|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 2 579-588, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
ME Lieberfarb, T Chu, C Wreden, W Theurkauf, JP Gergen and S Strickland
Department of Pharmacology, University Medical Center at Stony Brook, NY 11794-8651, USA.
Translational recruitment of maternal mRNAs is an essential process in early metazoan development. To identify genes required for this regulatory pathway, we have examined a collection of Drosophila female-sterile mutants for defects in translation of maternal mRNAs. This strategy has revealed that maternal-effect mutations in the cortex and grauzone genes impair translational activation and cytoplasmic polyadenylation of bicoid and Toll mRNAs. Cortex embryos contain a bicoid mRNA indistinguishable in amount, localization, and structure from that in wild-type embryos. However, the bicoid mRNA in cortex embryos contains a shorter than normal polyadenosine (poly(A)) tail. Injection of polyadenylated bicoid mRNA into cortex embryos allows translation demonstrating that insufficient polyadenylation prevents endogenous bicoid mRNA translation. In contrast nanos mRNA, which is activated by a poly(A)-independent mechanism, is translated in cortex embryos, indicating that the block in maternal mRNA activation is specific to a class of mRNAs. Cortex embryos are fertilized, but arrest at the onset of embryogenesis. Characterization of grauzone mutations indicates that the phenotype of these embryos is similar to cortex. These results identify a fundamental pathway that serves a vital role in the initiation of development.
This article has been cited by other articles:
|
|
 |
|
  P. Benoit, C. Papin, J. E. Kwak, M. Wickens, and M. Simonelig PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila Development, June 1, 2008; 135(11): 1969 - 1979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swan and T. Schupbach The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II in Drosophila Development, March 1, 2007; 134(5): 891 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swan, G. Barcelo, and T. Schupbach Drosophila Cks30A interacts with Cdk1 to target Cyclin A for destruction in the female germline Development, August 15, 2005; 132(16): 3669 - 3678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sonneville and P. Gonczy zyg-11 and cul-2 regulate progression through meiosis II and polarity establishment in C. elegans Development, August 1, 2004; 131(15): 3527 - 3543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Tadros, S. A. Houston, A. Bashirullah, R. L. Cooperstock, J. L. Semotok, B. H. Reed, and H. D. Lipshitz Regulation of Maternal Transcript Destabilization During Egg Activation in Drosophila Genetics, July 1, 2003; 164(3): 989 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Castagnetti and A. Ephrussi Orb and a long poly(A) tail are required for efficient oskar translation at the posterior pole of the Drosophila oocyte Development, March 1, 2003; 130(5): 835 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gamberi, D. S. Peterson, L. He, and E. Gottlieb An anterior function for the Drosophila posterior determinant Pumilio Development, January 6, 2002; 129(11): 2699 - 2710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chagnovich and R. Lehmann Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo PNAS, September 13, 2001; (2001) 201284398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Harms, T. Chu, G. Henrion, and S. Strickland The Only Function of Grauzone Required for Drosophila Oocyte Meiosis Is Transcriptional Activation of the cortex Gene Genetics, August 1, 2000; 155(4): 1831 - 1839. [Abstract] [Full Text]
|
|
|
|
|
|
B Chen, E Harms, T Chu, G Henrion, and S Strickland Completion of meiosis in Drosophila oocytes requires transcriptional control by grauzone, a new zinc finger protein Development, January 3, 2000; 127(6): 1243 - 1251. [Abstract] [PDF]
|
|
|
|
 |
|
 J. D. Richter Cytoplasmic Polyadenylation in Development and Beyond Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 446 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Chen, T. Chu, E. Harms, J. P. Gergen, and S. Strickland Mapping of Drosophila Mutations Using Site-Specific Male Recombination Genetics, May 1, 1998; 149(1): 157 - 163. [Abstract] [Full Text]
|
|
|
|
|
|
J. Schisa and S Strickland Cytoplasmic polyadenylation of Toll mRNA is required for dorsal-ventral patterning in Drosophila embryogenesis Development, January 8, 1998; 125(15): 2995 - 3003. [Abstract] [PDF]
|
|
|
|
|
|
C Wreden, A. Verrotti, J. Schisa, M. Lieberfarb, and S Strickland Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA Development, January 8, 1997; 124(15): 3015 - 3023. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Page and T. Orr-Weaver The Drosophila genes grauzone and cortex are necessary for proper female meiosis J. Cell Sci., January 7, 1996; 109(7): 1707 - 1715. [Abstract] [PDF]
|
|
|
|
|
|
D. Chagnovich and R. Lehmann Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo PNAS, September 25, 2001; 98(20): 11359 - 11364. [Abstract] [Full Text] [PDF]
|
|
