|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 15 2875-2888, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
LA Wrischnik and CJ Kenyon
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0554, USA.
In C. elegans, six lateral epidermal stem cells, the seam cells V1-V6, are located in a row along the anterior-posterior (A/P) body axis. Anterior seam cells (V1-V4) undergo a fairly simple sequence of stem cell divisions and generate only epidermal cells. Posterior seam cells (V5 and V6) undergo a more complicated sequence of cell divisions that include additional rounds of stem cell proliferation and the production of neural as well as epidermal cells. In the wild type, activity of the gene lin-22 allows V1-V4 to generate their normal epidermal lineages rather than V5-like lineages. lin-22 activity is also required to prevent additional neurons from being produced by one branch of the V5 lineage. We find that the lin-22 gene exhibits homology to the Drosophila gene hairy, and that lin-22 activity represses neural development within the V5 lineage by blocking expression of the posterior-specific Hox gene mab-5 in specific cells. In addition, in order to prevent anterior V cells from generating V5-like lineages, wild-type lin-22 gene activity must inhibit (directly or indirectly) at least five downstream regulatory gene activities. In anterior body regions, lin-22(+) inhibits expression of the Hox gene mab-5. It also inhibits the activity of the achaete-scute homolog lin-32 and an unidentified gene that we postulate regulates stem cell division. Each of these three genes is required for the expression of a different piece of the ectopic V5-like lineages generated in lin-22 mutants. In addition, lin-22 activity prevents two other Hox genes, lin-39 and egl-5, from acquiring new activities within their normal domains of function along the A/P body axis. Some, but not all, of the patterning activities of lin-22 in C. elegans resemble those of hairy in Drosophila.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Karp and I. Greenwald Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans Genes & Dev., December 15, 2003; 17(24): 3100 - 3111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Van Auken, D. Weaver, B. Robertson, M. Sundaram, T. Saldi, L. Edgar, U. Elling, M. Lee, Q. Boese, and W. B. Wood Roles of the Homothorax/Meis/Prep homolog UNC-62 and the Exd/Pbx homologs CEH-20 and CEH-40 in C. elegans embryogenesis Development, March 13, 2003; 129(22): 5255 - 5268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang and S. W. Emmons Caenorhabditis elegans unc-37/groucho Interacts Genetically With Components of the Transcriptional Mediator Complex Genetics, February 1, 2002; 160(2): 799 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Nguyen, J. Round, R. O'Connell, P. Geurts, M. Funes-Duran, J. Wong, G. Jongeward, and C. A. Vierra Isolation and characterization of the activated B-cell factor 1 homolog in Caenorhabditis elegans Nucleic Acids Res., November 1, 2001; 29(21): 4423 - 4432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Kwon and J. Lee Biological significance of a universally conserved transcription mediator in metazoan developmental signaling pathways Development, August 15, 2001; 128(16): 3095 - 3104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koh and J. H. Rothman ELT-5 and ELT-6 are required continuously to regulate epidermal seam cell differentiation and cell fusion in C. elegans Development, August 1, 2001; 128(15): 2867 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Shioi, M. Shoji, M. Nakamura, T. Ishihara, I. Katsura, H. Fujisawa, and S. Takagi Mutations Affecting Nerve Attachment of Caenorhabditis elegans Genetics, April 1, 2001; 157(4): 1611 - 1622. [Abstract] [Full Text]
|
|
|
|
|
|
S Alper and C Kenyon REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity Development, January 5, 2001; 128(10): 1793 - 1804. [Abstract] [PDF]
|
|
|
|
|
|
H. Zhang and S. W. Emmons A C. elegans mediator protein confers regulatory selectivity on lineage-specific expression of a transcription factor gene Genes & Dev., September 1, 2000; 14(17): 2161 - 2172. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. E. Massari and C. Murre Helix-Loop-Helix Proteins: Regulators of Transcription in Eucaryotic Organisms Mol. Cell. Biol., January 15, 2000; 20(2): 429 - 440. [Full Text]
|
|
|
|
|
|
W Yi, J. Ross, and D Zarkower Mab-3 is a direct tra-1 target gene regulating diverse aspects of C. elegans male sexual development and behavior Development, January 10, 2000; 127(20): 4469 - 4480. [Abstract] [PDF]
|
|
|
|
|
|
H. Chamberlin and J. Thomas The bromodomain protein LIN-49 and trithorax-related protein LIN-59 affect development and gene expression in Caenorhabditis elegans Development, January 2, 2000; 127(4): 713 - 723. [Abstract] [PDF]
|
|
|
|
|
|
J. Maloof, J Whangbo, J. Harris, G. Jongeward, and C Kenyon A Wnt signaling pathway controls hox gene expression and neuroblast migration in C. elegans Development, January 1, 1999; 126(1): 37 - 49. [Abstract] [PDF]
|
|
|
|
|
|
N. Brown, S Kanekar, M. Vetter, P. Tucker, D. Gemza, and T Glaser Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis Development, January 12, 1998; 125(23): 4821 - 4833. [Abstract] [PDF]
|
|
|
|
 |
|
 C.J. Kenyon, J. Austin, M. Costa, D.W. Cowing, J.M. Harris, L. Honigberg, C.P. Hunter, J.N. Maloof, M.M. Muller-Immergluck, S.J. Salser, et al. The Dance of the Hox Genes: Patterning the Anteroposterior Body Axis of Caenorhabditis elegans Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 293 - 305. [Abstract] [PDF]
|
|
