|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 19 3905-3914, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
O Cleaver and PA Krieg
Institute for Cellular and Molecular Biology and Department of Zoology, University of Texas at Austin, Austin, TX 78712, USA.
Angioblasts are precursor cells of the vascular endothelium which organize into the primitive blood vessels during embryogenesis. The molecular mechanisms underlying patterning of the embryonic vasculature remain unclear. Mutational analyses of the receptor tyrosine kinase flk-1 and its ligand vascular endothelial growth factor, VEGF, indicate that these molecules are critical for vascular development. Targeted ablation of the flk-1 gene results in complete failure of blood and vascular development (F. Shalaby et al. (1995) Nature 376, 62-66), while targeted ablation of the VEGF gene results in gross abnormalities in vascular patterning (P. Carmeliet et al. (1996) Nature 380, 435-439; N. Ferrara et al. (1996) Nature 380, 439-442). Here we report a role for VEGF in patterning the dorsal aorta of the Xenopus embryo. We show that the diffusible form of VEGF is expressed by the hypochord, which lies at the embryonic midline immediately dorsal to the location of the future dorsal aorta. We find that, initially, no flk-1-expressing angioblasts are present at this location, but that during subsequent development, angioblasts migrate from the lateral plate mesoderm to the midline where they form a single dorsal aorta. We have demonstrated that VEGF can act as a chemoattractant for angioblasts by ectopic expression of VEGF in the embryo. These results strongly suggest that localized sources of VEGF play a role in patterning the embryonic vasculature.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. F. Siekmann, C. Standley, K. E. Fogarty, S. A. Wolfe, and N. D. Lawson Chemokine signaling guides regional patterning of the first embryonic artery Genes & Dev., October 1, 2009; 23(19): 2272 - 2277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zheng, S. Vertuani, S. Nystrom, S. Audebert, I. Meijer, T. Tegnebratt, J.-P. Borg, P. Uhlen, A. Majumdar, and L. Holmgren Angiomotin-Like Protein 1 Controls Endothelial Polarity and Junction Stability During Sprouting Angiogenesis Circ. Res., July 31, 2009; 105(3): 260 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Kalin, N. E. Banziger-Tobler, M. Detmar, and A. W. Brandli An in vivo chemical library screen in Xenopus tadpoles reveals novel pathways involved in angiogenesis and lymphangiogenesis Blood, July 30, 2009; 114(5): 1110 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kazanskaya, B. Ohkawara, M. Heroult, W. Wu, N. Maltry, H. G. Augustin, and C. Niehrs The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development Development, November 15, 2008; 135(22): 3655 - 3664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Lee, T.-H. Chan, T.-C. Chen, B.-K. Liao, P.-P. Hwang, and H. Lee LPA1 is essential for lymphatic vessel development in zebrafish FASEB J, October 1, 2008; 22(10): 3706 - 3715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Hong, T. Kume, and R. T. Peterson Role of Crosstalk Between Phosphatidylinositol 3-Kinase and Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase Pathways in Artery-Vein Specification Circ. Res., September 12, 2008; 103(6): 573 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schmidt, K. Brixius, and W. Bloch Endothelial Precursor Cell Migration During Vasculogenesis Circ. Res., July 20, 2007; 101(2): 125 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tozer, M.-A. Bonnin, F. Relaix, S. Di Savino, P. Garcia-Villalba, P. Coumailleau, and D. Duprez Involvement of vessels and PDGFB in muscle splitting during chick limb development Development, July 15, 2007; 134(14): 2579 - 2591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Vieira, Q. Schwarz, and C. Ruhrberg Selective requirements for NRP1 ligands during neurovascular patterning Development, May 15, 2007; 134(10): 1833 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. L. Aranguren, A. Luttun, C. Clavel, C. Moreno, G. Abizanda, M. A. Barajas, B. Pelacho, M. Uriz, M. Arana, A. Echavarri, et al. In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells Blood, March 15, 2007; 109(6): 2634 - 2642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, F. Cerrato, H. Xu, F. Vitelli, M. Morishima, J. Vincentz, Y. Furuta, L. Ma, J. F. Martin, A. Baldini, et al. Tbx1 expression in pharyngeal epithelia is necessary for pharyngeal arch artery development Development, December 1, 2005; 132(23): 5307 - 5315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Jin, D. Beis, T. Mitchell, J.