|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 10 1985-1997, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
GS Mastick, NM Davis, GL Andrew and SS Easter
Department of Biology, University of Michigan, Ann Arbor 48109, USA. grants@umich.edu
The Pax-6 gene encodes a transcription factor that is expressed in regionally restricted patterns in the developing brain and eye. Here we describe Pax-6 expression in the early forebrain (prosencephalon) on embryonic day 9.5 (E9.5) to E10.5 using both whole-mount in situ hybridization and antibody labeling. We find close correlations between Pax-6+ domains and initial neural patterning, and identify corresponding defects in embryos homozygous for the Pax-6 allele, Small eye (Sey). Pax-6 expression defines the prosencephalon-mesencephalon boundary, and mutant embryos lack this morphological boundary. Markers of the caudal prosencephalon are lost (Pax-6, Lim-1, Gsh-1) and a marker for mesencephalon is expanded rostrally into the prosencephalon (Dbx). We conclude that the caudal prosencephalon (prosomere 1) is at least partially transformed to a mesencephalic fate. This transformation results in a specific deficit of posterior commissure axons. Sey/Sey embryos also exhibit an axon pathfinding defect specific to the first longitudinal tract in the prosencephalon (tpoc, tract of the postoptic commissure). In wild type, tpoc axons fan out upon coming in contact with a superficial patch of Pax-6+ neuron cell bodies. In the mutant, the tpoc axons have normal initial projections, but make dramatic errors where they contact the neuron cell bodies, and fail to pioneer this first tract. Thus Pax-6 is required for local navigational information used by axons passing through its domain of expression. We conclude that Pax-6 plays multiple roles in forebrain patterning, including boundary formation, regional patterning, neuron specification and axon guidance.
This article has been cited by other articles:
|
|
 |
|
  P. P. Gopal and J. A. Golden Pax6-/- Mice Have a Cell Nonautonomous Defect in Nonradial Interneuron Migration Cereb Cortex, April 1, 2008; 18(4): 752 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Manuel, P. A. Georgala, C. B. Carr, S. Chanas, D. A. Kleinjan, B. Martynoga, J. O. Mason, M. Molinek, J. Pinson, T. Pratt, et al. Controlled overexpression of Pax6 in vivo negatively autoregulates the Pax6 locus, causing cell-autonomous defects of late cortical progenitor proliferation with little effect on cortical arealization Development, February 1, 2007; 134(3): 545 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hirata, M. Nakazawa, O. Muraoka, R. Nakayama, Y. Suda, and M. Hibi Zinc-finger genes Fez and Fez-like function in the establishment of diencephalon subdivisions Development, October 15, 2006; 133(20): 3993 - 4004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Fotaki, T. Yu, P. A. Zaki, J. O. Mason, and D. J. Price Abnormal Positioning of Diencephalic Cell Types in Neocortical Tissue in the Dorsal Telencephalon of Mice Lacking Functional Gli3 J. Neurosci., September 6, 2006; 26(36): 9282 - 9292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Garelli, N. P. Rotstein, and L. E. Politi Docosahexaenoic Acid Promotes Photoreceptor Differentiation without Altering Crx Expression. Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 3017 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nomura, J. Holmberg, J. Frisen, and N. Osumi Pax6-dependent boundary defines alignment of migrating olfactory cortex neurons via the repulsive activity of ephrin A5 Development, April 1, 2006; 133(7): 1335 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. N. Cinar and A. D. Chisholm Genetic Analysis of the Caenorhabditis elegans pax-6 Locus: Roles of Paired Domain-Containing and Nonpaired Domain-Containing Isoforms Genetics, November 1, 2004; 168(3): 1307 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pratt, N. M. M.-L. Tian, T. I. Simpson, J. O. Mason, and D. J. Price The winged helix transcription factor Foxg1 facilitates retinal ganglion cell axon crossing of the ventral midline in the mouse Development, August 1, 2004; 131(15): 3773 - 3784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hirose, Z. M. Varga, H. Kondoh, and M. Furutani-Seiki Single cell lineage and regionalization of cell populations during Medaka neurulation Development, June 1, 2004; 131(11): 2553 - 2563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Andrews and G. S. Mastick R-Cadherin Is a Pax6-Regulated, Growth-Promoting Cue for Pioneer Axons J. Neurosci., October 29, 2003; 23(30): 9873 - 9880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Scholpp, C. Lohs, and M. Brand Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon Development, October 15, 2003; 130(20): 4881 - 4893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tallafuss and L. Bally-Cuif Tracing of her5 progeny in zebrafish transgenics reveals the dynamics of midbrain-hindbrain neurogenesis and maintenance Development, September 15, 2003; 130(18): 4307 - 4323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bach, Y. Lallemand, M.