|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 8 1519-1528, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
P Das, LL Maduzia, H Wang, AL Finelli, SH Cho, MM Smith and RW Padgett
Waksman Institute, Department of Molecular Biology, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA.
Signals from transforming growth factor-beta (TGF-beta) ligands are transmitted within the cell by members of the Smad family, which can be grouped into three classes based on sequence similarities. Our previous identification of both class I and II Smads functioning in a single pathway in C. elegans, raised the issue of whether the requirement for Smads derived from different classes is a general feature of TGF-beta signaling. We report here the identification of a new Drosophila class II Smad, Medea, a close homolog of the human tumor-suppressor gene DPC4. Embryos from germline clones of both Medea and Mad (a class I Smad) are ventralized, as are embryos null for the TGF-beta-like ligand decapentaplegic (dpp). Loss of Medea also blocks dpp signaling during later development, suggesting that Medea, like Mad, is universally required for dpp signaling. Furthermore, we show that the necessity for these two closely related, non-redundant Smads, is due to their different signaling properties - upon activation of the Dpp pathway, Mad is required to actively translocate Medea into the nucleus. These results provide a paradigm for, and distinguish between, the requirement for class I and II Smads in Dpp/BMP signaling.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Kahlem and S. J. Newfeld Informatics approaches to understanding TGF{beta} pathway regulation Development, November 15, 2009; 136(22): 3729 - 3740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yao, X. Chen, C. Cottonham, and L. Xu Preferential Utilization of Imp7/8 in Nuclear Import of Smads J. Biol. Chem., August 15, 2008; 283(33): 22867 - 22874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Lorenzen, A. Gnirke, J. Margolis, J. Garnes, M. Campbell, J. J. Stuart, R. Aggarwal, S. Richards, Y. Park, and R. W. Beeman The maternal-effect, selfish genetic element Medea is associated with a composite Tc1 transposon PNAS, July 22, 2008; 105(29): 10085 - 10089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Walsh and S. B. Carroll Collaboration between Smads and a Hox protein in target gene repression Development, October 15, 2007; 134(20): 3585 - 3592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu, X. Yao, X. Chen, P. Lu, B. Zhang, and Y. T. Ip Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads J. Cell Biol., September 7, 2007; 178(6): 981 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. de Navascues and J. Modolell tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification Development, May 1, 2007; 134(9): 1779 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, W. X. Li, and W. M. Gelbart A Genetic Screen for Maternal-Effect Suppressors of decapentaplegic Identifies the eukaryotic translation initiation factor 4A in Drosophila Genetics, December 1, 2005; 171(4): 1629 - 1641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. T. Takaesu, E. Herbig, D. Zhitomersky, M. B. O'Connor, and S. J. Newfeld DNA-binding domain mutations in SMAD genes yield dominant-negative proteins or a neomorphic protein that can activate WG target genes in Drosophila Development, November 1, 2005; 132(21): 4883 - 4894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yoshida, L. Soustelle, A. Giangrande, D. Umetsu, S. Murakami, T. Yasugi, T. Awasaki, K. Ito, M. Sato, and T. Tabata DPP signaling controls development of the lamina glia required for retinal axon targeting in the visual system of Drosophila Development, October 15, 2005; 132(20): 4587 - 4598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Chu, N. R. Dunn, D. C. Anderson, L. Oxburgh, and E. J. Robertson Differential requirements for Smad4 in TGF{beta}-dependent patterning of the early mouse embryo Development, August 1, 2004; 131(15): 3501 - 3512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Song, M. D. Wong, E. Kawase, R. Xi, B. C. Ding, J. J. McCarthy, and T. Xie Bmp signals from niche cells directly repress transcription of a differentiation-promoting gene, bag of marbles, in germline stem cells in the Drosophila ovary Development, March 15, 2004; 131(6): 1353 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kawase, M. D. Wong, B. C. Ding, and T. Xie Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis Development, March 15, 2004; 131(6): 1365 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Janody, J. D. Lee, N. Jahren, D. J. Hazelett, A. Benlali, G. I. Miura, I. Draskovic, and J. E. Treisman A Mosaic Genetic Screen Reveals Distinct Roles for trithorax and Polycomb Group Genes in Drosophila Eye Development Genetics, January 1, 2004; 166(1): 187 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Sutherland, M. Li, X.