|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 3 545-556, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
J van der Wees, JG Schilthuis, CH Koster, H Diesveld-Schipper, GE Folkers, PT van der Saag, MI Dawson, K Shudo, B van der Burg and AJ Durston
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Retinoids regulate gene expression via nuclear retinoic acid receptors, the RARs and RXRs. To investigate the functions of retinoid receptors during early neural development, we expressed a dominant negative RARbeta in early Xenopus embryos. We obtained evidence that dominant negative RARbeta specifically inhibits RAR/RXR heterodimer-mediated, but not RXR homodimer-mediated, transactivation. Both all-trans- and 9-cis-RA-induced teratogenesis were, however, efficiently opposed by ectopic expression of dominant negative RARbeta, indicating that only RAR/RXR transactivation is required for retinoid teratogenesis by each of these ligands. Experiments with two RXR-selective ligands confirmed that activation of RXR homodimers does not cause retinoid teratogenesis. Dominant negative RARbeta thus specifically interferes with the retinoid signalling pathway that is responsible for retinoid teratogenesis. Dominant negative RARbeta-expressing embryos had a specific developmental phenotype leading to disorganization of the hindbrain. Mauthner cell multiplications in the posterior hindbrain, and (both anteriorly and posteriorly) expanded Krox-20 expression domains indicated (partial) transformation of a large part of the hindbrain into (at least partial) rhombomere 3, 4 and/or 5 identity. In contrast, the fore- and midbrain and spinal cord appeared to be less affected. These data indicate that RARs play a role in patterning the hindbrain.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Strate, T. H. Min, D. Iliev, and E. M. Pera Retinol dehydrogenase 10 is a feedback regulator of retinoic acid signalling during axis formation and patterning of the central nervous system Development, February 1, 2009; 136(3): 461 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. O. Sirbu, L. Gresh, J. Barra, and G. Duester Shifting boundaries of retinoic acid activity control hindbrain segmental gene expression Development, June 1, 2005; 132(11): 2611 - 2622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shiotsugu, Y. Katsuyama, K. Arima, A. Baxter, T. Koide, J. Song, R. A. S. Chandraratna, and B. Blumberg Multiple points of interaction between retinoic acid and FGF signaling during embryonic axis formation Development, June 1, 2004; 131(11): 2653 - 2667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Halilagic, M. H. Zile, and M. Studer A novel role for retinoids in patterning the avian forebrain during presomite stages Development, May 15, 2003; 130(10): 2039 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Matt, N. B. Ghyselinck, O. Wendling, P. Chambon, and M. Mark Retinoic acid-induced developmental defects are mediated by RAR{beta}/RXR heterodimers in the pharyngeal endoderm Development, May 15, 2003; 130(10): 2083 - 2093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mirshahi, E. Ayani, C. Nicolas, N. Golestaneh, P. Ferrari, F. Valamanesh, and M. K. Agarwal The Blockade of Mineralocorticoid Hormone Signaling Provokes Dramatic Teratogenesis in Cultured Rat Embryos International Journal of Toxicology, May 1, 2002; 21(3): 191 - 199. [Abstract] [PDF]
|
|
|
|
|
|
G. Begemann, T. F. Schilling, G.-J. Rauch, R. Geisler, and P. W. Ingham The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain Development, August 15, 2001; 128(16): 3081 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Koide, M. Downes, R. A.S. Chandraratna, B. Blumberg, and K. Umesono Active repression of RAR signaling is required for head formation Genes & Dev., August 15, 2001; 15(16): 2111 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dupe and A. Lumsden Hindbrain patterning involves graded responses to retinoic acid signalling Development, June 15, 2001; 128(12): 2199 - 2208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Wendling, N. B. Ghyselinck, P. Chambon, and M. Mark Roles of retinoic acid receptors in early embryonic morphogenesis and hindbrain patterning Development, June 1, 2001; 128(11): 2031 - 2038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.L. Young, R.A. Schneider, D. Hu, and J.A. Helms Genetic and Teratogenic Approaches To Craniofacial Development Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(3): 304 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Niederreither, J Vermot, B Schuhbaur, P Chambon, and P Dolle Retinoic acid synthesis and hindbrain patterning in the mouse embryo Development, January 1, 2000; 127(1): 75 - 85. [Abstract] [PDF]
|
|
|
|
|
|
V Dupe, N. Ghyselinck, O Wendling, P Chambon, and M Mark Key roles of retinoic acid receptors alpha and beta in the patterning of the caudal hindbrain, pharyngeal arches and otocyst in the mouse Development, January 11, 1999; 126(22): 5051 - 5059. [Abstract] [PDF]
|
|
