|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 10 2149-2159, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
M Hild, A Dick, GJ Rauch, A Meier, T Bouwmeester, P Haffter and M Hammerschmidt
Hans-Spemann Laboratory, Max-Planck Institute of Immunobiology, Stuebeweg 51, D-79108 Freiburg, Germany. hammerschmid@immunbio.mpg. de.
Signaling by members of the TGFbeta superfamily is thought to be transduced by Smad proteins. Here, we describe a zebrafish mutant in smad5, designated somitabun (sbn). The dominant maternal and zygotic effect of the sbntc24 mutation is caused by a change in a single amino acid in the L3 loop of Smad5 protein which transforms Smad5 into an antimorphic version, inhibiting wild-type Smad5 and related Smad proteins. sbn mutant embryos are strongly dorsalized, similarly to mutants in Bmp2b, its putative upstream signal. Double mutant analyses and RNA injection experiments show that sbn and bmp2b interact and that sbn acts downstream of Bmp2b signaling to mediate Bmp2b autoregulation during early dorsoventral (D-V) pattern formation. Comparison of early marker gene expression patterns, chimera analyses and rescue experiments involving temporally controlled misexpression of bmp or smad in mutant embryos reveal three phases of D-V patterning: an early sbn- and bmp2b-independent phase when a coarse initial D-V pattern is set up, an intermediate sbn- and bmp2b-dependent phase during which the putative morphogenetic Bmp2/4 gradient is established, and a later sbn-independent phase during gastrulation when the Bmp2/4 gradient is interpreted and cell fates are specified.
This article has been cited by other articles:
|
|
 |
|
  L. J. McReynolds, S. Gupta, M. E. Figueroa, M. C. Mullins, and T. Evans Smad1 and Smad5 differentially regulate embryonic hematopoiesis Blood, December 1, 2007; 110(12): 3881 - 3890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gouttenoire, U. Valcourt, C. Bougault, E. Aubert-Foucher, E. Arnaud, L. Giraud, and F. Mallein-Gerin Knockdown of the Intraflagellar Transport Protein IFT46 Stimulates Selective Gene Expression in Mouse Chondrocytes and Affects Early Development in Zebrafish J. Biol. Chem., October 19, 2007; 282(42): 30960 - 30973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Carney, S. von der Hardt, C. Sonntag, A. Amsterdam, J. Topczewski, N. Hopkins, and M. Hammerschmidt Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermis Development, October 1, 2007; 134(19): 3461 - 3471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Reim and M. Brand Maternal control of vertebrate dorsoventral axis formation and epiboly by the POU domain protein Spg/Pou2/Oct4 Development, July 15, 2006; 133(14): 2757 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. J. Machingo, A. Fritz, and B. D. Shur A {beta}1,4-galactosyltransferase is required for Bmp2-dependent patterning of the dorsoventral axis during zebrafish embryogenesis Development, June 1, 2006; 133(11): 2233 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gupta, H. Zhu, L. I. Zon, and T. Evans BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis Development, June 1, 2006; 133(11): 2177 - 2187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rentzsch, J. Zhang, C. Kramer, W. Sebald, and M. Hammerschmidt Crossveinless 2 is an essential positive feedback regulator of Bmp signaling during zebrafish gastrulation Development, March 1, 2006; 133(5): 801 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Wilm and L. Solnica-Krezel Essential roles of a zebrafish prdm1/blimp1 homolog in embryo patterning and organogenesis Development, January 15, 2005; 132(2): 393 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Leung, J. Bischof, I. Soll, D. Niessing, D. Zhang, J. Ma, H. Jackle, and W. Driever bozozok directly represses bmp2b transcription and mediates the earliest dorsoventral asymmetry of bmp2b expression in zebrafish Development, August 15, 2003; 130(16): 3639 - 3649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yabe, T. Shimizu, O. Muraoka, Y.-K. Bae, T. Hirata, H. Nojima, A. Kawakami, T. Hirano, and M. Hibi Ogon/Secreted Frizzled functions as a negative feedback regulator of Bmp signaling Development, June 15, 2003; 130(12): 2705 - 2716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sidi, C. Goutel, N. Peyrieras, and F. M. Rosa Maternal induction of ventral fate by zebrafish radar PNAS, March 18, 2003; 100(6): 3315 - 3320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Roman, V. N. Pham, N. D. Lawson, M. Kulik, S. Childs, A. C. Lekven, D. M. Garrity, R. T. Moon, M. C. Fishman, R. J. Lechleider, et al. Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vessels Development, March 8, 2003; 129(12): 3009 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Thisse and L. I. Zon Organogenesis--Heart and Blood Formation from the Zebrafish Point of View Science, January 18, 2002; 295(5554): 457 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Imai, M. A. Gates, A. E. Melby, D. Kimelman, A. F. Schier, and W. S. Talbot The homeobox genes vox and vent are redundant repressors of dorsal fates in zebrafish Development, June 15, 2001; 128(12): 2407 - 2420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Bauer, Z Lele, G. Rauch, R Geisler, and M Hammerschmidt The type I serine/threonine kinase receptor Alk8/Lost-a-fin is required for Bmp2b/7 signal transduction during dorsoventral patterning of the zebrafish embryo Development, January 3, 2001; 128(6): 849 - 858. [Abstract] [PDF]
|
|
|
|
|
|
K. Mintzer, M. Lee, G Runke, J Trout, M Whitman, and M. Mullins Lost-a-fin encodes a type I BMP receptor, Alk8, acting maternally and zygotically in dorsoventral pattern formation Development, January 3, 2001; 128(6): 859 - 869. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Imai, B. Feldman, A. F. Schier, and W. S. Talbot Analysis of Chromosomal Rearrangements Induced by Postmeiotic Mutagenesis With Ethylnitrosourea in Zebrafish Genetics, May 1, 2000; 155(1): 261 - 272. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. S. Talbot and N. Hopkins Zebrafish mutations and functional analysis of the vertebrate genome Genes & Dev., April 1, 2000; 14(7): 755 - 762. [Full Text]
|
|
|
|
|
|
B Schmid, M Furthauer, S. Connors, J Trout, B Thisse, C Thisse, and M. Mullins Equivalent genetic roles for bmp7/snailhouse and bmp2b/swirl in dorsoventral pattern formation Development, January 3, 2000; 127(5): 957 - 967. [Abstract] [PDF]
|
|
|
|
|
|
V. Nguyen, J Trout, S. Connors, P Andermann, E Weinberg, and M. Mullins Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway Development, January 3, 2000; 127(6): 1209 - 1220. [Abstract] [PDF]
|
|
|
|
|
|
A Dick, M Hild, H Bauer, Y Imai, H Maifeld, A. Schier, W. Talbot, T Bouwmeester, and M Hammerschmidt Essential role of Bmp7 (snailhouse) and its prodomain in dorsoventral patterning of the zebrafish embryo Development, January 1, 2000; 127(2): 343 - 354. [Abstract] [PDF]
|
|
|
|
|
|
K. Barth, Y Kishimoto, K. Rohr, C Seydler, S Schulte-Merker, and S. Wilson Bmp activity establishes a gradient of positional information throughout the entire neural plate Development, January 11, 1999; 126(22): 4977 - 4987. [Abstract] [PDF]
|
|
|
|
|
|
A. Schuler-Metz, S. Knochel, E. Kaufmann, and W. Knochel The Homeodomain Transcription Factor Xvent-2 Mediates Autocatalytic Regulation of BMP-4 Expression in Xenopus Embryos J. Biol. Chem., October 27, 2000; 275(44): 34365 - 34374. [Abstract] [Full Text] [PDF]
|
|
