Development, Vol 125, Issue 6 1049-1057, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Systematic gain-of-function genetics in Drosophila

P Rorth, K Szabo, A Bailey, T Laverty, J Rehm, GM Rubin, K Weigmann, M Milan, V Benes, W Ansorge and SM Cohen
Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA. rorth@mail1.ciwemb.edu

A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes. Among many novel genes, the screen identified known genes, including hedgehog and decapentaplegic, implicated in these processes. A genetic interaction screen for suppressors of a cell migration defect in a hypomorphic slow border cells mutant identified known genes with likely roles in tyrosine kinase signaling and control of actin cytoskeleton, among many novel genes. These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. V. Myrick, F. Huet, S. E. Mohr, I. Alvarez-Garcia, J. T. Lu, M. A. Smith, M. A. Crosby, and W. M. Gelbart Large-Scale Functional Annotation and Expanded Implementations of the P{wHy} Hybrid Transposon in the Drosophila melanogaster Genome Genetics, July 1, 2009; 182(3): 653 - 660. [Abstract] [Full Text] [PDF]

				K. S. Weiler and S. Chatterjee The Multi-AT-Hook Chromosomal Protein of Drosophila melanogaster, D1, Is Dispensable for Viability Genetics, May 1, 2009; 182(1): 145 - 159. [Abstract] [Full Text] [PDF]

				M. A. Ayliffe, A. Agostino, B. C. Clarke, R. Furbank, S. von Caemmerer, and A. J. Pryor Suppression of the Barley uroporphyrinogen III synthase Gene by a Ds Activation Tagging Element Generates Developmental Photosensitivity PLANT CELL, March 1, 2009; 21(3): 814 - 831. [Abstract] [Full Text] [PDF]

				V. Maybeck and K. Roper A Targeted Gain-of-Function Screen Identifies Genes Affecting Salivary Gland Morphogenesis/Tubulogenesis in Drosophila Genetics, February 1, 2009; 181(2): 543 - 565. [Abstract] [Full Text] [PDF]

				D. Chen, J. Berger, M. Fellner, and T. Suzuki FLYSNPdb: a high-density SNP database of Drosophila melanogaster Nucleic Acids Res., January 1, 2009; 37(suppl_1): D567 - D570. [Abstract] [Full Text] [PDF]

				L.-E. Jao, L. Maddison, W. Chen, and S. M. Burgess Using retroviruses as a mutagenesis tool to explore the zebrafish genome Brief Funct Genomic Proteomic, November 1, 2008; 7(6): 427 - 443. [Abstract] [Full Text] [PDF]

				N. Vereshchagina, M.-C. Ramel, E. Bitoun, and C. Wilson The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila J. Cell Sci., October 15, 2008; 121(20): 3383 - 3392. [Abstract] [Full Text] [PDF]

				S. B. Uthaman, T. A. Godenschwege, and R. K. Murphey A Mechanism Distinct from Highwire for the Drosophila Ubiquitin Conjugase Bendless in Synaptic Growth and Maturation J. Neurosci., August 20, 2008; 28(34): 8615 - 8623. [Abstract] [Full Text] [PDF]

				T. D. Southall, D. A. Elliott, and A. H. Brand The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila CSH Protocols, July 1, 2008; 2008(8): pdb.top49 - pdb.top49. [Abstract] [Full Text]

				F. Friggi-Grelin, L. Lavenant-Staccini, and P. Therond Control of Antagonistic Components of the Hedgehog Signaling Pathway by microRNAs in Drosophila Genetics, May 1, 2008; 179(1): 429 - 439. [Abstract] [Full Text] [PDF]

				A. Khokhar, N. Chen, J.-P. Yuan, Y. Li, G. N. Landis, G. Beaulieu, H. Kaur, and J. Tower Conditional Switches for Extracellular Matrix Patterning in Drosophila melanogaster Genetics, March 1, 2008; 178(3): 1283 - 1293. [Abstract] [Full Text] [PDF]

				W. Cao, H.-J. Song, T. Gangi, A. Kelkar, I. Antani, D. Garza, and M. Konsolaki Identification of Novel Genes That Modify Phenotypes Induced by Alzheimer's {beta}-Amyloid Overexpression in Drosophila Genetics, March 1, 2008; 178(3): 1457 - 1471. [Abstract] [Full Text] [PDF]

