|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 7 1261-1267, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
S Misra, P Hecht, R Maeda and KV Anderson
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
The sequential activities of four members of the trypsin family of extracellular serine proteases are required for the production of the ventrally localized ligand that organizes the dorsal-ventral pattern of the Drosophila embryo. The last protease in this sequence is encoded by easter, which is a candidate to activate proteolytically the ligand encoded by spatzle. Here, we demonstrate biochemically that the zymogen form of Easter is processed in vivo by a proteolytic cleavage event that requires the three upstream proteases. Processed Easter is present in extremely low amounts in the early embryo because it is rapidly converted into a high molecular mass complex, which may contain a protease inhibitor. Easter zymogen activation is also controlled by a negative feedback loop from Dorsal, the transcription factor at the end of the signaling pathway. Each of these regulated biochemical processes is likely to be important in generating the ventral-to-dorsal gradient of Dorsal protein that organizes cell fates in the early embryo.
This article has been cited by other articles:
|
|
 |
|
  D. M. de Groot and G. J. M. Martens Expression of Neuroserpin Is Linked to Neuroendocrine Cell Activation Endocrinology, September 1, 2005; 146(9): 3791 - 3799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, B. Ho, and J. L. Ding Transcriptional Regulation of Limulus Factor C: REPRESSION OF AN NF{kappa}B MOTIF MODULATES ITS RESPONSIVENESS TO BACTERIAL LIPOPOLYSACCHARIDE J. Biol. Chem., December 5, 2003; 278(49): 49428 - 49437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Chang and D. Morisato Regulation of Easter activity is required for shaping the Dorsal gradient in the Drosophila embryo Development, March 14, 2003; 129(24): 5635 - 5645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Peri, M. Technau, and S. Roth Mechanisms of Gurken-dependent pipe regulation and the robustness of dorsoventral patterning in Drosophila Development, March 8, 2003; 129(12): 2965 - 2975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Towb, A. Bergmann, and S. A. Wasserman The protein kinase Pelle mediates feedback regulation in the Drosophila Toll signaling pathway Development, December 1, 2001; 128(23): 4729 - 4736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Han, S. H. Lee, Y.-Q. Tan, E. K. LeMosy, and C. Hashimoto Gastrulation Defective is a serine protease involved in activating the receptor Toll to polarize the Drosophila embryo PNAS, August 1, 2000; 97(16): 9093 - 9097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Chen, K Handel, and S Roth The maternal NF-kappaB/dorsal gradient of Tribolium castaneum: dynamics of early dorsoventral patterning in a short-germ beetle Development, January 12, 2000; 127(23): 5145 - 5156. [Abstract] [PDF]
|
|
|
|
|
|
L. L. Lindsay, J. C. Yang, and J. L. Hedrick Ovochymase, a Xenopus laevis egg extracellular protease, is translated as part of an unusual polyprotease PNAS, September 28, 1999; 96(20): 11253 - 11258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Groover and A. M. Jones Tracheary Element Differentiation Uses a Novel Mechanism Coordinating Programmed Cell Death and Secondary Cell Wall Synthesis Plant Physiology, February 1, 1999; 119(2): 375 - 384. [Abstract] [Full Text]
|
|
|
|
|
|
S Roth, P Jordan, and R Karess Binuclear Drosophila oocytes: consequences and implications for dorsal-ventral patterning in oogenesis and embryogenesis Development, January 2, 1999; 126(5): 927 - 934. [Abstract] [PDF]
|
|
|
|
|
|
E. LeMosy, D Kemler, and C Hashimoto Role of Nudel protease activation in triggering dorsoventral polarization of the Drosophila embryo Development, January 10, 1998; 125(20): 4045 - 4053. [Abstract] [PDF]
|
|
|
|
|
|
E. K. LeMosy, Y.-Q. Tan, and C. Hashimoto Activation of a protease cascade involved in patterning the Drosophila embryo PNAS, April 24, 2001; 98(9): 5055 - 5060. [Abstract] [Full Text] [PDF]
|
|
