|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 11 2053-2062, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
M Schubiger, AA Wade, GE Carney, JW Truman and M Bender
Department of Zoology, University of Washington, Seattle, WA 98195, USA.
During the metamorphic reorganization of the insect central nervous system, the steroid hormone 20-hydroxyecdysone induces a wide spectrum of cellular responses including neuronal proliferation, maturation, cell death and the remodeling of larval neurons into their adult forms. In Drosophila, expression of specific ecdysone receptor (EcR) isoforms has been correlated with particular responses, suggesting that different EcR isoforms may govern distinct steroid-induced responses in these cells. We have used imprecise excision of a P element to create EcR deletion mutants that remove the EcR-B promoter and therefore should lack EcR-B1 and EcR-B2 expression but retain EcR-A expression. Most of these EcR-B mutant animals show defects in larval molting, arresting at the boundaries between the three larval stages, while a smaller percentage of EcR-B mutants survive into the early stages of metamorphosis. Remodeling of larval neurons at metamorphosis begins with the pruning back of larval-specific dendrites and occurs as these cells are expressing high levels of EcR-B1 and little EcR-A. This pruning response is blocked in the EcR-B mutants despite the fact that adult-specific neurons, which normally express only EcR-A, can progress in their development. These observations support the hypothesis that different EcR isoforms control cell-type-specific responses during remodeling of the nervous system at metamorphosis.
This article has been cited by other articles:
|
|
 |
|
  T. Zhao, T. Gu, H. C. Rice, K. L. McAdams, K. M. Roark, K. Lawson, S. A. Gauthier, K. L. Reagan, and R. S. Hewes A Drosophila Gain-of-Function Screen for Candidate Genes Involved in Steroid-Dependent Neuroendocrine Cell Remodeling Genetics, February 1, 2008; 178(2): 883 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Allikian, G. Bhabha, P. Dospoy, A. Heydemann, P. Ryder, J. U. Earley, M. J. Wolf, H. A. Rockman, and E. M. McNally Reduced life span with heart and muscle dysfunction in Drosophila sarcoglycan mutants Hum. Mol. Genet., December 1, 2007; 16(23): 2933 - 2943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Browning, E. Martin, C. Loch, J.-M. Wurtz, D. Moras, R. H. Stote, A. P. Dejaegere, and I. M. L. Billas Critical Role of Desolvation in the Binding of 20-Hydroxyecdysone to the Ecdysone Receptor J. Biol. Chem., November 9, 2007; 282(45): 32924 - 32934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ninov, D. A. Chiarelli, and E. Martin-Blanco Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis Development, January 15, 2007; 134(2): 367 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Choi, G. Lee, and J. H. Park Programmed cell death mechanisms of identifiable peptidergic neurons in Drosophila melanogaster Development, June 1, 2006; 133(11): 2223 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, C.-H. J. Lee, S. Lin, and T. Lee Steroid hormone-dependent transformation of polyhomeotic mutant neurons in the Drosophila brain Development, April 1, 2006; 133(7): 1231 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. D. Brown, L. Cherbas, P. Cherbas, and J. W. Truman Use of time-lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila Development, January 15, 2006; 133(2): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schubiger, C. Carre, C. Antoniewski, and J. W. Truman Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing Development, December 1, 2005; 132(23): 5239 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Carre, D. Szymczak, J. Pidoux, and C. Antoniewski The Histone H3 Acetylase dGcn5 Is a Key Player in Drosophila melanogaster Metamorphosis Mol. Cell. Biol., September 15, 2005; 25(18): 8228 - 8238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Williams and J. W. Truman Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons Development, August 15, 2005; 132(16): 3631 - 3642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gates, G. Lam, J. A. Ortiz, R. Losson, and C. S. Thummel rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development Development, January 1, 2004; 131(1): 25 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Hu, L. Cherbas, and