				T. Zhao, T. Gu, H. C. Rice, K. L. McAdams, K. M. Roark, K. Lawson, S. A. Gauthier, K. L. Reagan, and R. S. Hewes A Drosophila Gain-of-Function Screen for Candidate Genes Involved in Steroid-Dependent Neuroendocrine Cell Remodeling Genetics, February 1, 2008; 178(2): 883 - 901. [Abstract] [Full Text] [PDF]

				F. Bejarano, C. M. Luque, H. Herranz, G. Sorrosal, N. Rafel, T. T. Pham, and M. Milan A Gain-of-Function Suppressor Screen for Genes Involved in Dorsal-Ventral Boundary Formation in the Drosophila Wing Genetics, January 1, 2008; 178(1): 307 - 323. [Abstract] [Full Text] [PDF]

				C. M. Miller, A. Page-McCaw, and H. T. Broihier Matrix metalloproteinases promote motor axon fasciculation in the Drosophila embryo Development, January 1, 2008; 135(1): 95 - 109. [Abstract] [Full Text] [PDF]

				R. Bastock and D. Strutt The planar polarity pathway promotes coordinated cell migration during Drosophila oogenesis Development, September 1, 2007; 134(17): 3055 - 3064. [Abstract] [Full Text] [PDF]

				M. Furriols, G. Ventura, and J. Casanova Two distinct but convergent groups of cells trigger Torso receptor tyrosine kinase activation by independently expressing torso-like PNAS, July 10, 2007; 104(28): 11660 - 11665. [Abstract] [Full Text] [PDF]

				J. Mathieu, H.-H. Sung, C. Pugieux, J. Soetaert, and P. Rorth A Sensitized PiggyBac-Based Screen for Regulators of Border Cell Migration in Drosophila Genetics, July 1, 2007; 176(3): 1579 - 1590. [Abstract] [Full Text] [PDF]

				J. T. Felix, M. Magarinos, and F. J. Diaz-Benjumea Nab controls the activity of the zinc-finger transcription factors Squeeze and Rotund in Drosophila development Development, May 15, 2007; 134(10): 1845 - 1852. [Abstract] [Full Text] [PDF]

				H. Huang and G. G. Haddad Drosophila dMRP4 regulates responsiveness to O2 deprivation and development under hypoxia Physiol Genomics, May 11, 2007; 29(3): 260 - 266. [Abstract] [Full Text] [PDF]

				N. C. Grieder, I. Charlafti, U. Kloter, H. Jackle, U. Schafer, and W. J. Gehring Misexpression Screen in Drosophila melanogaster Aiming to Reveal Novel Factors Involved in Formation of Body Parts Genetics, April 1, 2007; 175(4): 1707 - 1718. [Abstract] [Full Text] [PDF]

				C. Molnar, A. Lopez-Varea, R. Hernandez, and J. F. de Celis A Gain-of-Function Screen Identifying Genes Required for Vein Formation in the Drosophila melanogaster Wing Genetics, November 1, 2006; 174(3): 1635 - 1659. [Abstract] [Full Text] [PDF]

				N. M. Viquez, C. R. Li, Y. P. Wairkar, and A. DiAntonio The B' Protein Phosphatase 2A Regulatory Subunit well-rounded Regulates Synaptic Growth and Cytoskeletal Stability at the Drosophila Neuromuscular Junction J. Neurosci., September 6, 2006; 26(36): 9293 - 9303. [Abstract] [Full Text] [PDF]

				R. S. Hawley and W. D. Gilliland Sometimes the Result Is Not the Answer: The Truths and the Lies That Come From Using the Complementation Test Genetics, September 1, 2006; 174(1): 5 - 15. [Abstract] [Full Text] [PDF]

				T. E. Rusten, L. M.W. Rodahl, K. Pattni, C. Englund, C. Samakovlis, S. Dove, A. Brech, and H. Stenmark Fab1 Phosphatidylinositol 3-Phosphate 5-Kinase Controls Trafficking but Not Silencing of Endocytosed Receptors Mol. Biol. Cell, September 1, 2006; 17(9): 3989 - 4001. [Abstract] [Full Text] [PDF]

