Development, Vol 124, Issue 2 577-587, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration

T Schulte, KA Paschke, U Laessing, F Lottspeich and CA Stuermer
Faculty of Biology, University of Konstanz, Germany.

Fish--in contrast to mammals--regenerate retinal ganglion cell axons when the optic nerve is severed. Optic nerve injury leads to reexpression of proteins, which typically are first expressed in newly differentiated retinal ganglion cells and axons. Here we identified two new proteins of fish retinal ganglion cells, reggie-1 and reggie-2, with monoclonal antibody M802 and molecular cloning techniques. In normal fish, M802 stained the few retinal axons derived from newborn ganglion cells which in fish are added lifelong to the retinal margin. After optic nerve injury, however, M802 labeled all retinal ganglion cells and retinal axons throughout their path into tectum. Consistent with M802 staining, reggie-1 and reggie-2 mRNAs were present in lesioned retinal ganglion cells, as demonstrated by in situ hybridization, but were not detectable in their normal mature counterparts. In western blots with membrane proteins of the adult goldfish brain, M802 recognizes a 48x10(3) Mr protein band. At the amino acid level, 48x10(3) Mr reggie-1 and reggie-2 are 44% identical, lack transmembrane and membrane anchor domains, but appear membrane associated by ionic interactions. Reggie-1 and reggie-2 are homologous to 35x10(3) Mr ESA (human epidermal surface antigen) but are here identified as neuronal surface proteins, present on newly differentiated ganglion cells at the retinal margin and which are reexpressed in mature ganglion cells upon injury and during axonal regeneration.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				O. Alcazar, A. M. Hawkridge, T. S. Collier, S. W. Cousins, S. K. Bhattacharya, D. C. Muddiman, and M. E. Marin-Castano Proteomics Characterization of Cell Membrane Blebs in Human Retinal Pigment Epithelium Cells Mol. Cell. Proteomics, October 1, 2009; 8(10): 2201 - 2211. [Abstract] [Full Text] [PDF]

				C. Munderloh, G. P. Solis, V. Bodrikov, F. A. Jaeger, M. Wiechers, E. Malaga-Trillo, and C. A. O. Stuermer Reggies/Flotillins Regulate Retinal Axon Regeneration in the Zebrafish Optic Nerve and Differentiation of Hippocampal and N2a Neurons J. Neurosci., May 20, 2009; 29(20): 6607 - 6615. [Abstract] [Full Text] [PDF]

				C. Neumann-Giesen, I. Fernow, M. Amaddii, and R. Tikkanen Role of EGF-induced tyrosine phosphorylation of reggie-1/flotillin-2 in cell spreading and signaling to the actin cytoskeleton J. Cell Sci., February 1, 2007; 120(3): 395 - 406. [Abstract] [Full Text] [PDF]

				J. Liu, S. M. DeYoung, M. Zhang, L. H. Dold, and A. R. Saltiel The Stomatin/Prohibitin/Flotillin/HflK/C Domain of Flotillin-1 Contains Distinct Sequences That Direct Plasma Membrane Localization and Protein Interactions in 3T3-L1 Adipocytes J. Biol. Chem., April 22, 2005; 280(16): 16125 - 16134. [Abstract] [Full Text] [PDF]

				A. Santamaria, E. Castellanos, V. Gomez, P. Benedit, J. Renau-Piqueras, J. Morote, J. Reventos, T. M. Thomson, and R. Paciucci PTOV1 Enables the Nuclear Translocation and Mitogenic Activity of Flotillin-1, a Major Protein of Lipid Rafts Mol. Cell. Biol., March 1, 2005; 25(5): 1900 - 1911. [Abstract] [Full Text] [PDF]

				P. Hazarika, M. F. McCarty, V. G. Prieto, S. George, D. Babu, D. Koul, M. Bar-Eli, and M. Duvic Up-regulation of Flotillin-2 Is Associated with Melanoma Progression and Modulates Expression of the Thrombin Receptor Protease Activated Receptor 1 Cancer Res., October 15, 2004; 64(20): 7361 - 7369. [Abstract] [Full Text] [PDF]

