|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 1 51-60, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
H Ohuchi, J Takeuchi, H Yoshioka, Y Ishimaru, K Ogura, N Takahashi, T Ogura and S Noji
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan.
It has been reported that members of the fibroblast growth factor (FGF) family can induce additional limb formation in the flank of chick embryos. The phenotype of the ectopic limb depends on the somite level at which it forms: limbs in the anterior flank resemble wings, whereas those in the posterior flank resemble legs. Ectopic limbs located in the mid-flank appear chimeric, possessing characteristics of both wings and legs; feather buds are present in the anterior halves with scales and claws in the posterior halves. To study the mechanisms underlying the chimerism of these additional limbs, we cloned chick Tbx5 and Tbx4 to use as forelimb and hindlimb markers and examined their expression patterns in FGF-induced limb buds. We found that Tbx5 and Tbx4 were differentially expressed in the anterior and posterior halves of additional limb buds in the mid-flank, respectively, consistent with the chimeric patterns of the integument. A boundary of Tbx5/Tbx4 exists in all ectopic limbs, indicating that the additional limbs are essentially chimeric, although the degree of chimerism is dependent on the position. The boundary of Tbx5/Tbx4 expression is not fixed at a specific position within the interlimb region, but dependent upon where FGF was applied. Since the ectopic expression patterns of Tbx5/Tbx4 in the additional limbs are closely correlated with the patterns of their chimeric phenotypes, it is likely that Tbx5 and Tbx4 expression in the limb bud is involved in determination of the forelimb and hindlimb identities, respectively, in vertebrates.
This article has been cited by other articles:
|
|
 |
|
  N. Mercader, S. Fischer, and C. J. Neumann Prdm1 acts downstream of a sequential RA, Wnt and Fgf signaling cascade during zebrafish forelimb induction Development, August 1, 2006; 133(15): 2805 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lours and S. Dietrich The dissociation of the Fgf-feedback loop controls the limbless state of the neck Development, December 15, 2005; 132(24): 5553 - 5564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Logan Finger or toe: the molecular basis of limb identity Development, December 29, 2003; 130(26): 6401 - 6410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Naiche and V. E. Papaioannou Loss of Tbx4 blocks hindlimb development and affects vascularization and fusion of the allantois Development, June 15, 2003; 130(12): 2681 - 2693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Takeuchi, K. Koshiba-Takeuchi, T. Suzuki, M. Kamimura, K. Ogura, and T. Ogura Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade Development, June 15, 2003; 130(12): 2729 - 2739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rallis, B. G. Bruneau, J. Del Buono, C. E. Seidman, J. G. Seidman, S. Nissim, C. J. Tabin, and M. P. O. Logan Tbx5 is required for forelimb bud formation and continued outgrowth Development, June 15, 2003; 130(12): 2741 - 2751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Liu, E. Nakamura, V. Knezevic, S. Hunter, K. Thompson, and S. Mackem A role for the mesenchymal T-box gene Brachyury in AER formation during limb development Development, April 1, 2003; 130(7): 1327 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Ng, Y. Kawakami, D. Buscher, A. Raya, T. Itoh, C. M. Koth, C. R. Esteban, J. Rodriguez-Leon, D. M. Garrity, M. C. Fishman, et al. The limb identity gene Tbx5 promotes limb initiation by interacting with Wnt2b and Fgf10 Development, March 13, 2003; 129(22): 5161 - 5170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Agarwal, J. N. Wylie, J. Galceran, O. Arkhitko, C. Li, C. Deng, R. Grosschedl, and B. G. Bruneau Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo Development, February 1, 2003; 130(3): 623 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vitelli, I. Taddei, M. Morishima, E. N. Meyers, E. A. Lindsay, and A. Baldini A genetic link between Tbx1 and fibroblast growth factor signaling Development, January 10, 2002; 129(19): 4605 - 4611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Garrity, S. Childs, and M. C. Fishman The heartstrings mutation in zebrafish causes heart/fin Tbx5 deficiency syndrome Development, January 10, 2002; 129(19): 4635 - 4645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saito, S. Yonei-Tamura, K. Kano, H. Ide, and K. Tamura Specification and determination of limb identity: evidence for inhibitory regulation of Tbx gene expression Development, January 1, 2002; 129(1): 211 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L. Conlon, L. Fairclough, B. M. J. Price, E. S. Casey, and J. C. Smith Determinants of T box protein specificity Development, October 1, 2001; 128(19): 3749 - 3758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Ghosh, E. A. Packham, A. J. Bonser, T. E. Robinson, S. J. Cross, and J. D. Brook Characterization of the TBX5 binding site and analysis of mutations that cause Holt-Oram syndrome Hum. Mol. Genet., September 1, 2001; 10(18): 1983 - 1994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I Ruvinsky and J. Gibson-Brown Genetic and developmental bases of serial homology in vertebrate limb evolution Development, January 12, 2000; 127(24): 5233 - 5244. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Koshiba-Takeuchi, J. K. Takeuchi, K. Matsumoto, T. Momose, K. Uno, V. Hoepker, K. Ogura, N. Takahashi, H. Nakamura, K. Yasuda, et al. Tbx5 and the Retinotectum Projection Science, January 7, 2000; 287(5450): 134 - 137. [Abstract] [Full Text]
|
|
|
|
|
|
D Yelon, B Ticho, M. Halpern, I Ruvinsky, R. Ho, L. Silver, and D. Stainier The bHLH transcription factor hand2 plays parallel roles in zebrafish heart and pectoral fin development Development, January 6, 2000; 127(12): 2573 - 2582. [Abstract] [PDF]
|
|
|
|
 |
|
 M.-l. He, L. Wen, C. E. Campbell, J. Y. Wu, and Y. Rao Transcription repression by Xenopus ET and its human ortholog TBX3, a gene involved in ulnar-mammary syndrome PNAS, August 31, 1999; 96(18): 10212 - 10217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Szeto, C. Rodriguez-Esteban, A. K. Ryan, S. M. O'Connell, F. Liu, C. Kioussi, A. S. Gleiberman, J. C. Izpisúa-Belmonte, and M. G. Rosenfeld Role of the Bicoid-related homeodomain factor Pitx1 in specifying hindlimb morphogenesis and pituitary development Genes & Dev., February 15, 1999; 13(4): 484 - 494. [Abstract] [Full Text]
|
|
|
|
|
|
M. Horb and G. Thomsen Tbx5 is essential for heart development Development, January 4, 1999; 126(8): 1739 - 1751. [Abstract] [PDF]
|
|
|
|
|
|
G. R. Martin The roles of FGFs in the early development of vertebrate limbs Genes & Dev., June 1, 1998; 12(11): 1571 - 1586. [Full Text]
|
|
|
|
|
|
M Logan, H. Simon, and C Tabin Differential regulation of T-box and homeobox transcription factors suggests roles in controlling chick limb-type identity Development, January 8, 1998; 125(15): 2825 - 2835. [Abstract] [PDF]
|
|
|
|
|
|
J. Gibson-Brown, S. Agulnik, L. Silver, L Niswander, and V. Papaioannou Involvement of T-box genes Tbx2-Tbx5 in vertebrate limb specification and development Development, January 7, 1998; 125(13): 2499 - 2509. [Abstract] [PDF]
|
|
|
|
|
|
A Isaac, C Rodriguez-Esteban, A Ryan, M Altabef, T Tsukui, K Patel, C Tickle, and J. Izpisua-Belmonte Tbx genes and limb identity in chick embryo development Development, January 5, 1998; 125(10): 1867 - 1875. [Abstract] [PDF]
|
|
