Development, Vol 125, Issue 24 5069-5078, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

decapentaplegic is required for arrest in G1 phase during Drosophila eye development

J Horsfield, A Penton, J Secombe, FM Hoffman and H Richardson
Department of Genetics, Adelaide University, South Australia, Australia.

During eye development in Drosophila, cell cycle progression is coordinated with differentiation. Prior to differentiation, cells arrest in G1 phase anterior to and within the morphogenetic furrow. We show that Decapentaplegic (Dpp), a TGF-&bgr; family member, is required to establish this G1 arrest, since Dpp-unresponsive cells located in the anterior half of the morphogenetic furrow show ectopic S phases and ectopic expression of the cell cycle regulators Cyclins A, E and B. Conversely, ubiquitous over-expression of Dpp in the eye imaginal disc transiently inhibits S phase without affecting Cyclin E or Cyclin A abundance. This Dpp-mediated inhibition of S phase occurs independently of the Cyclin A inhibitor Roughex and of the expression of Dacapo, a Cyclin E-Cdk2 inhibitor. Furthermore, Dpp-signaling genes interact genetically with a hypomorphic cyclin E allele. Taken together our results suggest that Dpp acts to induce G1 arrest in the anterior part of the morphogenetic furrow by a novel inhibitory mechanism. In addition, our results provide evidence for a Dpp-independent mechanism that acts in the posterior part of the morphogenetic furrow to maintain G1 arrest.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. C. Zhu, J. Q. Boone, P. A. Jensen, S. Hanna, L. Podemski, J. Locke, C. Q. Doe, and M. B. O'Connor Drosophila Activin- and the Activin-like product Dawdle function redundantly to regulate proliferation in the larval brain Development, February 1, 2008; 135(3): 513 - 521. [Abstract] [Full Text] [PDF]

				A. M. Ambrus, B. N. Nicolay, V. I. Rasheva, R. J. Suckling, and M. V. Frolov dE2F2-Independent Rescue of Proliferation in Cells Lacking an Activator dE2F1 Mol. Cell. Biol., December 15, 2007; 27(24): 8561 - 8570. [Abstract] [Full Text] [PDF]

				C. Jones, R. Reifegerste, and K. Moses Characterization of Drosophila mini-me, a Gene Required for Cell Proliferation and Survival Genetics, June 1, 2006; 173(2): 793 - 808. [Abstract] [Full Text] [PDF]

				A. Brumby, J. Secombe, J. Horsfield, M. Coombe, N. Amin, D. Coates, R. Saint, and H. Richardson A Genetic Screen for Dominant Modifiers of a cyclin E Hypomorphic Mutation Identifies Novel Regulators of S-Phase Entry in Drosophila Genetics, September 1, 2004; 168(1): 227 - 251. [Abstract] [Full Text] [PDF]

				C. Thomas and P. W. Ingham Hedgehog Signaling in the Drosophila Eye and Head: An Analysis of the Effects of Different patched Trans-heterozygotes Genetics, December 1, 2003; 165(4): 1915 - 1928. [Abstract] [Full Text] [PDF]

				S. Szuplewski, B. Kottler, and R. Terracol The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth Development, August 15, 2003; 130(16): 3651 - 3662. [Abstract] [Full Text] [PDF]

				J. Bessa, B. Gebelein, F. Pichaud, F. Casares, and R. S. Mann Combinatorial control of Drosophila eye development by Eyeless, Homothorax, and Teashirt Genes & Dev., September 15, 2002; 16(18): 2415 - 2427. [Abstract] [Full Text] [PDF]

				A.-S. K. Tseng and I. K. Hariharan An Overexpression Screen in Drosophila for Genes That Restrict Growth or Cell-Cycle Progression in the Developing Eye Genetics, September 1, 2002; 162(1): 229 - 243. [Abstract] [Full Text] [PDF]

				H. K. Shamloula, M. P. Mbogho, A. C. Pimentel, Z. M. A. Chrzanowska-Lightowlers, V. Hyatt, H. Okano, and T. R. Venkatesh rugose (rg), a Drosophila A kinase Anchor Protein, Is Required for Retinal Pattern Formation and Interacts Genetically With Multiple Signaling Pathways Genetics, June 1, 2002; 161(2): 693 - 710. [Abstract] [Full Text] [PDF]

				F. Chanut, K. Woo, S. Pereira, T. J. Donohoe, S.-Y. Chang, T. R. Laverty, A. P. Jarman, and U. Heberlein Rough eye Is a Gain-of-Function Allele of amos That Disrupts Regulation of the Proneural Gene atonal During Drosophila Retinal Differentiation Genetics, February 1, 2002; 160(2): 623 - 635. [Abstract] [Full Text] [PDF]

				S. Roy and P. W. Ingham Hedgehogs tryst with the cell cycle J. Cell Sci., January 12, 2002; 115(23): 4393 - 4397. [Abstract] [Full Text] [PDF]

				I. Kostic and R. Roy Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegans Development, January 5, 2002; 129(9): 2155 - 2165. [Abstract] [Full Text] [PDF]

				J. P. Kumar and K. Moses The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development Development, July 15, 2001; 128(14): 2689 - 2697. [Abstract] [Full Text] [PDF]

				C. Brennan, T. Li, M Bender, F Hsiung, and K Moses Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye Development, January 1, 2001; 128(1): 1 - 11. [Abstract] [PDF]

				L Jones, H Richardson, and R Saint Tissue-specific regulation of cyclin E transcription during Drosophila melanogaster embryogenesis Development, January 11, 2000; 127(21): 4619 - 4630. [Abstract] [PDF]

				J Curtiss and M Mlodzik Morphogenetic furrow initiation and progression during eye development in Drosophila: the roles of decapentaplegic, hedgehog and eyes absent Development, January 3, 2000; 127(6): 1325 - 1336. [Abstract] [PDF]

				W Du Suppression of the rbf null mutants by a de2f1 allele that lacks transactivation domain Development, January 1, 2000; 127(2): 367 - 379. [Abstract] [PDF]

				O Zilian, E Frei, R Burke, D Brentrup, T Gutjahr, P. Bryant, and M Noll double-time is identical to discs overgrown, which is required for cell survival, proliferation and growth arrest in Drosophila imaginal discs Development, January 12, 1999; 126(23): 5409 - 5420. [Abstract] [PDF]