-A. Nicola, C. Ramos, L. Mathis, M. Maufras, and B. Robert Msx1 is required for dorsal diencephalon patterning Development, September 1, 2003; 130(17): 4025 - 4036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Baumer, T. Marquardt, A. Stoykova, D. Spieler, D. Treichel, R. Ashery-Padan, and P. Gruss Retinal pigmented epithelium determination requires the redundant activities of Pax2 and Pax6 Development, July 1, 2003; 130(13): 2903 - 2915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Molnar, S. Higashi, and G. Lopez-Bendito Choreography of Early Thalamocortical Development Cereb Cortex, June 1, 2003; 13(6): 661 - 669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Davis, M. K. Duncan, W. G. Robison Jr, and J. Piatigorsky Requirement for Pax6 in corneal morphogenesis: a role in adhesion J. Cell Sci., June 1, 2003; 116(11): 2157 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishibashi and A. P. McMahon A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo Development, March 12, 2003; 129(20): 4807 - 4819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Burgess, G. Reim, W. Chen, N. Hopkins, and M. Brand The zebrafish spiel-ohne-grenzen (spg) gene encodes the POU domain protein Pou2 related to mammalian Oct4 and is essential for formation of the midbrain and hindbrain, and for pre-gastrula morphogenesis Development, March 4, 2003; 129(4): 905 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Estivill-Torrus, H. Pearson, V. van Heyningen, D. J. Price, and P. Rashbass Pax6 is required to regulate the cell cycle and the rate of progression from symmetrical to asymmetrical division in mammalian cortical progenitors Development, March 3, 2003; 129(2): 455 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pratt, J. C. Quinn, T. I. Simpson, J. D. West, J. O. Mason, and D. J. Price Disruption of Early Events in Thalamocortical Tract Formation in Mice Lacking the Transcription Factors Pax6 or Foxg1 J. Neurosci., October 1, 2002; 22(19): 8523 - 8531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Chauhan, W. Zhang, K. Cveklova, M. Kantorow, and A. Cvekl Identification of Differentially Expressed Genes in Mouse Pax6 Heterozygous Lenses Invest. Ophthalmol. Vis. Sci., June 1, 2002; 43(6): 1884 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Chauhan, N. A. Reed, W. Zhang, M. K. Duncan, M. W. Kilimann, and A. Cvekl Identification of Genes Downstream of Pax6 in the Mouse Lens Using cDNA Microarrays J. Biol. Chem., March 22, 2002; 277(13): 11539 - 11548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jones, G. Lopez-Bendito, P. Gruss, A. Stoykova, and Z. Molnar Pax6 is required for the normal development of the forebrain axonal connections Development, January 11, 2002; 129(21): 5041 - 5052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Matsunaga, I. Araki, and H. Nakamura Role of Pax3/7 in the tectum regionalization Development, October 15, 2001; 128(20): 4069 - 4077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamasaki, K. Kawaji, K. Ono, H. Bito, T. Hirano, N. Osumi, and M. Kengaku Pax6 regulates granule cell polarization during parallel fiber formation in the developing cerebellum Development, August 15, 2001; 128(16): 3133 - 3144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Rasmussen, M. A. Deardorff, C. Tan, M. S. Rao, P. S. Klein, and M. L. Vetter Regulation of eye development by frizzled signaling in Xenopus PNAS, March 27, 2001; 98(7): 3861 - 3866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Yun, S Potter, and J. Rubenstein Gsh2 and Pax6 play complementary roles in dorsoventral patterning of the mammalian telencephalon Development, January 1, 2001; 128(2): 193 - 205. [Abstract] [PDF]
|
|
|
|
|
|
A. Stoykova, D. Treichel, M. Hallonet, and P. Gruss Pax6 Modulates the Dorsoventral Patterning of the Mammalian Telencephalon J. Neurosci., November 1, 2000; 20(21): 8042 - 8050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tomioka, N. Osumi, Y. Sato, T. Inoue, S. Nakamura, H. Fujisawa, and T. Hirata Neocortical Origin and Tangential Migration of Guidepost Neurons in the Lateral Olfactory Tract J. Neurosci., August 1, 2000; 20(15): 5802 - 5812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bulfone, E. Menguzzato, V. Broccoli, A. Marchitiello, C. Gattuso, M. Mariani, G. G. Consalez, S. Martinez, A. Ballabio, and S. Banfi Barhl1, a gene belonging to a new subfamily of mammalian homeobox genes, is expressed in migrating neurons of the CNS Hum. Mol. Genet., May 22, 2000; 9(9): 1443 - 1452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Pratt, T Vitalis, N Warren, J. Edgar, J. Mason, and D. Price A role for Pax6 in the normal development of dorsal thalamus and its cortical connections Development, January 12, 2000; 127(23): 5167 - 5178. [Abstract] [PDF]