				H. Wen, T. M. Weiger, T. S. Ferguson, M. Shahidullah, S. S. Scott, and I. B. Levitan A Drosophila KCNQ Channel Essential for Early Embryonic Development J. Neurosci., November 2, 2005; 25(44): 10147 - 10156. [Abstract] [Full Text] [PDF]

				D. Muller, S. J. Kugler, A. Preiss, D. Maier, and A. C. Nagel Genetic Modifier Screens on Hairless Gain-of-Function Phenotypes Reveal Genes Involved in Cell Differentiation, Cell Growth and Apoptosis in Drosophila melanogaster Genetics, November 1, 2005; 171(3): 1137 - 1152. [Abstract] [Full Text] [PDF]

				A. Singh, J. Chan, J. J. Chern, and K.-W. Choi Genetic Interaction of Lobe With Its Modifiers in Dorsoventral Patterning and Growth of the Drosophila Eye Genetics, September 1, 2005; 171(1): 169 - 183. [Abstract] [Full Text] [PDF]

				M. E. Clark, C. L. Anderson, J. Cande, and T. L. Karr Widespread Prevalence of Wolbachia in Laboratory Stocks and the Implications for Drosophila Research Genetics, August 1, 2005; 170(4): 1667 - 1675. [Abstract] [Full Text] [PDF]

				M. J. Laviolette, P. Nunes, J.-B. Peyre, T. Aigaki, and B. A. Stewart A Genetic Screen for Suppressors of Drosophila NSF2 Neuromuscular Junction Overgrowth Genetics, June 1, 2005; 170(2): 779 - 792. [Abstract] [Full Text] [PDF]

				S. Beronja, P. Laprise, O. Papoulas, M. Pellikka, J. Sisson, and U. Tepass Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells J. Cell Biol., May 23, 2005; 169(4): 635 - 646. [Abstract] [Full Text] [PDF]

				N. Haines and K. D. Irvine Functional analysis of Drosophila {beta}1,4-N-acetlygalactosaminyltransferases Glycobiology, April 1, 2005; 15(4): 335 - 346. [Abstract] [Full Text] [PDF]

				B. van Swinderen and R. J. Greenspan Flexibility in a Gene Network Affecting a Simple Behavior in Drosophila melanogaster Genetics, April 1, 2005; 169(4): 2151 - 2163. [Abstract] [Full Text] [PDF]

				J. C. Greene, A. J. Whitworth, L. A. Andrews, T. J. Parker, and L. J. Pallanck Genetic and genomic studies of Drosophila parkin mutants implicate oxidative stress and innate immune responses in pathogenesis Hum. Mol. Genet., March 15, 2005; 14(6): 799 - 811. [Abstract] [Full Text] [PDF]

				K. Weber, N. Johnson, D. Champlin, and A. Patty Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster Genetics, March 1, 2005; 169(3): 1461 - 1475. [Abstract] [Full Text] [PDF]

				M. Rosenzweig, K. M. Brennan, T. D. Tayler, P. O. Phelps, A. Patapoutian, and P. A. Garrity The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis Genes & Dev., February 15, 2005; 19(4): 419 - 424. [Abstract] [Full Text] [PDF]

				G.-C. Chen, B. Turano, P. J. Ruest, M. Hagel, J. Settleman, and S. M. Thomas Regulation of Rho and Rac Signaling to the Actin Cytoskeleton by Paxillin during Drosophila Development Mol. Cell. Biol., February 1, 2005; 25(3): 979 - 987. [Abstract] [Full Text] [PDF]

				A. Pascual, K.-L. Huang, and T. Preat Conditional UAS-targeted repression in Drosophila Nucleic Acids Res., January 13, 2005; 33(1): e7 - e7. [Abstract] [Full Text] [PDF]

				J. C. Adam and D. J. Montell A role for extra macrochaetae downstream of Notch in follicle cell differentiation Development, December 1, 2004; 131(23): 5971 - 5980. [Abstract] [Full Text] [PDF]