P. Cherbas Transcription Activation by the Ecdysone Receptor (EcR/USP): Identification of Activation Functions Mol. Endocrinol., April 1, 2003; 17(4): 716 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Peterson, G. E. Carney, B. J. Taylor, and K. White reaper is required for neuroblast apoptosis during Drosophila development Development, March 5, 2003; 129(6): 1467 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Park, V. Filippov, S. S. Gill, and M. E. Adams Deletion of the ecdysis-triggering hormone gene leads to lethal ecdysis deficiency Development, March 3, 2003; 129(2): 493 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cherbas, X. Hu, I. Zhimulev, E. Belyaeva, and P. Cherbas EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue Development, March 2, 2003; 130(2): 271 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Y. M. Rayburn, H. C. Gooding, S. P. Choksi, D. Maloney, A. R. Kidd III, D. E. Siekhaus, and M. Bender amontillado, the Drosophila Homolog of the Prohormone Processing Protease PC2, Is Required During Embryogenesis and Early Larval Development Genetics, January 1, 2003; 163(1): 227 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Zitnanova, M. E. Adams, and D. Zitnan Dual ecdysteroid action on the epitracheal glands and central nervous system preceding ecdysis of Manduca sexta J. Exp. Biol., March 12, 2002; 204(20): 3483 - 3495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kozlova and C. S. Thummel Spatial patterns of ecdysteroid receptor activation during the onset of Drosophila metamorphosis Development, January 4, 2002; 129(7): 1739 - 1750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Hewes, A. M. Schaefer, and P. H. Taghert The cryptocephal Gene (ATF4) Encodes Multiple Basic-Leucine Zipper Proteins Controlling Molting and Metamorphosis in Drosophila Genetics, August 1, 2000; 155(4): 1711 - 1723. [Abstract] [Full Text]
|
|
|
|
|
|
G. E. Carney and M. Bender The Drosophila ecdysone receptor (EcR) Gene Is Required Maternally for Normal Oogenesis Genetics, March 1, 2000; 154(3): 1203 - 1211. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Filonova, P. Bozhkov, V. Brukhin, G Daniel, B Zhivotovsky, and S von Arnold Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm, Norway spruce J. Cell Sci., January 12, 2000; 113(24): 4399 - 4411. [Abstract] [PDF]
|
|
|
|
|
|
M Yamada, T Murata, S Hirose, G Lavorgna, E Suzuki, and H Ueda Temporally restricted expression of transcription factor betaFTZ-F1: significance for embryogenesis, molting and metamorphosis in Drosophila melanogaster Development, January 12, 2000; 127(23): 5083 - 5092. [Abstract] [PDF]
|
|
|
|
|
|
V. Chavez, G Marques, J. Delbecque, K Kobayashi, M Hollingsworth, J Burr, J. Natzle, and M. O'Connor The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels Development, January 10, 2000; 127(19): 4115 - 4126. [Abstract] [PDF]
|
|
|
|
|
|
T Li and M Bender A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila Development, January 7, 2000; 127(13): 2897 - 2905. [Abstract] [PDF]
|
|
|
|
|
|
M Schubiger and J. Truman The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids Development, January 3, 2000; 127(6): 1151 - 1159. [Abstract] [PDF]
|
|
|
|
|
|
V. Brodu, B. Mugat, J.-Y. Roignant, J.-A. Lepesant, and C. Antoniewski Dual Requirement for the EcR/USP Nuclear Receptor and the dGATAb Factor in an Ecdysone Response in Drosophila melanogaster Mol. Cell. Biol., August 1, 1999; 19(8): 5732 - 5742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Freeman, A Dobritsa, P Gaines, W. Segraves, and J. Carlson The dare gene: steroid hormone production, olfactory behavior, and neural degeneration in Drosophila Development, January 10, 1999; 126(20): 4591 - 4602. [Abstract] [PDF]
|
|
|
|
|
|
T Lee, A Lee, and L Luo Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast Development, January 9, 1999; 126(18): 4065 - 4076. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Kraft, R. B. Levine, and L. L. Restifo The Steroid Hormone 20-Hydroxyecdysone Enhances Neurite Growth of Drosophila Mushroom Body Neurons Isolated during Metamorphosis J. Neurosci., November 1, 1998; 18(21): 8886 - 8899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hall and C. Thummel The RXR homolog ultraspiracle is an essential component of the Drosophila ecdysone receptor Development, January 12, 1998; 125(23): 4709 - 4717. [Abstract] [PDF]
|
|
