|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 20 4163-4171, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
W Li, EM Skoulakis, RL Davis and N Perrimon
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
14-3-3 proteins have been shown to interact with Raf-1 and cause its activation when overexpressed. However, their precise role in Raf-1 activation is still enigmatic, as they are ubiquitously present in cells and found to associate with Raf-1 in vivo regardless of its activation state. We have analyzed the function of the Drosophila 14-3-3 gene leonardo (leo) in the Torso (Tor) receptor tyrosine kinase (RTK) pathway. In the syncytial blastoderm embryo, activation of Tor triggers the Ras/Raf/MEK pathway that controls the transcription of tailless (tll). We find that, in the absence of Tor, overexpression of leo is sufficient to activate tll expression. The effect of leo requires D-Raf and Ras1 activities but not KSR or DOS, two recently identified essential components of Drosophila RTK signaling pathways. Tor signaling is impaired in embryos derived from females lacking maternal expression of leo. We propose that binding to 14-3-3 by Raf is necessary but not sufficient for the activation of Raf and that overexpressed Drosophila 14-3-3 requires Ras1 to activate D-Raf.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. F. Acevedo, K. K. Tsigkari, S. Grammenoudi, and E. M. C. Skoulakis In Vivo Functional Specificity and Homeostasis of Drosophila 14-3-3 Proteins Genetics, September 1, 2007; 177(1): 239 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Huber, D. Vlasny, S. Jeckel, F. Stubenrauch, and T. Iftner Gene Profiling of Cottontail Rabbit Papillomavirus-Induced Carcinomas Identifies Upregulated Genes Directly Involved in Stroma Invasion as Shown by Small Interfering RNA-Mediated Gene Silencing J. Virol., July 15, 2004; 78(14): 7478 - 7489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li and W. X. Li Drosophila Gain-of-Function Mutant RTK Torso Triggers Ectopic Dpp and STAT Signaling Genetics, May 1, 2003; 164(1): 247 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. X. Li, H. Agaisse, B. Mathey-Prevot, and N. Perrimon Differential requirement for STAT by gain-of-function and wild-type receptor tyrosine kinase Torso in Drosophila Development, March 11, 2003; 129(18): 4241 - 4248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. Sehnke, J. M. DeLille, and R. J. Ferl Consummating Signal Transduction: The Role of 14-3-3 Proteins in the Completion of Signal-Induced Transitions in Protein Activity PLANT CELL, May 1, 2002; 14(90001): S339 - 354. [Full Text] [PDF]
|
|
|
|
|
|
V. Hsu, C. L. Zobel, E. J. Lambie, T. Schedl, and K. Kornfeld Caenorhabditis elegans lin-45 raf Is Essential for Larval Viability, Fertility and the Induction of Vulval Cell Fates Genetics, February 1, 2002; 160(2): 481 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Philip, S. F. Acevedo, and E. M. C. Skoulakis Conditional Rescue of Olfactory Learning and Memory Defects in Mutants of the 14-3-3zeta Gene leonardo J. Neurosci., November 1, 2001; 21(21): 8417 - 8425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Radke, K. Johnson, R. Guo, A. Davidson, and L. Ambrosio Drosophila-Raf Acts to Elaborate Dorsoventral Pattern in the Ectoderm of Developing Embryos Genetics, November 1, 2001; 159(3): 1031 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. J. Hamlet and L. A. Perkins Analysis of Corkscrew Signaling in the Drosophila Epidermal Growth Factor Receptor Pathway During Myogenesis Genetics, November 1, 2001; 159(3): 1073 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Kumar and K. Moses The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development Development, July 15, 2001; 128(14): 2689 - 2697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Su, D. H. Parry, B. Donahoe, C.-T. Chien, P. H. O'Farrell, and A. Purdy Cell cycle roles for two 14-3-3 proteins during Drosophila development J. Cell Sci., January 10, 2001; 114(19): 3445 - 3454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, E. Noll, and N. Perrimon Identification of Autosomal Regions Involved in Drosophila Raf Function Genetics, October 1, 2000; 156(2): 763 - 774. [Abstract] [Full Text]
|
|
|
|
|
|
W Li, M Melnick, and N Perrimon Dual function of Ras in Raf activation Development, January 12, 1998; 125(24): 4999 - 5008. [Abstract] [PDF]
|
|