				N. Yan and P. M. Macdonald Genetic Interactions of Drosophila melanogaster arrest Reveal Roles for Translational Repressor Bruno in Accumulation of Gurken and Activity of Delta Genetics, November 1, 2004; 168(3): 1433 - 1442. [Abstract] [Full Text] [PDF]

				H. Eggert, A. Gortchakov, and H. Saumweber Identification of the Drosophila interband-specific protein Z4 as a DNA-binding zinc-finger protein determining chromosomal structure J. Cell Sci., August 15, 2004; 117(18): 4253 - 4264. [Abstract] [Full Text] [PDF]

				K. Okamura, A. Ishizuka, H. Siomi, and M. C. Siomi Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways Genes & Dev., July 15, 2004; 18(14): 1655 - 1666. [Abstract] [Full Text] [PDF]

				D. W. Walker and S. Benzer From the Cover: Mitochondrial "swirls" induced by oxygen stress and in the Drosophila mutant hyperswirl PNAS, July 13, 2004; 101(28): 10290 - 10295. [Abstract] [Full Text] [PDF]

				K. Raymond, E. Bergeret, A. Avet-Rochex, R. Griffin-Shea, and M.-O. Fauvarque A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis J. Cell Sci., June 1, 2004; 117(13): 2777 - 2789. [Abstract] [Full Text] [PDF]

				C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood, and M. T. Fuller A Misexpression Screen Reveals Effects of bag-of-marbles and TGF{beta} Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage Genetics, June 1, 2004; 167(2): 707 - 723. [Abstract] [Full Text] [PDF]

				H. J. Bellen, R. W. Levis, G. Liao, Y. He, J. W. Carlson, G. Tsang, M. Evans-Holm, P. R. Hiesinger, K. L. Schulze, G. M. Rubin, et al. The BDGP Gene Disruption Project: Single Transposon Insertions Associated With 40% of Drosophila Genes Genetics, June 1, 2004; 167(2): 761 - 781. [Abstract] [Full Text] [PDF]

				L. S. Collier, K. Suyama, J. H. Anderson, and M. P. Scott Drosophila Costal1 Mutations Are Alleles of Protein Kinase A That Modulate Hedgehog Signaling Genetics, June 1, 2004; 167(2): 783 - 796. [Abstract] [Full Text] [PDF]

				P. Szafranski and S. Goode A Fasciclin 2 morphogenetic switch organizes epithelial cell cluster polarity and motility Development, May 1, 2004; 131(9): 2023 - 2036. [Abstract] [Full Text] [PDF]

				S. E. McGuire, Z. Mao, and R. L. Davis Spatiotemporal Gene Expression Targeting with the TARGET and Gene-Switch Systems in Drosophila Sci. Signal., February 17, 2004; 2004(220): pl6 - pl6. [Abstract] [Full Text] [PDF]

				A. Y. Konev, C. M. Yan, D. Acevedo, C. Kennedy, E. Ward, A. Lim, S. Tickoo, and G. H. Karpen Genetics of P-Element Transposition Into Drosophila melanogaster Centric Heterochromatin Genetics, December 1, 2003; 165(4): 2039 - 2053. [Abstract] [Full Text] [PDF]

				S. S. Blair Genetic mosaic techniques for studying Drosophila development Development, November 1, 2003; 130(21): 5065 - 5072. [Abstract] [Full Text] [PDF]

				J. M. Shulman and M. B. Feany Genetic Modifiers of Tauopathy in Drosophila Genetics, November 1, 2003; 165(3): 1233 - 1242. [Abstract] [Full Text] [PDF]

				W. Zhang, Y. Jin, Y. Ji, J. Girton, J. Johansen, and K. M. Johansen Genetic and Phenotypic Analysis of Alleles of the Drosophila Chromosomal JIL-1 Kinase Reveals a Functional Requirement at Multiple Developmental Stages Genetics, November 1, 2003; 165(3): 1341 - 1354. [Abstract] [Full Text] [PDF]

				P. H. Patel, N. Thapar, L. Guo, M. Martinez, J. Maris, C.-L. Gau, J. A. Lengyel, and F. Tamanoi Drosophila Rheb GTPase is required for cell cycle progression and cell growth J. Cell Sci., September 1, 2003; 116(17): 3601 - 3610. [Abstract] [Full Text] [PDF]