-q. Liu, R. Stefancsik, and L. A. Raftery Stepwise formation of a SMAD activity gradient during dorsal-ventral patterning of the Drosophila embryo Development, December 1, 2003; 130(23): 5705 - 5716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Zhu and T. Xie Clonal expansion of ovarian germline stem cells during niche formation in Drosophila Development, June 15, 2003; 130(12): 2579 - 2588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chang, C. W. Brown, and M. M. Matzuk Genetic Analysis of the Mammalian Transforming Growth Factor-{beta} Superfamily Endocr. Rev., December 1, 2002; 23(6): 787 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Newfeld and N. T. Takaesu An Analysis Using the hobo Genetic System Reveals That Combinatorial Signaling by the Dpp and Wg Pathways Regulates dpp Expression in Leading Edge Cells of the Dorsal Ectoderm in Drosophila melanogaster Genetics, June 1, 2002; 161(2): 685 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baonza and M. Freeman Notch signalling and the initiation of neural development in the Drosophila eye Development, October 15, 2001; 128(20): 3889 - 3898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Decotto and E. L. Ferguson A positive role for Short gastrulation in modulating BMP signaling during dorsoventral patterning in the Drosophila embryo Development, October 1, 2001; 128(19): 3831 - 3841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Torres-Vazquez, S Park, R Warrior, and K Arora The transcription factor Schnurri plays a dual role in mediating Dpp signaling during embryogenesis Development, January 5, 2001; 128(9): 1657 - 1670. [Abstract] [PDF]
|
|
|
|
|
|
F Peri and S Roth Combined activities of Gurken and decapentaplegic specify dorsal chorion structures of the Drosophila egg Development, January 2, 2000; 127(4): 841 - 850. [Abstract] [PDF]
|
|
|
|
 |
|
 E. PIEK, C.-H. HELDIN, and P. TEN DIJKE Specificity, diversity, and regulation in TGF-{beta} superfamily signaling FASEB J, December 1, 1999; 13(15): 2105 - 2124. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Wharton, J. M. Cook, S. Torres-Schumann, K. de Castro, E. Borod, and D. A. Phillips Genetic Analysis of the Bone Morphogenetic Protein-Related Gene, gbb, Identifies Multiple Requirements During Drosophila Development Genetics, June 1, 1999; 152(2): 629 - 640. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Adachi-Yamada, M. Nakamura, K. Irie, Y. Tomoyasu, Y. Sano, E. Mori, S. Goto, N. Ueno, Y. Nishida, and K. Matsumoto p38 Mitogen-Activated Protein Kinase Can Be Involved in Transforming Growth Factor beta Superfamily Signal Transduction in Drosophila Wing Morphogenesis Mol. Cell. Biol., March 1, 1999; 19(3): 2322 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-G. Chen and J. Massague Smad1 Recognition and Activation by the ALK1 Group of Transforming Growth Factor-beta Family Receptors J. Biol. Chem., February 5, 1999; 274(6): 3672 - 3677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Jazwinska, C Rushlow, and S Roth The role of brinker in mediating the graded response to Dpp in early Drosophila embryos Development, January 8, 1999; 126(15): 3323 - 3334. [Abstract] [PDF]
|
|
|
|
|
|
M Hild, A Dick, G. Rauch, A Meier, T Bouwmeester, P Haffter, and M Hammerschmidt The smad5 mutation somitabun blocks Bmp2b signaling during early dorsoventral patterning of the zebrafish embryo Development, January 5, 1999; 126(10): 2149 - 2159. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Brummel, S. Abdollah, T. E. Haerry, M. J. Shimell, J. Merriam, L. Raftery, J. L. Wrana, and M. B. O'Connor The Drosophila Activin receptor Baboon signals through dSmad2 and controls cell proliferation but not patterning during larval development Genes & Dev., January 1, 1999; 13(1): 98 - 111. [Abstract] [Full Text]
|
|
|
|
|
|
C. Pouponnot, L. Jayaraman, and J. Massague Physical and Functional Interaction of SMADs and p300/CBP J. Biol. Chem., September 4, 1998; 273(36): 22865 - 22868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Inoue, T. Imamura, Y. Ishidou, M. Takase, Y. Udagawa, Y. Oka, K. Tsuneizumi, T. Tabata, K. Miyazono, and M. Kawabata Interplay of Signal Mediators of Decapentaplegic (Dpp): Molecular Characterization of Mothers against dpp, Medea, and Daughters against dpp Mol. Biol. Cell, August 1, 1998; 9(8): 2145 - 2156. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kirkpatrick, K. Johnson, and A. Laughon Repression of Dpp Targets by Binding of Brinker to Mad Sites J. Biol. Chem., May 18, 2001; 276(21): 18216 - 18222. [Abstract] [Full Text] [PDF]
|
|
