|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 9 1679-1689, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
TA Bunch, MW Graner, LI Fessler, JH Fessler, KD Schneider, A Kerschen, LP Choy, BW Burgess and DL Brower
University of Arizona, Department of Molecular Biology, Tuscon, Arizona 85721, USA. tbunch@u.arizona.edu
Tiggrin is a novel extracellular matrix ligand for the Drosophila PS2 integrins. We have used flanking P elements to generate a precise deletion of tiggrin. Most flies lacking tiggrin die as larvae or pupae. A few adults do emerge and these appear to be relatively normal, displaying only misshapen abdomens and a low frequency of wing defects. Examination of larvae shows that muscle connections, function and morphology are defective in tiggrin mutants. Muscle contraction waves that extend the length of the larvae are much slower in tiggrin mutants. Direct examination of bodywall muscles shows defects in muscle attachment sites, where tiggrin is specifically localized, and muscles appear thinner. Transgenes expressing tiggrin are capable of rescuing tiggrin mutant phenotypes. Transgenes expressing a mutant tiggrin, whose Arg-Gly-Asp (RGD) integrin recognition sequence has been mutated to Leu-Gly-Ala (LGA) show much reduced, but significant, rescuing ability. Cell spreading assays detect no interactions of this mutant tiggrin with PS2 integrins. Therefore, while the RGD sequence is critical for PS2 interactions and full activity in the whole fly, the mutant tiggrin retains some function(s) that are probably mediated by interactions with other ECM molecules or cell surface receptors
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Zhang, Y. Zhang, and K. G. T. Hagen A Mucin-type O-Glycosyltransferase Modulates Cell Adhesion during Drosophila Development J. Biol. Chem., December 5, 2008; 283(49): 34076 - 34086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Kocherlakota, J.-m. Wu, J. McDermott, and S. M. Abmayr Analysis of the Cell Adhesion Molecule Sticks-and-Stones Reveals Multiple Redundant Functional Domains, Protein-Interaction Motifs and Phosphorylated Tyrosines That Direct Myoblast Fusion in Drosophila melanogaster Genetics, March 1, 2008; 178(3): 1371 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Subramanian, B. Wayburn, T. Bunch, and T. Volk Thrombospondin-mediated adhesion is essential for the formation of the myotendinous junction in Drosophila Development, April 1, 2007; 134(7): 1269 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Bunch, T. L. Helsten, T. L. Kendall, N. Shirahatti, D. Mahadevan, S. J. Shattil, and D. L. Brower Amino Acid Changes in Drosophila {alpha}PS2betaPS Integrins That Affect Ligand Affinity J. Biol. Chem., February 24, 2006; 281(8): 5050 - 5057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. P. Devine, B. Lubarsky, K. Shaw, S. Luschnig, L. Messina, and M. A. Krasnow Requirement for chitin biosynthesis in epithelial tube morphogenesis PNAS, November 22, 2005; 102(47): 17014 - 17019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grabbe, C. G. Zervas, T. Hunter, N. H. Brown, and R. H. Palmer Focal adhesion kinase is not required for integrin function or viability in Drosophila Development, December 1, 2004; 131(23): 5795 - 5805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Soustelle, C. Jacques, B. Altenhein, G. M. Technau, T. Volk, and A. Giangrande Terminal tendon cell differentiation requires the glide/gcm complex Development, September 15, 2004; 131(18): 4521 - 4532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Jannuzi, T. A. Bunch, R. F. West, and D. L. Brower Identification of Integrin {beta} Subunit Mutations that Alter Heterodimer Function In Situ Mol. Biol. Cell, August 1, 2004; 15(8): 3829 - 3840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Swan, C. Wichmann, U. Prange, A. Schmid, M. Schmidt, T. Schwarz, E. Ponimaskin, F. Madeo, G. Vorbruggen, and S. J. Sigrist A Glutamate Receptor-Interacting Protein homolog organizes muscle guidance in Drosophila Genes & Dev., January 15, 2004; 18(2): 223 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wang, K. M. C. Sullivan, and K. Beckingham Drosophila Calmodulin Mutants With Specific Defects in the Musculature or in the Nervous System Genetics, November 1, 2003; 165(3): 1255 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Stein, H. T. Broihier, L. A. Moore, and R. Lehmann Slow as Molasses is required for polarized membrane growth and germ cell migration in Drosophila Development, March 10, 2003; 129(16): 3925 - 3934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Llano, G. Adam, A. M. Pendas, V. Quesada, L. M. Sanchez, I. Santamaria, S. Noselli, and C. Lopez-Otin Structural and Enzymatic Characterization of Drosophila Dm2-MMP, a Membrane-bound Matrix Metalloproteinase with Tissue-specific Expression J. Biol. Chem., June 21, 2002; 277(26): 23321 - 23329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Stevens and J. R. Jacobs Integrins Regulate Responsiveness to Slit Repellent Signals J. Neurosci., June 1, 2002; 22(11): 4448 - 4455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Korey, G. Wilkie, I. Davis, and D. Van Vactor small bristles Is Required for the Morphogenesis of Multiple Tissues During Drosophila Development Genetics, December 1, 2001; 159(4): 1659 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang, J. Li, Y. Zhang, and C. Wu The Roles of Integrin-Linked Kinase in the Regulation of Myogenic Differentiation J. Cell Biol., August 21, 2000; 150(4): 861 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Martin-Bermudo and N. Brown The localized assembly of extracellular matrix integrin ligands requires cell-cell contact J. Cell Sci., January 11, 2000; 113(21): 3715 - 3723. [Abstract] [PDF]
|
|
|
|
|
|
R. H. Palmer, L. I. Fessler, P. T. Edeen, S. J. Madigan, M. McKeown, and T. Hunter DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase J. Biol. Chem., December 10, 1999; 274(50): 35621 - 35629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fujimoto, K. Sawamoto, M. Okabe, Y. Takagi, T. Tezuka, S. Yoshikawa, H. Ryo, H. Okano, and T. Yamamoto Cloning and Characterization of Dfak56, a Homolog of Focal Adhesion Kinase, in Drosophila melanogaster J. Biol. Chem., October 8, 1999; 274(41): 29196 - 29201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hoang and A. Chiba Genetic Analysis on the Role of Integrin during Axon Guidance in Drosophila J. Neurosci., October 1, 1998; 18(19): 7847 - 7855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Walsh and N. H. Brown A Screen to Identify Drosophila Genes Required for Integrin-Mediated Adhesion Genetics, October 1, 1998; 150(2): 791 - 805. [Abstract] [Full Text]
|
|
|
|
|
|
M. W. Graner, T. A. Bunch, S. Baumgartner, A. Kerschen, and D. L. Brower Splice Variants of the Drosophila PS2 Integrins Differentially Interact with RGD-containing Fragments of the Extracellular Proteins Tiggrin, Ten-m, and D-Laminin alpha 2 J. Biol. Chem., July 17, 1998; 273(29): 18235 - 18241. [Abstract] [Full Text] [PDF]
|
|
