|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 22 4943-4953, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
AP Chan, M Kloc and LD Etkin
Department of Molecular Genetics, University of Texas, MD Anderson Cancer Center, Houston TX 77030, USA.
Vegetally localized transcripts have been implicated in a number of important biological functions, including cell fate determination and embryonic patterning. We have isolated a cDNA, fatvg, which encodes a localized maternal transcript that exhibits a localization pattern reminiscent of Vg1 mRNA. fatvg is the homologue of a mammalian gene expressed in adipose tissues. The fatvg transcript, unlike Vg1 which localizes strictly through the Late pathway, also associates with the mitochondrial cloud that is characteristic of the METRO or Early pathway. This suggests that fatvg mRNA may utilize both the METRO and Late pathways to localize to the vegetal cortex during oogenesis. We have dissected the cis-acting localization elements of fatvg mRNA and compared these elements with Vg1 mRNA. Our results indicate that, like most localized RNAs, in a variety of systems, transcripts of fatvg contain localization elements in the 3'UTR. The 3'UTR of fatvg mRNA contains multiple elements that are able to function independently; however, it functions most efficiently when all of the elements are present. We have defined a short 25-nucleotide element that can direct vegetal localization as a single copy. This element differs in sequence from previously described Vg1 localization elements, suggesting that different localization elements are involved in the localization of RNAs through the Late pathway.
This article has been cited by other articles:
|
|
 |
|
  R. A. Jain and E. R. Gavis The Drosophila hnRNP M homolog Rumpelstiltskin regulates nanos mRNA localization Development, March 1, 2008; 135(5): 973 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Jambhekar and J. L. DeRisi Cis-acting determinants of asymmetric, cytoplasmic RNA transport RNA, May 1, 2007; 13(5): 625 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Scofield, X. Hong, and M. Lynch Position of the Final Intron in Full-Length Transcripts: Determined by NMD? Mol. Biol. Evol., April 1, 2007; 24(4): 896 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kloc and L. D. Etkin RNA localization mechanisms in oocytes J. Cell Sci., January 15, 2005; 118(2): 269 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chang, J. Torres, R. A. Lewis, K. L. Mowry, E. Houliston, and M. L. King Localization of RNAs to the Mitochondrial Cloud in Xenopus Oocytes through Entrapment and Association with Endoplasmic Reticulum Mol. Biol. Cell, October 1, 2004; 15(10): 4669 - 4681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Claussen, K. Horvay, and T. Pieler Evidence for overlapping, but not identical, protein machineries operating in vegetal RNA localization along early and late pathways in Xenopus oocytes Development, September 1, 2004; 131(17): 4263 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hamada, K. Ishiyama, C. Sakulsingharoj, S.-B. Choi, Y. Wu, C. Wang, S. Singh, N. Kawai, J. Messing, and T. W. Okita Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm PLANT CELL, October 1, 2003; 15(10): 2265 - 2272. [Abstract] [Full Text] [PDF]
|
|
