Development, Vol 124, Issue 1 125-132, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Specification of the embryonic limb primordium by graded activity of Decapentaplegic

S Goto and S Hayashi
Genetic Stock Research Center, National Institute of Genetics, Mishima, Japan.

Two thoracic limbs of Drosophila, the leg and the wing, originate from a common cluster of cells that include the source of two secreted signaling molecules, Decapentaplegic and Wingless. We show that Wingless, but not Decapentaplegic, is responsible for initial specification of the limb primordia with a distal identity. Limb formation is restricted to the lateral position of the embryo by negative control of the early function of Decapentaplegic and the EGF receptor homolog that determine the global dorsoventral pattern. Late function of Decapentaplegic locally determines two additional cell identities in a dosage dependent manner. Loss of Decapentaplegic activity results in a deletion of the proximal structures of the limb, which is in contrast to the consequence of decapentaplegic mutations in the imaginal disc, which cause a deletion of distal structures. The results indicate that the limb pattern elements are added in a distal to proximal direction in the embryo, which is opposite to what is happening in the growing imaginal disc. We propose that Wingless and Decapentaplegic act sequentially to initiate the proximodistal axis.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. Perez-Garijo, E. Shlevkov, and G. Morata The role of Dpp and Wg in compensatory proliferation and in the formation of hyperplastic overgrowths caused by apoptotic cells in the Drosophila wing disc Development, April 1, 2009; 136(7): 1169 - 1177. [Abstract] [Full Text] [PDF]

				D. J. McKay, C. Estella, and R. S. Mann The origins of the Drosophila leg revealed by the cis-regulatory architecture of the Distalless gene Development, January 1, 2009; 136(1): 61 - 71. [Abstract] [Full Text] [PDF]

				J. Culi, P. Aroca, J. Modolell, and R. S. Mann jing Is Required for Wing Development and to Establish the Proximo-Distal Axis of the Leg in Drosophila melanogaster Genetics, May 1, 2006; 173(1): 255 - 266. [Abstract] [Full Text] [PDF]

				E. L. Jockusch and K. A. Ober Hypothesis Testing in Evolutionary Developmental Biology: A Case Study from Insect Wings J. Hered., September 1, 2004; 95(5): 382 - 396. [Abstract] [Full Text] [PDF]

				C. Estella, G. Rieckhof, M. Calleja, and G. Morata The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila Development, December 15, 2003; 130(24): 5929 - 5941. [Abstract] [Full Text] [PDF]

				M. Rozowski and M. Akam Hox gene control of segment-specific bristle patterns in Drosophila Genes & Dev., May 1, 2002; 16(9): 1150 - 1162. [Abstract] [Full Text] [PDF]

				G. Panganiban and J. L. R. Rubenstein Developmental functions of the Distal-less/Dlx homeobox genes Development, January 10, 2002; 129(19): 4371 - 4386. [Abstract] [Full Text] [PDF]

				J Torres-Vazquez, S Park, R Warrior, and K Arora The transcription factor Schnurri plays a dual role in mediating Dpp signaling during embryogenesis Development, January 5, 2001; 128(9): 1657 - 1670. [Abstract] [PDF]

				N Niwa, Y Inoue, A Nozawa, M Saito, Y Misumi, H Ohuchi, H Yoshioka, and S Noji Correlation of diversity of leg morphology in Gryllus bimaculatus (cricket) with divergence in dpp expression pattern during leg development Development, January 10, 2000; 127(20): 4373 - 4381. [Abstract] [PDF]

				K Kubota, S Goto, K Eto, and S Hayashi EGF receptor attenuates Dpp signaling and helps to distinguish the wing and leg cell fates in Drosophila Development, January 9, 2000; 127(17): 3769 - 3776. [Abstract] [PDF]

				T Suzuki and K Saigo Transcriptional regulation of atonal required for Drosophila larval eye development by concerted action of eyes absent, sine oculis and hedgehog signaling independent of fused kinase and cubitus interruptus Development, January 4, 2000; 127(7): 1531 - 1540. [Abstract] [PDF]

				E. Jockusch, C Nulsen, S. Newfeld, and L. Nagy Leg development in flies versus grasshoppers: differences in dpp expression do not lead to differences in the expression of downstream components of the leg patterning pathway Development, January 4, 2000; 127(8): 1617 - 1626. [Abstract] [PDF]

				P. Dong, J Chu, and G Panganiban Coexpression of the homeobox genes Distal-less and homothorax determines Drosophila antennal identity Development, January 1, 2000; 127(2): 209 - 216. [Abstract] [PDF]

				A. C. Spradling, D. Stern, A. Beaton, E. J. Rhem, T. Laverty, N. Mozden, S. Misra, and G. M. Rubin The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-Element Insertions Mutating 25% of Vital Drosophila Genes Genetics, September 1, 1999; 153(1): 135 - 177. [Abstract] [Full Text]

				S Goto and S Hayashi Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning Development, January 8, 1999; 126(15): 3407 - 3413. [Abstract] [PDF]

				G Campbell and A Tomlinson The roles of the homeobox genes aristaless and Distal-less in patterning the legs and wings of Drosophila Development, January 11, 1998; 125(22): 4483 - 4493. [Abstract] [PDF]

				L Maves and G Schubiger A molecular basis for transdetermination in Drosophila imaginal discs: interactions between wingless and decapentaplegic signaling Development, January 1, 1998; 125(1): 115 - 124. [Abstract] [PDF]

				P Wappner, L Gabay, and B. Shilo Interactions between the EGF receptor and DPP pathways establish distinct cell fates in the tracheal placodes Development, January 11, 1997; 124(22): 4707 - 4716. [Abstract] [PDF]

				J. Kim, J. Magee, and S.B. Carroll Intercompartmental Signaling and the Regulation of vestigial Expression at the Dorsoventral Boundary of the Developing Drosophila Wing Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 283 - 291. [Abstract] [PDF]