				J. A. McDonald, E. M. Pinheiro, and D. J. Montell PVF1, a PDGF/VEGF homolog, is sufficient to guide border cells and interacts genetically with Taiman Development, August 1, 2003; 130(15): 3469 - 3478. [Abstract] [Full Text] [PDF]

				F. Rintelen, E. Hafen, and K. Nairz The Drosophila dual-specificity ERK phosphatase DMKP3 cooperates with the ERK tyrosine phosphatase PTP-ER Development, August 1, 2003; 130(15): 3479 - 3490. [Abstract] [Full Text] [PDF]

				D. J. van Meyel, J. B. Thomas, and A. D. Agulnick Ssdp proteins bind to LIM-interacting co-factors and regulate the activity of LIM-homeodomain protein complexes in vivo Development, May 1, 2003; 130(9): 1915 - 1925. [Abstract] [Full Text] [PDF]

				L. Cherbas, X. Hu, I. Zhimulev, E. Belyaeva, and P. Cherbas EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue Development, March 2, 2003; 130(2): 271 - 284. [Abstract] [Full Text] [PDF]

				L. Gutierrez, M. Zurita, J. A. Kennison, and M. Vazquez The Drosophila trithorax group gene tonalli(tna) interacts genetically with the Brahma remodeling complex and encodes an SP-RING finger protein Development, March 2, 2003; 130(2): 343 - 354. [Abstract] [Full Text] [PDF]

				A. Pacquelet, L. Lin, and P. Rorth Binding site for p120/{delta}-catenin is not required for Drosophila E-cadherin function in vivo J. Cell Biol., February 3, 2003; 160(3): 313 - 319. [Abstract] [Full Text] [PDF]

				G. Mirey, M. Balakireva, S. L'Hoste, C. Rosse, S. Voegeling, and J. Camonis A Ral Guanine Exchange Factor-Ral Pathway Is Conserved in Drosophila melanogaster and Sheds New Light on the Connectivity of the Ral, Ras, and Rap Pathways Mol. Cell. Biol., February 1, 2003; 23(3): 1112 - 1124. [Abstract] [Full Text]

				T. F. Satterfield, S. M. Jackson, and L. J. Pallanck A Drosophila Homolog of the Polyglutamine Disease Gene SCA2 Is a Dosage-Sensitive Regulator of Actin Filament Formation Genetics, December 1, 2002; 162(4): 1687 - 1702. [Abstract] [Full Text] [PDF]

				C. Maurange and R. Paro A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development Genes & Dev., October 15, 2002; 16(20): 2672 - 2683. [Abstract] [Full Text] [PDF]

				S. E. Mohr and W. M. Gelbart Using the P{wHy} Hybrid Transposable Element to Disrupt Genes in Region 54D-55B in Drosophila melanogaster Genetics, September 1, 2002; 162(1): 165 - 176. [Abstract] [Full Text] [PDF]

				A.-S. K. Tseng and I. K. Hariharan An Overexpression Screen in Drosophila for Genes That Restrict Growth or Cell-Cycle Progression in the Developing Eye Genetics, September 1, 2002; 162(1): 229 - 243. [Abstract] [Full Text] [PDF]

				K. Nairz, H. Stocker, B. Schindelholz, and E. Hafen High-resolution SNP mapping by denaturing HPLC PNAS, August 6, 2002; 99(16): 10575 - 10580. [Abstract] [Full Text] [PDF]

				R. Dorfman, L. Glazer, U. Weihe, M. F. Wernet, and B.-Z. Shilo Elbow and Noc define a family of zinc finger proteins controlling morphogenesis of specific tracheal branches Development, August 1, 2002; 129(15): 3585 - 3596. [Abstract] [Full Text] [PDF]

				D. R. Hipfner, K. Weigmann, and S. M. Cohen The bantam Gene Regulates Drosophila Growth Genetics, August 1, 2002; 161(4): 1527 - 1537. [Abstract] [Full Text] [PDF]

