asymmetric Prospero localization is required to generate mixed neuronal/glial lineages in the Drosophila CNS Development, October 15, 2001; 128(20): 4103 - 4112. [Abstract] [Full Text] [PDF]

				Y Akiyama-Oda, Y Hotta, S Tsukita, and H Oda Mechanism of glia-neuron cell-fate switch in the Drosophila thoracic neuroblast 6-4 lineage Development, January 8, 2000; 127(16): 3513 - 3522. [Abstract] [PDF]

				A Schmid, A Chiba, and C. Doe Clonal analysis of Drosophila embryonic neuroblasts: neural cell types, axon projections and muscle targets Development, January 11, 1999; 126(21): 4653 - 4689. [Abstract] [PDF]

				Y Akiyama-Oda, T Hosoya, and Y Hotta Asymmetric cell division of thoracic neuroblast 6-4 to bifurcate glial and neuronal lineage in Drosophila Development, January 5, 1999; 126(9): 1967 - 1974. [Abstract] [PDF]

				G Udolph, J Urban, G Rusing, K Luer, and G. Technau Differential effects of EGF receptor signalling on neuroblast lineages along the dorsoventral axis of the Drosophila CNS Development, January 9, 1998; 125(17): 3291 - 3299. [Abstract] [PDF]

				E. Dormand and A. Brand Runt determines cell fates in the Drosophila embryonic CNS Development, January 5, 1998; 125(9): 1659 - 1667. [Abstract] [PDF]

				M. Lundell and J Hirsh eagle is required for the specification of serotonin neurons and other neuroblast 7-3 progeny in the Drosophila CNS Development, January 2, 1998; 125(3): 463 - 472. [Abstract] [PDF]

				B. A. Hay, R. Maile, and G. M. Rubin P element insertion-dependent gene activation in the Drosophila eye PNAS, May 13, 1997; 94(10): 5195 - 5200. [Abstract] [Full Text] [PDF]

				T Isshiki, M Takeichi, and A Nose The role of the msh homeobox gene during Drosophila neurogenesis: implication for the dorsoventral specification of the neuroectoderm Development, January 8, 1997; 124(16): 3099 - 3109. [Abstract] [PDF]

				R Dittrich, T Bossing, A. Gould, G. Technau, and J Urban The differentiation of the serotonergic neurons in the Drosophila ventral nerve cord depends on the combined function of the zinc finger proteins Eagle and Huckebein Development, January 7, 1997; 124(13): 2515 - 2525. [Abstract] [PDF]

				J. McDonald and C. Doe Establishing neuroblast-specific gene expression in the Drosophila CNS: huckebein is activated by Wingless and Hedgehog and repressed by Engrailed and Gooseberry Development, January 3, 1997; 124(5): 1079 - 1087. [Abstract] [PDF]

				C. Sharpe and K Goldstone Retinoid receptors promote primary neurogenesis in Xenopus Development, January 1, 1997; 124(2): 515 - 523. [Abstract] [PDF]

				Y. Akiyama, T. Hosoya, A. M. Poole, and Y. Hotta The gcm-motif: A novel DNA-binding motif conserved in Drosophila and mammals PNAS, December 10, 1996; 93(25): 14912 - 14916. [Abstract] [Full Text] [PDF]

				M Matsuzaki and K Saigo hedgehog signaling independent of engrailed and wingless required for post-S1 neuroblast formation in Drosophila CNS Development, January 11, 1996; 122(11): 3567 - 3575. [Abstract] [PDF]