|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 21 4225-4234, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
TH Stratford, K Kostakopoulou and M Maden
Developmental Biology Research Centre, Division of Biological Sciences, Kings College London, UK.
The distribution of Hoxb-8 transcripts through the chick flank and early forelimb mirrors the distribution of polarizing activity in the flank at these early stages. Polarizing activity displayed by Hoxb-8-expressing tissue is only realised when placed adjacent to the AER and appears to be mediated through Shh induction, suggesting that Hoxb-8 may lie genetically upstream of Shh. Accordingly, Hoxb-8 expression is rapidly induced by retinoic acid (RA) treatment in the anterior of the forelimb in a spatial and temporal manner that is consistent with the induction of Shh and formation of the ZPA. Furthermore, inhibition of RA synthesis in the flank downregulates the expression of endogenous Hoxb-8 and results in the loss of Shh expression. However, once the ZPA has become established the posterior limb mesoderm displays resistance to the induction of Hoxb-8 expression. Grafting of ZPA cells to the anterior of a host limb renders the host anterior tissue resistant to RA-induced Hoxb-8 expression. These results indicate that Hoxb-8 expression may be regulated by the established ZPA through a negative feedback loop. The anterior AER also secretes an inhibitory factor, preventing RA-induced or already established Hoxb-8 expression in the cells immediately underneath the AER. Consistent with a role for Hoxb-8 in positioning of the forelimb ZPA, Hoxb-8 expression is not seen in RA-induced duplications at the anterior of the hindlimb. However, grafting of Hoxb-8-expressing tissue to the hindlimb can lead to Shh expression and similar duplications, suggesting that factors mediating ZPA formation are very similar in both wing and leg.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. A. Gillis, R. D. Dahn, and N. H. Shubin Shared developmental mechanisms pattern the vertebrate gill arch and paired fin skeletons PNAS, April 7, 2009; 106(14): 5720 - 5724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Galloway and C. J. Tabin Classic limb patterning models and the work of Dennis Summerbell Development, August 15, 2008; 135(16): 2683 - 2687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Capellini, G. Di Giacomo, V. Salsi, A. Brendolan, E. Ferretti, D. Srivastava, V. Zappavigna, and L. Selleri Pbx1/Pbx2 requirement for distal limb patterning is mediated by the hierarchical control of Hox gene spatial distribution and Shh expression Development, June 1, 2006; 133(11): 2263 - 2273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Ros, R. D. Dahn, M. Fernandez-Teran, K. Rashka, N. C. Caruccio, S. M. Hasso, J. J. Bitgood, J. J. Lancman, and J. F. Fallon The chick oligozeugodactyly (ozd) mutant lacks sonic hedgehog function in the limb Development, February 1, 2003; 130(3): 527 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Niederreither, J. Vermot, B. Schuhbaur, P. Chambon, and P. Dolle Embryonic retinoic acid synthesis is required for forelimb growth and anteroposterior patterning in the mouse Development, August 1, 2002; 129(15): 3563 - 3574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fernandez-Teran, M. E. Piedra, I. S. Kathiriya, D. Srivastava, J. C. Rodriguez-Rey, and M. A. Ros Role of dHAND in the anterior-posterior polarization of the limb bud: implications for the Sonic hedgehog pathway Development, May 15, 2000; 127(10): 2133 - 2142. [Abstract] [PDF]
|
|
|
|
|
|
A. Tavares, T Tsukui, and J. Izpisua Belmonte Evidence that members of the Cut/Cux/CDP family may be involved in AER positioning and polarizing activity during chick limb development Development, January 12, 2000; 127(23): 5133 - 5144. [Abstract] [PDF]
|
|
|
|
|
|
M Tanaka, M. Cohn, P Ashby, M Davey, P Martin, and C Tickle Distribution of polarizing activity and potential for limb formation in mouse and chick embryos and possible relationships to polydactyly Development, January 9, 2000; 127(18): 4011 - 4021. [Abstract] [PDF]
|
|
|
|
|
|
J Charite, D. McFadden, and E. Olson The bHLH transcription factor dHAND controls Sonic hedgehog expression and establishment of the zone of polarizing activity during limb development Development, January 6, 2000; 127(11): 2461 - 2470. [Abstract] [PDF]
|
|
|
|
|
|
A Zuniga and R Zeller Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity Development, January 1, 1999; 126(1): 13 - 21. [Abstract] [PDF]
|
|
|
|
 |
|
 G. R. Martin The roles of FGFs in the early development of vertebrate limbs Genes & Dev., June 1, 1998; 12(11): 1571 - 1586. [Full Text]
|
|
|
|
|
|
M Takahashi, K Tamura, D Buscher, H Masuya, S Yonei-Tamura, K Matsumoto, M Naitoh-Matsuo, J Takeuchi, K Ogura, T Shiroishi, et al. The role of Alx-4 in the establishment of anteroposterior polarity during vertebrate limb development Development, January 11, 1998; 125(22): 4417 - 4425. [Abstract] [PDF]
|
|
|
|
|
|
S Qu, S. Tucker, J. Ehrlich, J. Levorse, L. Flaherty, R Wisdom, and T. Vogt Mutations in mouse Aristaless-like4 cause Strong's luxoid polydactyly Development, January 7, 1998; 125(14): 2711 - 2721. [Abstract] [PDF]
|
|
