|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 9 2903-2910, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
T Rankin, M Familari, E Lee, A Ginsberg, N Dwyer, J Blanchette-Mackie, J Drago, H Westphal and J Dean
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
Mammalian oocytes synthesize and secrete a zona pellucida that surrounds the growing oocytes, ovulated eggs and preimplantation embryos. The extracellular zona matrix is composed of three glycoproteins (ZP1, ZP2, ZP3) that are involved in folliculogenesis, species-specific fertilization, and passage of the early embryo down the oviduct. We have established a mouse line in which Zp3 has been inactivated by homologous recombination with an insertional mutation. Neither Zp3 transcripts nor ZP3 protein was detected in female mice homozygous for the mutation (Zp3-/-), whereas both ZP1 and ZP2 were present in mutant oocytes. Homozygous mutant Zp3-/- mice had follicles with germinal-vesicle-intact oocytes but that lacked a zona pellucida matrix and had a disorganized corona radiata. Although mutant oocytes underwent germinal vesicle breakdown (GVBD) prior to ovulation, the cumulus-oocyte complex was markedly disrupted and the oocytes were often separate from the cumulus cells. After hormone-induced ovulation, cumulus masses were present in the oviducts of homozygous mutant mice, but zona-free eggs were observed in only half of the females and, in these, less than 10% of the normal number [correction of mumber] of eggs were detected. No zona-free 2-cell embryos were recovered from homozygous mutant Zp3-/- female mice after mating with males proven to be fertile, and none became visibly pregnant or produced offspring. These results demonstrate that a genetic defect in a zona pellucida gene causes infertility and, given the conserved nature of the zona pellucida, a similar phenotype is expected in other mammals.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. A. Edson, A. K. Nagaraja, and M. M. Matzuk The Mammalian Ovary from Genesis to Revelation Endocr. Rev., October 1, 2009; 30(6): 624 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Fowler, S. Flannigan, A. Mathers, K. Gillanders, R. G. Lea, M. J. Wood, A. Maheshwari, S. Bhattacharya, E. S. R. Collie-Duguid, P. J. Baker, et al. Gene Expression Analysis of Human Fetal Ovarian Primordial Follicle Formation J. Clin. Endocrinol. Metab., April 1, 2009; 94(4): 1427 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Wassarman Zona Pellucida Glycoproteins J. Biol. Chem., September 5, 2008; 283(36): 24285 - 24289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Choi, D. J. Ballow, Y. Xin, and A. Rajkovic Lim Homeobox Gene, Lhx8, Is Essential for Mouse Oocyte Differentiation and Survival Biol Reprod, September 1, 2008; 79(3): 442 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C.N. Chiu, B. S.T. Wong, C.L. Lee, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung Native human zona pellucida glycoproteins: purification and binding properties Hum. Reprod., June 1, 2008; 23(6): 1385 - 1393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vallee, K. Aiba, Y. Piao, M.-F. Palin, M. S H Ko, and M.-A. Sirard Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species Reproduction, April 1, 2008; 135(4): 439 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ohsugi, P. Zheng, B. Baibakov, L. Li, and J. Dean Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos Development, January 15, 2008; 135(2): 259 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Gallardo, G. B. John, L. Shirley, C. M. Contreras, E. A. Akbay, J. M. Haynie, S. E. Ward, M. J. Shidler, and D. H. Castrillon Genomewide Discovery and Classification of Candidate Ovarian Fertility Genes in the Mouse Genetics, September 1, 2007; 177(1): 179 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A Swann, S. J B Cooper, and W. G Breed Molecular evolution of the carboxy terminal region of the zona pellucida 3 glycoprotein in murine rodents Reproduction, April 1, 2007; 133(4): 697 