|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 11 2397-2407, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
KE Lewis, G Drossopoulou, IR Paton, DR Morrice, KE Robertson, DW Burt, PW Ingham and C Tickle
Developmental Genetics Programme, Krebs Institute, University of Sheffield, Sheffield, S10 2TN, UK.
talpid3 is an embryonic-lethal chicken mutation in a molecularly un-characterised autosomal gene. The recessive, pleiotropic phenotype includes polydactylous limbs with morphologically similar digits. Previous analysis established that hox-D and bmp genes, that are normally expressed posteriorly in the limb bud in response to a localised, posterior source of Sonic Hedgehog (Shh) are expressed symmetrically across the entire anteroposterior axis in talpid3 limb buds. In contrast, Shh expression itself is unaffected. Here we examine expression of patched (ptc), which encodes a component of the Shh receptor, and is probably itself a direct target of Shh signalling, to establish whether talpid3 acts in the Shh pathway. We find that ptc expression is significantly reduced in talpid3 embryos. We also demonstrate that talpid3 function is not required for Shh signal production but is required for normal response to Shh signals, implicating talpid3 in transduction of Shh signals in responding cells. Our analysis of expression of putative components of the Shh pathway, gli1, gli3 and coupTFII shows that genes regulated by Shh are either ectopically expressed or no longer responsive to Shh signals in talpid3 limbs, suggesting possible bifurcation in the Shh pathway. We also describe genetic mapping of gli1, ptc, shh and smoothened in chickens and confirm by co-segregation analysis that none of these genes correspond to talpid3.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Yin, F. Bangs, I. R. Paton, A. Prescott, J. James, M. G. Davey, P. Whitley, G. Genikhovich, U. Technau, D. W. Burt, et al. The Talpid3 gene (KIAA0586) encodes a centrosomal protein that is essential for primary cilia formation Development, February 15, 2009; 136(4): 655 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Burt Emergence of the Chicken as a Model Organism: Implications for Agriculture and Biology Poult. Sci., July 1, 2007; 86(7): 1460 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Huangfu and K. V. Anderson Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates Development, January 1, 2006; 133(1): 3 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Buttitta, R. Mo, C.-C. Hui, and C.-M. Fan Interplays of Gli2 and Gli3 and their requirement in mediating Shh-dependent sclerotome induction Development, December 22, 2003; 130(25): 6233 - 6243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Krebs, C. M. Schreiner, W. J. Scott Jr, S. M. Bell, D. J. Robbins, J. A. Goetz, H. Alt, N. Hawes, E. Wolf, and J. Favor Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud Development, December 15, 2003; 130(24): 6037 - 6047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Ahlgren, V. Thakur, and M. Bronner-Fraser Sonic hedgehog rescues cranial neural crest from cell death induced by ethanol exposure PNAS, August 6, 2002; 99(16): 10476 - 10481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Methot and K Basler An absolute requirement for Cubitus interruptus in Hedgehog signaling Development, January 3, 2001; 128(5): 733 - 742. [Abstract] [PDF]
|
|
|
|
|
|
M. Fernandez-Teran, M. E. Piedra, I. S. Kathiriya, D. Srivastava, J. C. Rodriguez-Rey, and M. A. Ros Role of dHAND in the anterior-posterior polarization of the limb bud: implications for the Sonic hedgehog pathway Development, May 15, 2000; 127(10): 2133 - 2142. [Abstract] [PDF]
|
|
|
|
|
|
A Gallet, C Angelats, S Kerridge, and P. Therond Cubitus interruptus-independent transduction of the Hedgehog signal in Drosophila Development, January 12, 2000; 127(24): 5509 - 5522. [Abstract] [PDF]
|
|
|
|
|
|
J. Sanz-Ezquerro and C Tickle Autoregulation of Shh expression and Shh induction of cell death suggest a mechanism for modulating polarising activity during chick limb development Development, January 11, 2000; 127(22): 4811 - 4823. [Abstract] [PDF]
|
|
|
|
|
|
G Drossopoulou, K. Lewis, J. Sanz-Ezquerro, N Nikbakht, A. McMahon, C Hofmann, and C Tickle A model for anteroposterior patterning of the vertebrate limb based on sequential long- and short-range Shh signalling and Bmp signalling Development, January 4, 2000; 127(7): 1337 - 1348. [Abstract] [PDF]
|
|
|
|
|
|
H. Park, C Bai, K. Platt, M. Matise, A Beeghly, C. Hui, M Nakashima, and A. Joyner Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation Development, January 4, 2000; 127(8): 1593 - 1605. [Abstract] [PDF]
|
|
|
|
|
|
A. Moon, A. Boulet, and M. Capecchi Normal limb development in conditional mutants of Fgf4 Development, January 3, 2000; 127(5): 989 - 996. [Abstract] [PDF]
|
|