				S. Gorski and M. Marra Programmed cell death takes flight: genetic and genomic approaches to gene discovery in Drosophila Physiol Genomics, May 10, 2002; 9(2): 59 - 69. [Abstract] [Full Text] [PDF]

				K. L. Giunta, J. K. Jang, E. A. Manheim, G. Subramanian, and K. S. McKim subito Encodes a Kinesin-like Protein Required for Meiotic Spindle Pole Formation in Drosophila melanogaster Genetics, April 1, 2002; 160(4): 1489 - 1501. [Abstract] [Full Text] [PDF]

				S. E. Mohr and R. E. Boswell Genetic Analysis of Drosophila melanogaster Polytene Chromosome Region 44D-45F: Loci Required for Viability and Fertility Genetics, April 1, 2002; 160(4): 1503 - 1510. [Abstract] [Full Text] [PDF]

				M. Buszczak, X. Lu, W. A. Segraves, T. Y. Chang, and L. Cooley Mutations in the midway Gene Disrupt a Drosophila Acyl Coenzyme A: Diacylglycerol Acyltransferase Genetics, April 1, 2002; 160(4): 1511 - 1518. [Abstract] [Full Text] [PDF]

				W. Chen, S. Burgess, G. Golling, A. Amsterdam, and N. Hopkins High-Throughput Selection of Retrovirus Producer Cell Lines Leads to Markedly Improved Efficiency of Germ Line-Transmissible Insertions in Zebra Fish J. Virol., March 1, 2002; 76(5): 2192 - 2198. [Abstract] [Full Text] [PDF]

				B. Timakov, X. Liu, I. Turgut, and P. Zhang Timing and Targeting of P-Element Local Transposition in the Male Germline Cells of Drosophila melanogaster Genetics, March 1, 2002; 160(3): 1011 - 1022. [Abstract] [Full Text] [PDF]

				M. T. Pena-Rangel, I. Rodriguez, and J. R. Riesgo-Escovar A Misexpression Study Examining Dorsal Thorax Formation in Drosophila melanogaster Genetics, March 1, 2002; 160(3): 1035 - 1050. [Abstract] [Full Text] [PDF]

				N. Aulner, C. Monod, G. Mandicourt, D. Jullien, O. Cuvier, A. Sall, S. Janssen, U. K. Laemmli, and E. Kas The AT-Hook Protein D1 Is Essential for Drosophila melanogaster Development and Is Implicated in Position-Effect Variegation Mol. Cell. Biol., February 15, 2002; 22(4): 1218 - 1232. [Abstract] [Full Text] [PDF]

				G. Schwed, N. May, Y. Pechersky, and B. R. Calvi Drosophila Minichromosome Maintenance 6 Is Required for Chorion Gene Amplification and Genomic Replication Mol. Biol. Cell, February 1, 2002; 13(2): 607 - 620. [Abstract] [Full Text] [PDF]

				S. M. Jackson and C. A. Berg An A-kinase anchoring protein is required for Protein kinase A regulatory subunit localization and morphology of actin structures during oogenesis in Drosophila Development, January 10, 2002; 129(19): 4423 - 4433. [Abstract] [Full Text] [PDF]

				J. H. Mansfield, J. E. Wilhelm, and T. Hazelrigg Ypsilon Schachtel, a Drosophila Y-box protein, acts antagonistically to Orb in the oskar mRNA localization and translation pathway Development, January 1, 2002; 129(1): 197 - 209. [Abstract] [Full Text] [PDF]

				A. C. Zelhof, H. Bao, R. W. Hardy, A. Razzaq, B. Zhang, and C. Q. Doe Drosophila Amphiphysin is implicated in protein localization and membrane morphogenesis but not in synaptic vesicle endocytosis Development, December 15, 2001; 128(24): 5005 - 5015. [Abstract] [Full Text] [PDF]

				H. A. Dworak, M. A. Charles, L. B. Pellerano, and H. Sink Characterization of Drosophila hibris, a gene related to human nephrin Development, November 1, 2001; 128(21): 4265 - 4276. [Abstract] [Full Text] [PDF]