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Okumura, N. Aoki, C. Sato, D. Nadano, and T. Matsuda Heterocomplex Formation and Cell-Surface Accumulation of Hen's Serum Zona Pellucida B1 (ZPB1)with ZPC Expressed by a Mammalian Cell Line (COS-7): A Possible Initiating Step of Egg-Envelope Matrix Construction Biol Reprod, January 1, 2007; 76(1): 9 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hoodbhoy, M. Aviles, B. Baibakov, O. Epifano, M. Jimenez-Movilla, L. Gauthier, and J. Dean ZP2 and ZP3 Traffic Independently within Oocytes prior to Assembly into the Extracellular Zona Pellucida. Mol. Cell. Biol., November 1, 2006; 26(21): 7991 - 7998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hasegawa, N. Kanazawa, H. Sawai, S. Komori, and K. Koyama Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice. Reproduction, September 1, 2006; 132(3): 455 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.R. Barnett, C. Schilling, C.R. Greenfeld, D. Tomic, and J.A. Flaws Ovarian follicle development and transgenic mouse models Hum. Reprod. Update, September 1, 2006; 12(5): 537 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Ribas, J. E. Taylor, C. McCorquodale, A. C. Mauricio, M. Sousa, and I. Wilmut Effect of Zona Pellucida Removal on DNA Methylation in Early Mouse Embryos Biol Reprod, February 1, 2006; 74(2): 307 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Pangas and A. Rajkovic Transcriptional regulation of early oogenesis: in search of masters Hum. Reprod. Update, January 1, 2006; 12(1): 65 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wijgerde, M. Ooms, J. W. Hoogerbrugge, and J. A. Grootegoed Hedgehog Signaling in Mouse Ovary: Indian Hedgehog and Desert Hedgehog from Granulosa Cells Induce Target Gene Expression in Developing Theca Cells Endocrinology, August 1, 2005; 146(8): 3558 - 3566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mannikko, R.-M. Tormala, T. Tuuri, A. Haltia, H. Martikainen, L. Ala-Kokko, J.S. Tapanainen, and J.T. Lakkakorpi Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF Hum. Reprod., June 1, 2005; 20(6): 1578 - 1585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamatani, G. Falco, M. G. Carter, H. Akutsu, C. A. Stagg, A. A. Sharov, D. B. Dudekula, V. VanBuren, and M. S.H. Ko Age-associated alteration of gene expression patterns in mouse oocytes Hum. Mol. Genet., October 1, 2004; 13(19): 2263 - 2278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. V. Ramos, D. J. Stumpo, E. A. Kennington, R. S. Phillips, C. B. Bock, F. Ribeiro-Neto, and P. J. Blackshear The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development Development, October 1, 2004; 131(19): 4883 - 4893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hoodbhoy and J. Dean Insights into the molecular basis of sperm-egg recognition in mammals Reproduction, April 1, 2004; 127(4): 417 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dell, S. Chalabi, R. L. Easton, S. M. Haslam, M. Sutton-Smith, M. S. Patankar, F. Lattanzio, M. Panico, H. R. Morris, and G. F. Clark Murine and human zona pellucida 3 derived from mouse eggs express identical O-glycans PNAS, December 23, 2003; 100(26): 15631 - 15636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhao, L. Gold, H. Dorward, L.-f. Liang, T. Hoodbhoy, E. Boja, H. M. Fales, and J. Dean Mutation of a Conserved Hydrophobic Patch Prevents Incorporation of ZP3 into the Zona Pellucida Surrounding Mouse Eggs Mol. Cell. Biol., December 15, 2003; 23(24): 8982 - 8991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Boja, T. Hoodbhoy, H. M. Fales, and J. Dean Structural Characterization of Native Mouse Zona Pellucida Proteins Using Mass Spectrometry J. Biol. Chem., September 5, 2003; 278(36): 34189 - 34202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Primakoff and D. G. Myles Penetration, Adhesion, and Fusion in Mammalian Sperm-Egg Interaction Science, June 21, 2002; 296(5576): 2183 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhao, L. Gold, A. M. Ginsberg, L.