|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 123, Issue 1 415-425, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
H Baier, S Klostermann, T Trowe, RO Karlstrom, C Nusslein-Volhard and F Bonhoeffer
Abteilung Physikalische Biologie, Max-Planck-Institut fur Entwicklungsbiologie, Tubingen, Germany. baier@jeeves.ucsd.edu
A systematic search for mutations affecting the retinotectal projection in zebrafish larvae was performed, as part of the large-scale Tubingen screen for homozygous diploid mutants in embryonic development. 2,746 inbred lines (F2 families) from males mutagenized with ethylnitroso urea were screened. In wild-type larvae, developing retinal axons travel along a stereotyped route to the contralateral optic tectum. Here, their terminals form a highly ordered retinotopic map. To detect deviations from this pattern, an axon tracing assay was developed that permits screening of large numbers of mutagenized fish. Two fluorescent tracer dyes (DiI and DiO) were injected at opposite poles of the eyes of day-5 aldehyde-fixed larvae. 12 hours later, retinal axons were labelled over their entire length, and could be observed through the intact skin. The assay procedure (aldehyde fixation, mounting, injection of dyes, microscopic analysis) took about 1 minute per fish. In total, 125,000 individual fish larvae were processed. During the screen, 114 mutations in approx. 35 genes were discovered. For the mutants subjected to complementation testing, the number of alleles per locus ranges from 1 to 15. The mutations affect distinct steps in the retinotectal pathway, from pathfinding between eye and tectum to map formation along the dorsal-ventral and the anterior-posterior axis of the tectum. Mutations that disturb axon pathfinding to the tectum for the most part do not disrupt retinotopic mapping, and vice versa. The majority of the mutants display associated defects in other tissues and die before day 10. These mutants provide new tools for studying the formation of neuronal maps. The results of this screen show that a large-scale genetic approach can be applied to relatively late and circumscribed developmental processes in the vertebrate brain.
This article has been cited by other articles:
|
|
 |
|
  K. Saitoh, A. Menard, and S. Grillner Tectal Control of Locomotion, Steering, and Eye Movements in Lamprey J Neurophysiol, April 1, 2007; 97(4): 3093 - 3108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Chalasani, A. Sabol, H. Xu, M. A. Gyda, K. Rasband, M. Granato, C.-B. Chien, and J. A. Raper Stromal Cell-Derived Factor-1 Antagonizes Slit/Robo Signaling In Vivo J. Neurosci., January 31, 2007; 27(5): 973 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Xiao, T. Roeser, W. Staub, and H. Baier A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection Development, July 1, 2005; 132(13): 2955 - 2967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D'Souza, M. Hendricks, S. Le Guyader, S. Subburaju, B. Grunewald, K. Scholich, and S. Jesuthasan Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc) Development, January 15, 2005; 132(2): 247 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Johnson and D. Van Vactor Receptor Protein Tyrosine Phosphatases in Nervous System Development Physiol Rev, January 1, 2003; 83(1): 1 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Thisse and L. I. Zon Organogenesis--Heart and Blood Formation from the Zebrafish Point of View Science, January 18, 2002; 295(5554): 457 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Briggs The zebrafish: a new model organism for integrative physiology Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R3 - R9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gnuegge, S. Schmid, and S. C. F. Neuhauss Analysis of the Activity-Deprived Zebrafish Mutant macho Reveals an Essential Requirement of Neuronal Activity for the Development of a Fine-Grained Visuotopic Map J. Neurosci., May 15, 2001; 21(10): 3542 - 3548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fricke, J.-S. Lee, S. Geiger-Rudolph, F. Bonhoeffer, and C.-B. Chien astray, a Zebrafish roundabout Homolog Required for Retinal Axon Guidance Science, April 20, 2001; 292(5516): 507 - 510. [Abstract] [Full Text]
|
|
|
|
|
|