-N. Chen, and D. Y. R. Stainier Cellular and molecular analyses of vascular tube and lumen formation in zebrafish Development, December 1, 2005; 132(23): 5199 - 5209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sumanas, T. Jorniak, and S. Lin Identification of novel vascular endothelial-specific genes by the microarray analysis of the zebrafish cloche mutants Blood, July 15, 2005; 106(2): 534 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Murga, O. Fernandez-Capetillo, and G. Tosato Neuropilin-1 regulates attachment in human endothelial cells independently of vascular endothelial growth factor receptor-2 Blood, March 1, 2005; 105(5): 1992 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vokes, T. A. Yatskievych, R. L. Heimark, J. McMahon, A. P. McMahon, P. B. Antin, and P. A. Krieg Hedgehog signaling is essential for endothelial tube formation during vasculogenesis Development, September 1, 2004; 131(17): 4371 - 4380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Rupp, A. Czirok, and C. D. Little {alpha}v{beta}3 integrin-dependent endothelial cell dynamics in vivo Development, June 15, 2004; 131(12): 2887 - 2897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hogan, C. A. Ambler, D. L. Chapman, and V. L. Bautch The neural tube patterns vessels developmentally using the VEGF signaling pathway Development, April 1, 2004; 131(7): 1503 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Germani, A. Di Carlo, A. Mangoni, S. Straino, C. Giacinti, P. Turrini, P. Biglioli, and M. C. Capogrossi Vascular Endothelial Growth Factor Modulates Skeletal Myoblast Function Am. J. Pathol., October 1, 2003; 163(4): 1417 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shoji, S. Isogai, M. Sato-Maeda, M. Obinata, and J. Y. Kuwada Semaphorin3a1 regulates angioblast migration and vascular development in zebrafish embryos Development, July 15, 2003; 130(14): 3227 - 3236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Walmsley, A. Ciau-Uitz, and R. Patient Adult and embryonic blood and endothelium derive from distinct precursor populations which are differentially programmed by BMP in Xenopus Development, March 14, 2003; 129(24): 5683 - 5695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Damert, L. Miquerol, M. Gertsenstein, W. Risau, and A. Nagy Insufficient VEGFA activity in yolk sac endoderm compromises haematopoietic and endothelial differentiation Development, March 6, 2003; 129(8): 1881 - 1892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Childs, J.-N. Chen, D. M. Garrity, and M. C. Fishman Patterning of angiogenesis in the zebrafish embryo Development, March 4, 2003; 129(4): 973 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ruhrberg, H. Gerhardt, M. Golding, R. Watson, S. Ioannidou, H. Fujisawa, C. Betsholtz, and D. T. Shima Spatially restricted patterning cues provided by heparin-binding VEGF-A control blood vessel branching morphogenesis Genes & Dev., October 15, 2002; 16(20): 2684 - 2698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. McLeod, M. Taomoto, J. Cao, Z. Zhu, L. Witte, and G. A. Lutty Localization of VEGF Receptor-2 (KDR/Flk-1) and Effects of Blocking It in Oxygen-Induced Retinopathy Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 474 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Latimer, X. Dong, Y. Markov, and B. Appel Delta-Notch signaling induces hypochord development in zebrafish Development, January 6, 2002; 129(11): 2555 - 2563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Vokes and P. A. Krieg Endoderm is required for vascular endothelial tube formation, but not for angioblast specification Development, January 2, 2002; 129(3): 775 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Lawson, N. Scheer, V. N. Pham, C.-H. Kim, A. B. Chitnis, J. A. Campos-Ortega, and B. M. Weinstein Notch signaling is required for arterial-venous differentiation during embryonic vascular development Development, October 1, 2001; 128(19): 3675 - 3683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Amann and E. Ritz Microvascular disease--the Cinderella of uraemic heart disease Nephrol. Dial. Transplant., October 1, 2000; 15(10): 1493 - 1503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Haigh, H. Gerber, N Ferrara, and E. Wagner Conditional inactivation of VEGF-A in areas of collagen2a1 expression results in embryonic lethality in the heterozygous state Development, January 4, 2000; 127(7): 1445 - 1453. [Abstract] [PDF]
|
|
|
|
|
|
O Cleaver, D. Seufert, and P. Krieg Endoderm patterning by the notochord: development of the hypochord in Xenopus Development, January 2, 2000; 127(4): 869 - 879. [Abstract] [PDF]
|
|
|
|
|
|
P. Helbling, D. Saulnier, and A. Brandli The receptor tyrosine kinase EphB4 and ephrin-B ligands restrict angiogenic growth of embryonic veins in Xenopus laevis Development, January 1, 2000; 127(2): 269 - 278. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Hidaka, W. L. Stanford, and A. Bernstein Conditional requirement for the Flk-1 receptor in the in vitro generation of early hematopoietic cells PNAS, June 22, 1999; 96(13): 7370 - 7375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Schuh, P. Faloon, Q.-L. Hu, M. Bhimani, and K. Choi In vitro hematopoietic and endothelial potential of flk-1-/- embryonic stem cells and embryos PNAS, March 2, 1999; 96(5): 2159 - 2164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lammert, O. Cleaver, and D. Melton Induction of Pancreatic Differentiation by Signals from Blood Vessels Science, October 19, 2001; 294(5542): 564 - 567. [Abstract] [Full Text] [PDF]
|
|
