|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 12 2759-2770, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
P Wai, B Truong and KM Bhat
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Cell intrinsic and cell extrinsic factors mediate asymmetric cell divisions during neurogenesis in the Drosophila embryo. In the NB4-2->GMC-1->RP2/sib lineage, one of the well-studied neuronal lineages in the ventral nerve cord, the Notch (N) signaling interacts with the asymmetrically localized Numb (Nb) to specify sibling neuronal fates to daughter cells of GMC-1. In this current study, we have investigated asymmetric cell fate specifications by N and Nb in the context of cell cycle. We have used loss-of-function mutations in N and nb, cell division mutants cyclinA (cycA), regulator of cyclin A1 (rca1) and string/cdc25 phosphatase (stg), and the microtubule destabilizing agent, nocodazole, to investigate this issue. We report that the loss of cycA, rca1 or stg leads to a block in the division of GMC-1, however, this GMC-1 exclusively adopts an RP2 identity. While the loss of N leads to the specification of RP2 fates to both progeny of GMC-1 and loss of nb results in the specification of sib fates to these daughter cells, the GMC-1 in the double mutant between nb and cycA assumes a sib fate. These epistasis results indicate that both N and nb function downstream of cell division genes and that progression through cell cycle is required for the asymmetric localization of Nb. In the absence of entry to metaphase, the Nb protein prevents the N signaling from specifying sib fate to the RP2/sib precursor. These results are also consistent with our finding that the sib cell is specified as RP2 in N; nb double mutants. Finally, our results show that nocodazole-arrested GMC-1 in wild-type embryos randomly assumes either an RP2 fate or a sib fate. This suggests that microtubules are involved in mediating the antagonistic interaction between Nb and N during RP2 and sib fate specification.
This article has been cited by other articles:
|
|
 |
|
  A. El Wakil, C. Francius, A. Wolff, J. Pleau-Varet, and J. Nardelli The GATA2 transcription factor negatively regulates the proliferation of neuronal progenitors Development, June 1, 2006; 133(11): 2155 - 2165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Bhat and N. Apsel Upregulation of Mitimere and Nubbin acts through Cyclin E to confer self-renewing asymmetric division potential to neural precursor cells Development, March 1, 2004; 131(5): 1123 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yedvobnick, A. Kumar, P. Chaudhury, J. Opraseuth, N. Mortimer, and K. M. Bhat Differential Effects of Drosophila Mastermind on Asymmetric Cell Fate Specification and Neuroblast Formation Genetics, March 1, 2004; 166(3): 1281 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Han and R. Bodmer Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division Development, July 1, 2003; 130(13): 3039 - 3051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Novotny, R. Eiselt, and J. Urban Hunchback is required for the specification of the early sublineage of neuroblast 7-3 in the Drosophila central nervous system Development, March 4, 2003; 129(4): 1027 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mehta and K. M. Bhat Slit signaling promotes the terminal asymmetric division of neural precursor cells in the Drosophila CNS Development, August 15, 2001; 128(16): 3161 - 3168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Dang, A. F. Gazdar, A. K. Virmani, T. Sepetavec, K. R. Hande, J. D. Minna, J. R. Roberts, and D. P. Carbone Chromosome 19 Translocation, Overexpression of Notch3, and Human Lung Cancer J Natl Cancer Inst, August 16, 2000; 92(16): 1355 - 1357. [Full Text] [PDF]
|
|
|
|
|
|
D. Fay and M Han Mutations in cye-1, a Caenorhabditis elegans cyclin E homolog, reveal coordination between cell-cycle control and vulval development Development, January 9, 2000; 127(18): 4049 - 4060. [Abstract] [PDF]
|
|
|
|
|
|
P. Cheah, W Chia, and X Yang Jumeaux, a novel Drosophila winged-helix family protein, is required for generating asymmetric sibling neuronal cell fates Development, January 8, 2000; 127(15): 3325 - 3335. [Abstract] [PDF]
|
|
|
|
|
|
K. Bhat, E. van Beers, and P Bhat Sloppy paired acts as the downstream target of wingless in the Drosophila CNS and interaction between sloppy paired and gooseberry inhibits sloppy paired during neurogenesis Development, January 2, 2000; 127(3): 655 - 665. [Abstract] [PDF]
|
|