L. Li and J. E. Dowling Disruption of the Olfactoretinal Centrifugal Pathway May Relate to the Visual System Defect in night blindness b Mutant Zebrafish J. Neurosci., March 1, 2000; 20(5): 1883 - 1892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Amsterdam, S. Burgess, G. Golling, W. Chen, Z. Sun, K. Townsend, S. Farrington, M. Haldi, and N. Hopkins A large-scale insertional mutagenesis screen in zebrafish Genes & Dev., October 15, 1999; 13(20): 2713 - 2724. [Abstract] [Full Text]
|
|
|
|
|
|
S. C. F. Neuhauss, O. Biehlmaier, M. W. Seeliger, T. Das, K. Kohler, W. A. Harris, and H. Baier Genetic Disorders of Vision Revealed by a Behavioral Screen of 400 Essential Loci in Zebrafish J. Neurosci., October 1, 1999; 19(19): 8603 - 8615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Anderson and B Key Novel guidance cues during neuronal pathfinding in the early scaffold of axon tracts in the rostral brain Development, January 5, 1999; 126(9): 1859 - 1868. [Abstract] [PDF]
|
|
|
|
|
|
J Malicki and W Driever oko meduzy mutations affect neuronal patterning in the zebrafish retina and reveal cell-cell interactions of the retinal neuroepithelial sheet Development, January 3, 1999; 126(6): 1235 - 1246. [Abstract] [PDF]
|
|
|
|
|
|
A. B. Ribera and C. Nusslein-Volhard Zebrafish Touch-Insensitive Mutants Reveal an Essential Role for the Developmental Regulation of Sodium Current J. Neurosci., November 15, 1998; 18(22): 9181 - 9191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Macdonald, J Scholes, U Strahle, C Brennan, N Holder, M Brand, and S. Wilson The Pax protein Noi is required for commissural axon pathway formation in the rostral forebrain Development, January 6, 1997; 124(12): 2397 - 2408. [Abstract] [PDF]
|
|
|
|
|
|
P Haffter, M Granato, M Brand, M. Mullins, M Hammerschmidt, D. Kane, J Odenthal, F. van Eeden, Y. Jiang, C. Heisenberg, et al. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 1 - 36. [Abstract] [PDF]
|
|
|
|
|
|
M Brand, C. Heisenberg, R. Warga, F Pelegri, R. Karlstrom, D Beuchle, A Picker, Y. Jiang, M Furutani-Seiki, F. van Eeden, et al. Mutations affecting development of the midline and general body shape during zebrafish embryogenesis Development, January 12, 1996; 123(1): 129 - 142. [Abstract] [PDF]
|
|
|
|
|
|
F. van Eeden, M Granato, U Schach, M Brand, M Furutani-Seiki, P Haffter, M Hammerschmidt, C. Heisenberg, Y. Jiang, D. Kane, et al. Mutations affecting somite formation and patterning in the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 153 - 164. [Abstract] [PDF]
|
|
|
|
|
|
C. Heisenberg, M Brand, Y. Jiang, R. Warga, D Beuchle, F. van Eeden, M Furutani-Seiki, M Granato, P Haffter, M Hammerschmidt, et al. Genes involved in forebrain development in the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 191 - 203. [Abstract] [PDF]
|
|
|
|
|
|
Y. Jiang, M Brand, C. Heisenberg, D Beuchle, M Furutani-Seiki, R. Kelsh, R. Warga, M Granato, P Haffter, M Hammerschmidt, et al. Mutations affecting neurogenesis and brain morphology in the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 205 - 216. [Abstract] [PDF]
|
|
|
|
|
|
F. van Eeden, M Granato, U Schach, M Brand, M Furutani-Seiki, P Haffter, M Hammerschmidt, C. Heisenberg, Y. Jiang, D. Kane, et al. Genetic analysis of fin formation in the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 255 - 262. [Abstract] [PDF]
|
|
|
|
|
|
R. Kelsh, M Brand, Y. Jiang, C. Heisenberg, S Lin, P Haffter, J Odenthal, M. Mullins, F. van Eeden, M Furutani-Seiki, et al. Zebrafish pigmentation mutations and the processes of neural crest development Development, January 12, 1996; 123(1): 369 - 389. [Abstract] [PDF]
|
|
|
|
|
|
J Odenthal, K Rossnagel, P Haffter, R. Kelsh, E Vogelsang, M Brand, F. van Eeden, M Furutani-Seiki, M Granato, M Hammerschmidt, et al. Mutations affecting xanthophore pigmentation in the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 391 - 398. [Abstract] [PDF]
|
|
|
|
|
|
H Baier, S Klostermann, T Trowe, R. Karlstrom, C Nusslein-Volhard, and F Bonhoeffer Genetic dissection of the retinotectal projection Development, January 12, 1996; 123(1): 415 - 425. [Abstract] [PDF]
|
|
|
|
|
|
T Trowe, S Klostermann, H Baier, M Granato, A. Crawford, B Grunewald, H Hoffmann, R. Karlstrom, S. Meyer, B Muller, et al. Mutations disrupting the ordering and topographic mapping of axons in the retinotectal projection of the zebrafish, Danio rerio Development, January 12, 1996; 123(1): 439 - 450. [Abstract] [PDF]
|
|