				T. Osterwalder, K. S. Yoon, B. H. White, and H. Keshishian A conditional tissue-specific transgene expression system using inducible GAL4 PNAS, October 23, 2001; 98(22): 12596 - 12601. [Abstract] [Full Text] [PDF]

				C. M. Bergman and M. Kreitman Analysis of Conserved Noncoding DNA in Drosophila Reveals Similar Constraints in Intergenic and Intronic Sequences Genome Res., August 1, 2001; 11(8): 1335 - 1345. [Abstract] [Full Text] [PDF]

				G. Landis, D. Bhole, L. Lu, and J. Tower High-Frequency Generation of Conditional Mutations Affecting Drosophila melanogaster Development and Life Span Genetics, July 1, 2001; 158(3): 1167 - 1176. [Abstract] [Full Text] [PDF]

				P. V. Benos, M. K. Gatt, L. Murphy, D. Harris, B. Barrell, C. Ferraz, S. Vidal, C. Brun, J. Demaille, E. Cadieu, et al. From First Base: The Sequence of the Tip of the X Chromosome of Drosophila melanogaster, a Comparison of Two Sequencing Strategies Genome Res., May 1, 2001; 11(5): 710 - 730. [Abstract] [Full Text]

				K. Afshar, P. Gönczy, S. DiNardo, and S. A. Wasserman fumble Encodes a Pantothenate Kinase Homolog Required for Proper Mitosis and Meiosis in Drosophila melanogaster Genetics, March 1, 2001; 157(3): 1267 - 1276. [Abstract] [Full Text]

				T. Lukacsovich, Z. Asztalos, W. Awano, K. Baba, S. Kondo, S. Niwa, and D. Yamamoto Dual-Tagging Gene Trap of Novel Genes in Drosophila melanogaster Genetics, February 1, 2001; 157(2): 727 - 742. [Abstract] [Full Text]

				M Starz-Gaiano, N. Cho, A Forbes, and R Lehmann Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells Development, January 3, 2001; 128(6): 983 - 991. [Abstract] [PDF]

				S. N. Prokopenko, Y. He, Y. Lu, and H. J. Bellen Mutations Affecting the Development of the Peripheral Nervous System in Drosophila: A Molecular Screen for Novel Proteins Genetics, December 1, 2000; 156(4): 1691 - 1715. [Abstract] [Full Text]

				L. Wan, T. C. Dockendorff, T. A. Jongens, and G. Dreyfuss Characterization of dFMR1, a Drosophila melanogaster Homolog of the Fragile X Mental Retardation Protein Mol. Cell. Biol., November 15, 2000; 20(22): 8536 - 8547. [Abstract] [Full Text]

				S. Oldham, J. Montagne, T. Radimerski, G. Thomas, and E. Hafen Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin Genes & Dev., November 1, 2000; 14(21): 2689 - 2694. [Abstract] [Full Text]

				F. Chanut, A. Luk, and U. Heberlein A Screen for Dominant Modifiers of roDom, a Mutation That Disrupts Morphogenetic Furrow Progression in Drosophila, Identifies Groucho and Hairless as Regulators of atonal Expression Genetics, November 1, 2000; 156(3): 1203 - 1217. [Abstract] [Full Text]

				A. M. Huang and G. M. Rubin A Misexpression Screen Identifies Genes That Can Modulate RAS1 Pathway Signaling in Drosophila melanogaster Genetics, November 1, 2000; 156(3): 1219 - 1230. [Abstract] [Full Text]

				C. F. Cullen, K. M. May, I. M. Hagan, D. M. Glover, and H. Ohkura A New Genetic Method for Isolating Functionally Interacting Genes: High plo1+-Dependent Mutants and Their Suppressors Define Genes in Mitotic and Septation Pathways in Fission Yeast Genetics, August 1, 2000; 155(4): 1521 - 1534. [Abstract] [Full Text]

				S. L. Page, K. S. McKim, B. Deneen, T. L. Van Hook, and R. S. Hawley Genetic Studies of mei-P26 Reveal a Link Between the Processes That Control Germ Cell Proliferation in Both Sexes and Those That Control Meiotic Exchange in Drosophila Genetics, August 1, 2000; 155(4): 1757 - 1772. [Abstract] [Full Text]