|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 9 2769-2778, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
Y Saga, N Hata, S Kobayashi, T Magnuson, MF Seldin and MM Taketo
Banyu Tsukuba Research Institute (Merck), Ibaraki, Japan.
A subtractive hybridization strategy was used to isolate putative genes involved in the development of mouse primordial germ cells (PGC). Complimentary DNA was amplified on RNA isolated from the base of the allantois where PGC are located in the 7.5 days post coitum (dpc) mouse embryo. It was then subtracted by hybridization with cDNA amplified on RNA of the anterior region where PGC are absent. A novel gene thus isolated is designated as Mesp1 and encodes a possible transcription factor MesP1 containing a basic helix-loop-helix motif. Its earliest expression was observed at the onset of gastrulation, as early as 6.5 dpc, in the nascent mesodermal cells that first ingressed at the end of the primitive streak. These expressing cells in the lateral and extraembryonic mesoderm showed a wing-shaped distribution. Its initial expression was soon down-regulated at 7.5 dpc before the completion of gastrulation, except at the proximal end of the primitive streak which included the extraembryonic mesoderm and the base of allantois. At 8 dpc, the expression at the base of the allantois moved laterally. This distribution between 7.0 and 8.0 dpc was similar to that of PGC detected by the alkaline phosphatase activity. However, the expression of Mesp1 was down-regulated thereafter, when PGC entered in the migration stage. After birth, Mesp1 expression was detected only in mature testes, but in a different isoform from that expressed in the embryo. Mesp1 was mapped to the mid region of chromosome 7, near the mesodermal deficiency gene (mesd). However, a Southern hybridization study clearly showed that Mesp1 was distinctly different from mesd. The amino acid sequence and its expression pattern suggest that MesP1 plays an important role in the development of the nascent mesoderm including PGC.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Harada, A. Ogai, T. Takahashi, M. Kitakaze, H. Matsubara, and H. Oh Crossveinless-2 Controls Bone Morphogenetic Protein Signaling during Early Cardiomyocyte Differentiation in P19 Cells J. Biol. Chem., September 26, 2008; 283(39): 26705 - 26713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ueno, G. Weidinger, T. Osugi, A. D. Kohn, J. L. Golob, L. Pabon, H. Reinecke, R. T. Moon, and C. E. Murry From the Cover: Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells PNAS, June 5, 2007; 104(23): 9685 - 9690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Luo, Y. Xu, T. L. Hoffman, T. Zhang, T. Schilling, and T. D. Sargent Inca: a novel p21-activated kinase-associated protein required for cranial neural crest development Development, April 1, 2007; 134(7): 1279 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Davidson, W. Shi, J. Beh, L. Christiaen, and M. Levine FGF signaling delineates the cardiac progenitor field in the simple chordate, Ciona intestinalis Genes & Dev., October 1, 2006; 20(19): 2728 - 2738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, F. Cerrato, H. Xu, F. Vitelli, M. Morishima, J. Vincentz, Y. Furuta, L. Ma, J. F. Martin, A. Baldini, et al. Tbx1 expression in pharyngeal epithelia is necessary for pharyngeal arch artery development Development, December 1, 2005; 132(23): 5307 - 5315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Davidson, W. Shi, and M. Levine Uncoupling heart cell specification and migration in the simple chordate Ciona intestinalis Development, November 1, 2005; 132(21): 4811 - 4818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Behr, C. Heneweer, C. Viebahn, H.-W. Denker, and M. Thie Epithelial-Mesenchymal Transition in Colonies of Rhesus Monkey Embryonic Stem Cells: A Model for Processes Involved in Gastrulation Stem Cells, June 1, 2005; 23(6): 805 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takahashi, S. Kitajima, T. Inoue, J. Kanno, and Y. Saga Differential contributions of Mesp1 and Mesp2 to the epithelialization and rostro-caudal patterning of somites Development, February 15, 2005; 132(4): 787 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Piotrowska-Nitsche, A. Perea-Gomez, S. Haraguchi, and M. Zernicka-Goetz Four-cell stage mouse blastomeres have different developmental properties Development, February 1, 2005; 132(3): 479 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guillomot, A. Turbe, I. Hue, and J.