spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mu, X.
Right arrow Articles by Kimmel, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mu, X.
Right arrow Articles by Kimmel, A. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Development, Vol 125, Issue 18 3689-3698, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Sequence-specific protein interaction with a transcriptional enhancer involved in the autoregulated expression of cAMP receptor 1 in Dictyostelium

X Mu, B Lee, JM Louis and AR Kimmel
Laboratory of Cellular and Developmental Biology, NIDDK (Bldg 6/B1-22) and Howard Hughes Medical Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Major stages of Dictyostelium development are regulated by secreted, extracellular cAMP through activation of a serpentine receptor family. During early development, oscillations of extracellular cAMP mobilize cells for aggregation; later, continuous exposure to higher extracellular cAMP concentrations downregulates early gene expression and promotes cytodifferentiation and cell-specific gene expression. The cAMP receptor 1 gene CAR1 has two promoters that are differentially responsive to these extracellular cAMP stimuli. The early CAR1 promoter is induced by nM pulses of cAMP, which in turn are generated by CAR1-dependent activation of adenylyl cyclase (AC). Higher, non-fluctuating concentrations of cAMP will adapt this AC stimulus-response, repress the activated early promoter and induce the dormant late promoter. We now identify a critical element of the pulse-induced CAR1 promoter and a nuclear factor with sequence-specific interaction. Mutation of four nucleotides within the element prevents both in vitro protein binding and in vivo expression of an otherwise fully active early CAR1 promoter and multimerization of the wild-type, but not mutant, sequence will confer cAMP regulation to a quiescent heterologous promoter. These cis and trans elements, thus, constitute a part of the molecular response to the cAMP transmembrane signal cascade that regulates early development of Dictyostelium.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. Hirose, T. Mayanagi, C. Pears, A. Amagai, W. F. Loomis, and Y. Maeda
Transcriptional Switch of the dia1 and impA Promoter during the Growth/Differentiation Transition
Eukaryot. Cell, August 1, 2005; 4(8): 1477 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. A. Brzostowski, C. A. Parent, and A. R. Kimmel
A G{alpha}-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement
Genes & Dev., April 1, 2004; 18(7): 805 - 815.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1998