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Development, Vol 124, Issue 12 2463-2476, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
H Lin and AC Spradling
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA. h.lin@cellbio.duke.edu
Germline stem cells play a pivotal role in gametogenesis; yet little is known about how they are formed, how they divide to self-renew, and how these processes are genetically controlled. Here we describe the self-renewing asymmetric division of germline stem cells in the Drosophila ovarian germline, as marked by the spectrosome, a cytoplasmic structure rich in membrane skeletal proteins. The ontogeny of the spectrosome marks the lineage of germline stem cells. We identified two new groups of mutations in which the divisional asymmetry is disrupted. The first, which we refer to as ovarette (ovt) mutations, was shown to correspond to a novel class of mutations in the pumilio locus. Since pumilio is known to posttranscriptionally repress the expression of target genes at earlier stages of germ cell development, our results suggest that a similar activity is needed to maintain germ line stem cells. We have also identified a second and novel gene, piwi, whose mutations abolish germline stem cell division.
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Z Liu, T Xie, and R Steward Lis1, the Drosophila homolog of a human lissencephaly disease gene, is required for germline cell division and oocyte differentiation Development, January 10, 1999; 126(20): 4477 - 4488. [Abstract] [PDF]
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F. King and H Lin Somatic signaling mediated by fs(1)Yb is essential for germline stem cell maintenance during Drosophila oogenesis Development, January 5, 1999; 126(9): 1833 - 1844. [Abstract] [PDF]
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M de Cuevas and A. Spradling Morphogenesis of the Drosophila fusome and its implications for oocyte specification Development, January 8, 1998; 125(15): 2781 - 2789. [Abstract] [PDF]
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S Styhler, A Nakamura, A Swan, B Suter, and P Lasko vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development Development, January 5, 1998; 125(9): 1569 - 1578. [Abstract] [PDF]
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A Forbes and R Lehmann Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells Development, January 2, 1998; 125(4): 679 - 690. [Abstract] [PDF]
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B Ohlstein and D McKearin Ectopic expression of the Drosophila Bam protein eliminates oogenic germline stem cells Development, January 9, 1997; 124(18): 3651 - 3662. [Abstract] [PDF]
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