|
|
|
|
|
|
A Noveen, A Daniel, and V Hartenstein Early development of the Drosophila mushroom body: the roles of eyeless and dachshund Development, January 8, 2000; 127(16): 3475 - 3488. [Abstract] [PDF]
|
|
|
|
|
|
E Matsunaga, I Araki, and H Nakamura Pax6 defines the di-mesencephalic boundary by repressing En1 and Pax2 Development, January 6, 2000; 127(11): 2357 - 2365. [Abstract] [PDF]
|
|
|
|
|
|
K. Chung, J. Taylor, D. Shum, and S. Chan Axon routing at the optic chiasm after enzymatic removal of chondroitin sulfate in mouse embryos Development, January 6, 2000; 127(12): 2673 - 2683. [Abstract] [PDF]
|
|
|
|
|
|
J. Collinson, R. Hill, and J. West Different roles for Pax6 in the optic vesicle and facial epithelium mediate early morphogenesis of the murine eye Development, January 3, 2000; 127(5): 945 - 956. [Abstract] [PDF]
|
|
|
|
|
|
G Alvarez-Bolado, X Zhou, A. Voss, T Thomas, and P Gruss Winged helix transcription factor Foxb1 is essential for access of mammillothalamic axons to the thalamus Development, January 3, 2000; 127(5): 1029 - 1038. [Abstract] [PDF]
|
|
|
|
|
|
C. Kioussi, S. O'Connell, L. St-Onge, M. Treier, A. S. Gleiberman, P. Gruss, and M. G. Rosenfeld Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development PNAS, December 7, 1999; 96(25): 14378 - 14382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Warren, D. Caric, T. Pratt, J. A. Clausen, P. Asavaritikrai, J. O. Mason, R. E. Hill, and D. J. Price The Transcription Factor, Pax6, is Required for Cell Proliferation and Differentiation in the Developing Cerebral Cortex Cereb Cortex, September 1, 1999; 9(6): 627 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Engelkamp, P Rashbass, A Seawright, and V van Heyningen Role of Pax6 in development of the cerebellar system Development, January 8, 1999; 126(16): 3585 - 3596. [Abstract] [PDF]
|
|
|
|
|
|
R Tuttle, Y Nakagawa, J. Johnson, and D. O'Leary Defects in thalamocortical axon pathfinding correlate with altered cell domains in Mash-1-deficient mice Development, January 5, 1999; 126(9): 1903 - 1916. [Abstract] [PDF]
|
|
|
|
 |
|
 J. B. Weiss, T. Von Ohlen, D. M. Mellerick, G. Dressler, C. Q. Doe, and M. P. Scott Dorsoventral patterning in the Drosophila central nervous system: the intermediate neuroblasts defective homeobox gene specifies intermediate column identity Genes & Dev., November 15, 1998; 12(22): 3591 - 3602. [Abstract] [Full Text]
|
|
|
|
|
|
S. Alcantara, M. Ruiz, G. D'Arcangelo, F. Ezan, L. de Lecea, T. Curran, C. Sotelo, and E. Soriano Regional and Cellular Patterns of reelin mRNA Expression in the Forebrain of the Developing and Adult Mouse J. Neurosci., October 1, 1998; 18(19): 7779 - 7799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Brown, S Kanekar, M. Vetter, P. Tucker, D. Gemza, and T Glaser Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis Development, January 12, 1998; 125(23): 4821 - 4833. [Abstract] [PDF]
|
|
|
|
|
|
K. Ba-Charvet, Y von Boxberg, S Guazzi, E Boncinelli, and P Godement A potential role for the OTX2 homeoprotein in creating early 'highways' for axon extension in the rostral brain Development, January 11, 1998; 125(21): 4273 - 4282. [Abstract] [PDF]
|
|
|
|
|
|
M Hallonet, T Hollemann, R Wehr, N. Jenkins, N. Copeland, T Pieler, and P Gruss Vax1 is a novel homeobox-containing gene expressed in the developing anterior ventral forebrain Development, January 7, 1998; 125(14): 2599 - 2610. [Abstract] [PDF]
|
|
|
|
|
|
D Caric, D Gooday, R. Hill, S. McConnell, and D. Price Determination of the migratory capacity of embryonic cortical cells lacking the transcription factor Pax-6 Development, January 12, 1997; 124(24): 5087 - 5096. [Abstract] [PDF]
|
|
|
|
|
|
A Stoykova, M Gotz, P Gruss, and J Price Pax6-dependent regulation of adhesive patterning, R-cadherin expression and boundary formation in developing forebrain Development, January 10, 1997; 124(19): 3765 - 3777. [Abstract] [PDF]
|
|
|
|
|
|
Y. Shimoda, Y. Tajima, T. Osanai, A. Katsume, M. Kohara, T. Kudo, H. Narimatsu, N. Takashima, Y. Ishii, S. Nakamura, et al. Pax6 Controls the Expression of Lewis x Epitope in the Embryonic Forebrain by Regulating alpha 1,3-Fucosyltransferase IX Expression J. Biol. Chem., January 11, 2002; 277(3): 2033 - 2039. [Abstract] [Full Text] [PDF]
|
|
