|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 1 119-125, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
B Christen and JM Slack
Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
We have examined the spatial pattern of activation of the extracellular signal-regulated protein kinase (ERK) during Xenopus development, and show that it closely resembles the expression of various fibroblast growth factors (FGFs). Until the tailbud stage of development, all ERK activation domains are sensitive to the dominant negative FGF receptor, showing that activation is generated by endogenous FGF signalling. ERK is not activated by application of other growth factors like BMP4 or activin, nor is endogenous activation blocked by the respective dominant negative receptors. This shows that various domains of FGF expression, including the periblastoporal region and the midbrain-hindbrain boundary, are also sites of FGF signalling in vivo. Wounding induces a transient (<60 minutes) activation of ERK which is not significantly reduced by the dominant negative FGF receptor. An artificial FGF source, created by injection of eFGF mRNA into cleavage stage embryos, provokes ERK activation outside of its injection site over a range of several cell diameters. The range and extent of ERK activation outside the source region is unchanged by co-injection of a dominant negative form of Ras, which blocks ERK-activation within the source. This suggests that FGF protein can diffuse over several cell diameters.
This article has been cited by other articles:
|
|
 |
|
  V. Picco, C. Hudson, and H. Yasuo Ephrin-Eph signalling drives the asymmetric division of notochord/neural precursors in Ciona embryos Development, April 15, 2007; 134(8): 1491 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schmerer, I. Torregroza, A. Pascal, M. Umbhauer, and T. Evans STAT5 acts as a repressor to regulate early embryonic erythropoiesis Blood, November 1, 2006; 108(9): 2989 - 2997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ebisuya, K. Kondoh, and E. Nishida The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity J. Cell Sci., July 15, 2005; 118(14): 2997 - 3002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Stern Neural induction: old problem, new findings, yet more questions Development, May 1, 2005; 132(9): 2007 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kuroda, L. Fuentealba, A. Ikeda, B. Reversade, and E.M. De Robertis Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation Genes & Dev., May 1, 2005; 19(9): 1022 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Bottcher and C. Niehrs Fibroblast Growth Factor Signaling during Early Vertebrate Development Endocr. Rev., February 1, 2005; 26(1): 63 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suzuki-Hirano, T. Sato, and H. Nakamura Regulation of isthmic Fgf8 signal by sprouty2 Development, January 15, 2005; 132(2): 257 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato and H. Nakamura The Fgf8 signal causes cerebellar differentiation by activating the Ras-ERK signaling pathway Development, September 1, 2004; 131(17): 4275 - 4285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Park, N. Warner, Y.-S. Bong, D. Stapleton, R. Maeda, T. Pawson, and I. O. Daar Ectopic EphA4 Receptor Induces Posterior Protrusions via FGF Signaling in Xenopus Embryos Mol. Biol. Cell, April 1, 2004; 15(4): 1647 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Murakami, S. A. Moody, I. O. Daar, and D. K. Morrison Morphogenesis during Xenopus gastrulation requires Wee1-mediated inhibition of cell proliferation Development, February 1, 2004; 131(3): 571 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lebreton, L. Boissel, and J. Moreau Control of embryonic Xenopus morphogenesis by a Ral-GDS/Xral branch of the Ras signalling pathway J. Cell Sci., November 15, 2003; 116(22): 4651 - 4662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Galli, A. Roure, R. Zeller, and R. Dono Glypican 4 modulates FGF signalling and regulates dorsoventral forebrain patterning in Xenopus embryos Development, October 15, 2003; 130(20): 4919 - 4929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Corson, Y. Yamanaka, K.-M. V. Lai, and J. Rossant Spatial and temporal patterns of ERK signaling during mouse embryogenesis Development, October 1, 2003; 130(19): 4527 - 4537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yokota, M. Kofron, M. Zuck, D. W. Houston, H. Isaacs, M. Asashima, C. C. Wylie, and J. Heasman A novel role for a nodal-related protein; Xnr3 regulates convergent extension movements via the FGF receptor Development, May 15, 2003; 130(10): 2199 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mood, R. Friesel, and I. O. Daar SNT1/FRS2 Mediates Germinal Vesicle Breakdown Induced by an Activated FGF Receptor1 in Xenopus Oocytes J. Biol. Chem., August 30, 2002; 277(36): 33196 - 33204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Park, N. Warner, K. Mood, T. Pawson, and I. O. Daar Low-Molecular-Weight Protein Tyrosine Phosphatase Is a Positive Component of the Fibroblast Growth Factor Receptor Signaling Pathway Mol. Cell. Biol., May 15, 2002; 22(10): 3404 - 3414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. David, L. Saint-Etienne, M. Tsang, T. F. Schilling, and F. M. Rosa Requirement for endoderm and FGF3 in ventral head skeleton formation Development, January 10, 2002; 129(19): 4457 - 4468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schohl and F. Fagotto {beta}-catenin, MAPK and Smad signaling during early Xenopus development Development, January 1, 2002; 129(1): 37 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. H. Li and A. L. Joyner Otx2 and Gbx2 are required for refinement and not induction of mid-hindbrain gene expression Development, December 15, 2001; 128(24): 4979 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sawada, M. Shinya, Y.-J. Jiang, A. Kawakami, A. Kuroiwa, and H. Takeda Fgf/MAPK signalling is a crucial positional cue in somite boundary formation Development, December 1, 2001; 128(23): 4873 - 4880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shinya, S. Koshida, A. Sawada, A. Kuroiwa, and H. Takeda Fgf signalling through MAPK cascade is required for development of the subpallial telencephalon in zebrafish embryos Development, November 1, 2001; 128(21): 4153 - 4164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Nutt, K. S. Dingwell, C. E. Holt, and E. Amaya Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not affect mesoderm induction and patterning Genes & Dev., May 1, 2001; 15(9): 1152 - 1166. [Abstract] [Full Text]
|
|
|
|
|
|
J. Lambert and L. Nagy MAPK signaling by the D quadrant embryonic organizer of the mollusc Ilyanassa obsoleta Development, January 1, 2001; 128(1): 45 - 56. [Abstract] [PDF]
|
|
|
|
|
|
M Umbhauer, A Penzo-Mendez, L Clavilier, J Boucaut, and J Riou Signaling specificities of fibroblast growth factor receptors in early Xenopus embryo J. Cell Sci., January 8, 2000; 113(16): 2865 - 2875. [Abstract] [PDF]
|
|
|
|
|
|
S Faure, M. Lee, T Keller, P ten Dijke, and M Whitman Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development Development, January 7, 2000; 127(13): 2917 - 2931. [Abstract] [PDF]
|
|
|
|
|
|
W Lerchner, B. Latinkic, J. Remacle, D Huylebroeck, and J. Smith Region-specific activation of the Xenopus brachyury promoter involves active repression in ectoderm and endoderm: a study using transgenic frog embryos Development, January 6, 2000; 127(12): 2729 - 2739. [Abstract] [PDF]
|
|
|
|
|
|
M Kofron, T Demel, J Xanthos, J Lohr, B Sun, H Sive, S Osada, C Wright, C Wylie, and J Heasman Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFbeta growth factors Development, January 12, 1999; 126(24): 5759 - 5770. [Abstract] [PDF]
|
|
|
|
|
|
A Rodaway, H Takeda, S Koshida, J Broadbent, B Price, J. Smith, R Patient, and N Holder Induction of the mesendoderm in the zebrafish germ ring by yolk cell-derived TGF-beta family signals and discrimination of mesoderm and endoderm by FGF Development, January 6, 1999; 126(14): 3067 - 3078. [Abstract] [PDF]
|
|