-P. Renard Staging of ovine embryos and expression of the T-box genes Brachyury and Eomesodermin around gastrulation Reproduction, April 1, 2004; 127(4): 491 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Serth, K. Schuster-Gossler, R. Cordes, and A. Gossler Transcriptional oscillation of Lunatic fringe is essential for somitogenesis Genes & Dev., April 1, 2003; 17(7): 912 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nomura-Kitabayashi, Y. Takahashi, S. Kitajima, T. Inoue, H. Takeda, and Y. Saga Hypomorphic Mesp allele distinguishes establishment of rostrocaudal polarity and segment border formation in somitogenesis Development, March 7, 2003; 129(10): 2473 - 2481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mukhopadhyay, A. Teufel, T. Yamashita, A. D. Agulnick, L. Chen, K. M. Downs, A. Schindler, A. Grinberg, S.-P. Huang, D. Dorward, et al. Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation Development, February 1, 2003; 130(3): 495 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Hanna, R. K. Foreman, I. A. Tarasenko, D. S. Kessler, and P. A. Labosky Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo Genes & Dev., October 15, 2002; 16(20): 2650 - 2661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Dunwoodie, M. Clements, D. B. Sparrow, X. Sa, R. A. Conlon, and R. S. P. Beddington Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm Development, January 4, 2002; 129(7): 1795 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Perea-Gomez, K. Lawson, M Rhinn, L Zakin, P Brulet, S Mazan, and S. Ang Otx2 is required for visceral endoderm movement and for the restriction of posterior signals in the epiblast of the mouse embryo Development, January 3, 2001; 128(5): 753 - 765. [Abstract] [PDF]
|
|
|
|
|
|
T Ishikawa, Y Tamai, A. Zorn, H Yoshida, M. Seldin, S Nishikawa, and M. Taketo Mouse Wnt receptor gene Fzd5 is essential for yolk sac and placental angiogenesis Development, January 1, 2001; 128(1): 25 - 33. [Abstract] [PDF]
|
|
|
|
|
|
J. K. Yoon and B. Wold The bHLH regulator pMesogenin1 is required for maturation and segmentation of paraxial mesoderm Genes & Dev., December 15, 2000; 14(24): 3204 - 3214. [Abstract] [Full Text]
|
|
|
|
 |
|
 Tamai, Ishikawa, M. Bosl, Mori, Nozaki, Baribault, R. Oshima, and M. Taketo Cytokeratins 8 and 19 in the Mouse Placental Development J. Cell Biol., October 30, 2000; 151(3): 563 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Kitajima, A Takagi, T Inoue, and Y Saga MesP1 and MesP2 are essential for the development of cardiac mesoderm Development, January 8, 2000; 127(15): 3215 - 3226. [Abstract] [PDF]
|
|
|
|
|
|
A Sawada, A Fritz, Y Jiang, A Yamamoto, K Yamasu, A Kuroiwa, Y Saga, and H Takeda Zebrafish Mesp family genes, mesp-a and mesp-b are segmentally expressed in the presomitic mesoderm, and Mesp-b confers the anterior identity to the developing somites Development, January 4, 2000; 127(8): 1691 - 1702. [Abstract] [PDF]
|
|
|
|
|
|
L Durbin, P Sordino, A Barrios, M Gering, C Thisse, B Thisse, C Brennan, A Green, S Wilson, and N Holder Anteroposterior patterning is required within segments for somite boundary formation in developing zebrafish Development, January 4, 2000; 127(8): 1703 - 1713. [Abstract] [PDF]
|
|
|
|
|
|
M. Janatpour, M. McMaster, O Genbacev, Y Zhou, J Dong, J. Cross, M. Israel, and S. Fisher Id-2 regulates critical aspects of human cytotrophoblast differentiation, invasion and migration Development, January 2, 2000; 127(3): 549 - 558. [Abstract] [PDF]
|
|
|
|
|
|
W.-C. Jen, V. Gawantka, N. Pollet, C. Niehrs, and C. Kintner Periodic repression of Notch pathway genes governs the segmentation of Xenopus embryos Genes & Dev., June 1, 1999; 13(11): 1486 - 1499. [Abstract] [Full Text]
|
|
|
|
|
|
A Perea-Gomez, W Shawlot, H Sasaki, R. Behringer, and S Ang HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo Development, January 10, 1999; 126(20): 4499 - 4511. [Abstract] [PDF]
|
|
|
|
|
|
Y Saga, S Miyagawa-Tomita, A Takagi, S Kitajima, J. Miyazaki, and T Inoue MesP1 is expressed in the heart precursor cells and required for the formation of a single heart tube Development, January 8, 1999; 126(15): 3437 - 3447. [Abstract] [PDF]
|
|
|
|
|
|
D. Sparrow, W. Jen, S Kotecha, N Towers, C Kintner, and T. Mohun Thylacine 1 is expressed segmentally within the paraxial mesoderm of the Xenopus embryo and interacts with the Notch pathway Development, January 6, 1998; 125(11): 2041 - 2051. [Abstract] [PDF]
|
|
|
|
|
|
Y Saga, N Hata, H Koseki, and M M Taketo Mesp2: a novel mouse gene expressed in the presegmented mesoderm and essential for segmentation initiation. Genes & Dev., July 15, 1997; 11(14): 1827 - 1839. [Abstract] [PDF]
|
|
