|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 23 4627-4635, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
KT Jones, C Soeller and MB Cannell
Department of Anatomy and Developmental Biology, University College, London WC1E 6BT, UK.
Mouse sperm-egg fusion was examined using two-photon and confocal microscopy. A delay of several minutes occurred between the first observable event of fusion (which was the diffusion of Ca2+-sensitive dyes from egg into sperm) and any change in egg cytoplasmic Ca2+. When indo-1 dextran was used to obtain ratiometric two-photon images, there was no detectable local increase in egg cytoplasmic Ca2+ near the site of sperm fusion. However, the sperm underwent a Ca2+ transient which appeared to be coincident with the egg cytoplasm Ca2+ transient, which suggested that there was a high permeability pathway for Ca2+ between egg and sperm. To exclude this pathway from providing trigger Ca2+ for the egg transient, we reduced bathing [Ca2+] to approx. 18 microM and 13nM (with EGTA). In these conditions the first egg Ca2+ transient was not prevented, which makes an obligatory role for extracellular Ca2+ in the initiation of the egg Ca2+ transient unlikely. Both FITC-albumin (70 kDa) and 10 kDa dextran-linked Ca2+ indicators were able to diffuse into the sperm from the egg. In addition, phycoerythrin (240 kDa) rapidly diffused into the sperm shortly after fusion (but before any changes in Ca2+ occurred). This suggests that the 'pore(s)' that form during sperm-egg fusion must be at least 8 nm in diameter. These data are compatible with the idea that a diffusible sperm protein could trigger the observed changes in intracellular Ca2+ in the egg, but do not exclude the possibility that other second messengers are generated during sperm-egg fusion.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S.-Y. Yoon and R. A Fissore Release of phospholipase C {zeta}and [Ca2+]i oscillation-inducing activity during mammalian fertilization Reproduction, November 1, 2007; 134(5): 695 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Whitaker Calcium at Fertilization and in Early Development Physiol Rev, January 1, 2006; 86(1): 25 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T Jones Mammalian egg activation: from Ca2+ spiking to cell cycle progression Reproduction, December 1, 2005; 130(6): 813 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Venables, C. Dalgliesh, M. P. Paronetto, L. Skitt, J. K. Thornton, P. T. Saunders, C. Sette, K. T. Jones, and D. J. Elliott SIAH1 targets the alternative splicing factor T-STAR for degradation by the proteasome Hum. Mol. Genet., July 15, 2004; 13(14): 1525 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Bedford, M. Kurokawa, K. Hinrichs, and R. A. Fissore Patterns of Intracellular Calcium Oscillations in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible Explanations for the Low Success of This Assisted Reproduction Technique in the Horse Biol Reprod, April 1, 2004; 70(4): 936 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Jones Turning it on and off: M-phase promoting factor during meiotic maturation and fertilization Mol. Hum. Reprod., January 1, 2004; 10(1): 1 - 5. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Saunders, M. G. Larman, J. Parrington, L. J. Cox, J. Royse, L. M. Blayney, K. Swann, and F. A. Lai PLC{zeta}: a sperm-specific trigger of Ca2+ oscillations in eggs and embryo development Development, August 1, 2002; 129(15): 3533 - 3544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Halet, R. Tunwell, T. Balla, K. Swann, and J. Carroll The dynamics of plasma membrane PtdIns(4,5)P2 at fertilization of mouse eggs J. Cell Sci., May 15, 2002; 115(10): 2139 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Campbell, W. A. Reed, and K. L. White Ability of Integrins to Mediate Fertilization, Intracellular Calcium Release, and Parthenogenetic Development in Bovine Oocytes Biol Reprod, June 1, 2000; 62(6): 1702 - 1709. [Abstract] [Full Text]
|
|
|
|
|
|
A McDougall, M Levasseur, A. O'Sullivan, and K. Jones Cell cycle-dependent repetitive Ca(2+ )waves induced by a cytosolic sperm extract in mature ascidian eggs mimic those observed at fertilization J. Cell Sci., January 10, 2000; 113(19): 3453 - 3462. [Abstract] [PDF]
|
|
|
|
|
|
L. Runft and L. Jaffe Sperm extract injection into ascidian eggs signals Ca(2+) release by the same pathway as fertilization Development, January 8, 2000; 127(15): 3227 - 3236. [Abstract] [PDF]
|
|
|
|
|
|
W. Hable and D. Kropf Sperm entry induces polarity in fucoid zygotes Development, January 2, 2000; 127(3): 493 - 501. [Abstract] [PDF]
|
|