				K. Haglund, I. Ivankovic-Dikic, N. Shimokawa, G. D. Kruh, and I. Dikic Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites J. Cell Sci., May 15, 2004; 117(12): 2557 - 2568. [Abstract] [Full Text] [PDF]

				L. Rajendran, M. Masilamani, S. Solomon, R. Tikkanen, C. A. O. Stuermer, H. Plattner, and H. Illges Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells PNAS, July 8, 2003; 100(14): 8241 - 8246. [Abstract] [Full Text] [PDF]

				I. C. Morrow, S. Rea, S. Martin, I. A. Prior, R. Prohaska, J. F. Hancock, D. E. James, and R. G. Parton Flotillin-1/Reggie-2 Traffics to Surface Raft Domains via a Novel Golgi-independent Pathway. IDENTIFICATION OF A NOVEL MEMBRANE TARGETING DOMAIN AND A ROLE FOR PALMITOYLATION J. Biol. Chem., December 6, 2002; 277(50): 48834 - 48841. [Abstract] [Full Text] [PDF]

				T. Nebl, K. N. Pestonjamasp, J. D. Leszyk, J. L. Crowley, S. W. Oh, and E. J. Luna Proteomic Analysis of a Detergent-resistant Membrane Skeleton from Neutrophil Plasma Membranes J. Biol. Chem., November 1, 2002; 277(45): 43399 - 43409. [Abstract] [Full Text] [PDF]

				M. Hekman, H. Hamm, A. V. Villar, B. Bader, J. Kuhlmann, J. Nickel, and U. R. Rapp Associations of B- and C-Raf with Cholesterol, Phosphatidylserine, and Lipid Second Messengers. PREFERENTIAL BINDING OF Raf TO ARTIFICIAL LIPID RAFTS J. Biol. Chem., June 28, 2002; 277(27): 24090 - 24102. [Abstract] [Full Text] [PDF]

				P. E. Bickel Lipid rafts and insulin signaling Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E1 - E10. [Abstract] [Full Text] [PDF]

				C. A.O. Stuermer, D. M. Lang, F. Kirsch, M. Wiechers, S.-O. Deininger, and H. Plattner Glycosylphosphatidyl Inositol-anchored Proteins and fyn Kinase Assemble in Noncaveolar Plasma Membrane Microdomains Defined by Reggie-1 and -2 Mol. Biol. Cell, October 1, 2001; 12(10): 3031 - 3045. [Abstract] [Full Text] [PDF]

				I. Gkantiragas, B. Brugger, E. Stuven, D. Kaloyanova, X.-Y. Li, K. Lohr, F. Lottspeich, F. T. Wieland, and J. B. Helms Sphingomyelin-enriched Microdomains at the Golgi Complex Mol. Biol. Cell, June 1, 2001; 12(6): 1819 - 1833. [Abstract] [Full Text] [PDF]

				U. Salzer and R. Prohaska Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts Blood, February 15, 2001; 97(4): 1141 - 1143. [Abstract] [Full Text] [PDF]

				B. Petrausch, R. Tabibiazar, T. Roser, Y. Jing, D. Goldman, C. A. O. Stuermer, N. Irwin, and L. I. Benowitz A Purine-Sensitive Pathway Regulates Multiple Genes Involved in Axon Regeneration in Goldfish Retinal Ganglion Cells J. Neurosci., November 1, 2000; 20(21): 8031 - 8041. [Abstract] [Full Text] [PDF]

				J. Trotter, C. Klein, and E.-M. Kramer GPI-Anchored Proteins and Glycosphingolipid-Rich Rafts: Platforms for Adhesion and Signaling Neuroscientist, August 1, 2000; 6(4): 271 - 284. [Abstract] [PDF]

				V. Michalova, B. W. Murray, H. Sultmann, and J. Klein A Contig Map of the Mhc Class I Genomic Region in the Zebrafish Reveals Ancient Synteny J. Immunol., May 15, 2000; 164(10): 5296 - 5305. [Abstract] [Full Text] [PDF]