-F. Liang, and J. Dean Conserved Furin Cleavage Site Not Essential for Secretion and Integration of ZP3 into the Extracellular Egg Coat of Transgenic Mice Mol. Cell. Biol., May 1, 2002; 22(9): 3111 - 3120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. El-Mestrah, P. E. Castle, G. Borossa, and F. W.K. Kan Subcellular Distribution of ZP1, ZP2, and ZP3 Glycoproteins During Folliculogenesis and Demonstration of Their Topographical Disposition Within the Zona Matrix of Mouse Ovarian Oocytes Biol Reprod, April 1, 2002; 66(4): 866 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kiefer and P. Saling Proteolytic Processing of Human Zona Pellucida Proteins Biol Reprod, February 1, 2002; 66(2): 407 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takasaki, T. Rankin, and J. Dean Normal Gonadal Development in Mice Lacking GPBOX, a Homeobox Protein Expressed in Germ Cells at the Onset of Sexual Dimorphism Mol. Cell. Biol., December 1, 2001; 21(23): 8197 - 8202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Rankin, M O'Brien, E Lee, K Wigglesworth, J Eppig, and J Dean Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development Development, January 4, 2001; 128(7): 1119 - 1126. [Abstract] [PDF]
|
|
|
|
 |
|
 B. S. Dunbar, S. Prasad, C. Carino, and S. M. Skinner The Ovary as an Immune Target Reproductive Sciences, January 1, 2001; 8(1_suppl): S43 - S48. [Abstract] [PDF]
|
|
|
|
|
|
K. A. Eidne, C. C. Henery, and R. J. Aitken Selection of Peptides Targeting the Human Sperm Surface Using Random Peptide Phage Display Identify Ligands Homologous to ZP3 Biol Reprod, November 1, 2000; 63(5): 1396 - 1402. [Abstract] [Full Text]
|
|
|
|
|
|
J. P. Evans Getting Sperm and Egg Together: Things Conserved and Things Diverged Biol Reprod, August 1, 2000; 63(2): 355b - 360. [Abstract] [Full Text]
|
|
|
|
|
|
F. Le Naour, E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix Severely Reduced Female Fertility in CD9-Deficient Mice Science, January 14, 2000; 287(5451): 319 - 321. [Abstract] [Full Text]
|
|
|
|
|
|
S. Soyal, A Amleh, and J Dean FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation Development, January 11, 2000; 127(21): 4645 - 4654. [Abstract] [PDF]
|
|
|
|
|
|
S. Greenhouse, P. E. Castle, and J. Dean Antibodies to human ZP3 induce reversible contraception in transgenic mice with `humanized' zonae pellucidae Hum. Reprod., March 1, 1999; 14(3): 593 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hinsch, S. Oehninger, W.-B. Schill, and K.-D. Hinsch Species specificity of human and murine anti-ZP3 synthetic peptide antisera and use of the antibodies for localization and identification of ZP3 or ZPC domains of functional significance Hum. Reprod., February 1, 1999; 14(2): 419 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Rankin, P Talbot, E Lee, and J Dean Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss Development, January 9, 1999; 126(17): 3847 - 3855. [Abstract] [PDF]
|
|
|
|
|
|
Y. Sidis,, T. Fujiwara,, L. Leykin,, K. Isaacson,, T. Toth,, and A. L. Schneyer Characterization of Inhibin/Activin Subunit, Activin Receptor, and Follistatin Messenger Ribonucleic Acid in Human and Mouse Oocytes: Evidence for Activin's Paracrine Signaling from Granulosa Cells to Oocytes Biol Reprod, October 1, 1998; 59(4): 807 - 812. [Abstract] [Full Text]
|
|
|
|
|
|
T. Rankin, Z. Tong, P. Castle, E Lee, R Gore-Langton, L. Nelson, and J Dean Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding Development, January 7, 1998; 125(13): 2415 - 2424. [Abstract] [PDF]
|
|
|
|
|
|
L Liang, S. Soyal, and J Dean FIGalpha, a germ cell specific transcription factor involved in the coordinate expression of the zona pellucida genes Development, January 12, 1997; 124(24): 4939 - 4947. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Qi, Z. Williams, and P. M. Wassarman Secretion and Assembly of Zona Pellucida Glycoproteins by Growing Mouse Oocytes Microinjected with Epitope-tagged cDNAs for mZP2 and mZP3 Mol. Biol. Cell, February 1, 2002; 13(2): 530 - 541. [Abstract] [Full Text] [PDF]
|
|
