|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 11 2451-2460, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
M Andreazzoli, G Gestri, D Angeloni, E Menna and G Barsacchi
Laboratorio di Biologia Cellulare e dello Sviluppo, Universita' di Pisa, Via Carducci 13, Pisa, Italy.
The anteriormost part of the neural plate is fated to give rise to the retina and anterior brain regions. In Xenopus, this territory is initially included within the expression domain of the bicoid-class homeobox gene Xotx2 but very soon, at the beginning of neurulation, it becomes devoid of Xotx2 transcripts in spatiotemporal concomitance with the transcriptional activation of the paired-like homeobox gene Xrx1. By use of gain- and loss-of-function approaches, we have studied the role played by Xrx1 in the anterior neural plate and its interactions with other anterior homeobox genes. We find that, at early neurula stage Xrx1 is able to repress Xotx2 expression, thus first defining the retina-diencephalon territory in the anterior neural plate. Overexpression studies indicate that Xrx1 possesses a proliferative activity that is coupled with the specification of anterior fate. Expression of a Xrx1 dominant repressor construct (Xrx1-EnR) results in a severe impairment of eye and anterior brain development. Analysis of several brain markers in early Xrx1-EnR-injected embryos reveals that anterior deletions are preceded by a reduction of anterior gene expression domains in the neural plate. Accordingly, expression of anterior markers is abolished or decreased in animal caps coinjected with the neural inducer chordin and the Xrx1-EnR construct. The lack of expansion of mid-hindbrain markers, and the increase of apoptosis in the anterior neural plate after Xrx1-EnR injection, indicate that anterior deletions result from an early loss of anterior neural plate territories rather than posteriorization of the neuroectoderm. Altogether, these data suggest that Xrx1 plays a role in assigning anterior and proliferative properties to the rostralmost part of the neural plate, thus being required for eye and anterior brain development.
This article has been cited by other articles:
|
|
 |
|
  Y. Pan, S. Nekkalapudi, L. E. Kelly, and H. M. El-Hodiri The Rx-like Homeobox Gene (Rx-L) Is Necessary for Normal Photoreceptor Development. Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4245 - 4253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rembold, F. Loosli, R. J. Adams, and J. Wittbrodt Individual cell migration serves as the driving force for optic vesicle evagination. Science, August 25, 2006; 313(5790): 1130 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bidinost, M. Matsumoto, D. Chung, N. Salem, K. Zhang, D. W. Stockton, A. Khoury, A. Megarbane, B. A. Bejjani, and E. I. Traboulsi Heterozygous and Homozygous Mutations in PITX3 in a Large Lebanese Family with Posterior Polar Cataracts and Neurodevelopmental Abnormalities. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1274 - 1280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gestri, M. Carl, I. Appolloni, S. W. Wilson, G. Barsacchi, and M. Andreazzoli Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting Bmp4 expression Development, May 15, 2005; 132(10): 2401 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lupo, Y. Liu, R. Qiu, R. A. S. Chandraratna, G. Barsacchi, R.-Q. He, and W. A. Harris Dorsoventral patterning of the Xenopus eye: a collaboration of Retinoid, Hedgehog and FGF receptor signaling Development, April 1, 2005; 132(7): 1737 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tabata, Y. Ouchi, H. Kamiya, T. Manabe, K.-i. Arai, and S. Watanabe Specification of the Retinal Fate of Mouse Embryonic Stem Cells by Ectopic Expression of Rx/rax, a Homeobox Gene Mol. Cell. Biol., May 15, 2004; 24(10): 4513 - 4521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Andreazzoli, G. Gestri, F. Cremisi, S. Casarosa, I. B. Dawid, and G. Barsacchi Xrx1 controls proliferation and neurogenesis in Xenopus anterior neural plate Development, November 1, 2003; 130(21): 5143 - 5155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Zuber, G. Gestri, A. S. Viczian, G. Barsacchi, and W. A. Harris Specification of the vertebrate eye by a network of eye field transcription factors Development, November 1, 2003; 130(21): 5155 - 5167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. O. Hartley, S. L. Nutt, and E. Amaya Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system PNAS, January 24, 2002; (2002) 22646899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-M. A. Chen and C. L. Cepko The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation Development, January 12, 2002; 129(23): 5363 - 5375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lupo, W. A. Harris, G. Barsacchi, and R. Vignali Induction and patterning of the telencephalon in Xenopus laevis Development, January 12, 2002; 129(23): 5421 - 5436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Loosli, S. Winkler, C. Burgtorf, E. Wurmbach, W. Ansorge, T. Henrich, C. Grabher, D. Arendt, M. Carl, A. Krone, et al. Medaka eyeless is the key factor linking retinal determination and eye growth Development, October 15, 2001; 128(20): 4035 - 4044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Withington, R Beddington, and J Cooke Foregut endoderm is required at head process stages for anteriormost neural patterning in chick Development, January 2, 2001; 128(3): 309 - 320. [Abstract] [PDF]
|
|
|
|
|
|
M Schwarz, F Cecconi, G Bernier, N Andrejewski, B Kammandel, M Wagner, and P Gruss Spatial specification of mammalian eye territories by reciprocal transcriptional repression of Pax2 and Pax6 Development, January 10, 2000; 127(20): 4325 - 4334. [Abstract] [PDF]
|
|
|
|
|
|
Z Hardcastle and N Papalopulu Distinct effects of XBF-1 in regulating the cell cycle inhibitor p27(XIC1) and imparting a neural fate Development, January 3, 2000; 127(6): 1303 - 1314. [Abstract] [PDF]
|
|
|
|
|
|
R. Chow, C. Altmann, R. Lang, and A Hemmati-Brivanlou Pax6 induces ectopic eyes in a vertebrate Development, January 10, 1999; 126(19): 4213 - 4222. [Abstract] [PDF]
|
|
|
|
|
|
K. O. Hartley, S. L. Nutt, and E. Amaya Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system PNAS, February 5, 2002; 99(3): 1377 - 1382. [Abstract] [Full Text] [PDF]
|
|
