|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 22 4965-4976, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
AJ Bendall, J Ding, G Hu, MM Shen and C Abate-Shen
Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology and Department of Pediatrics, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
The migration of myogenic precursors to the vertebrate limb exemplifies a common problem in development - namely, how migratory cells that are committed to a specific lineage postpone terminal differentiation until they reach their destination. Here we show that in chicken embryos, expression of the Msx1 homeobox gene overlaps with Pax3 in migrating limb muscle precursors, which are committed myoblasts that do not express myogenic differentiation genes such as MyoD. We find that ectopic expression of Msx1 in the forelimb and somites of chicken embryos inhibits MyoD expression as well as muscle differentiation. Conversely, ectopic expression of Pax3 activates MyoD expression, while co-ectopic expression of Msx1 and Pax3 neutralizes their effects on MyoD. Moreover, we find that Msx1 represses and Pax3 activates MyoD regulatory elements in cell culture, while in combination, Msx1 and Pax3 oppose each other's trancriptional actions on MyoD. Finally, we show that the Msx1 protein interacts with Pax3 in vitro, thereby inhibiting DNA binding by Pax3. Thus, we propose that Msx1 antagonizes the myogenic activity of Pax3 in migrating limb muscle precursors via direct protein-protein interaction. Our results implicate functional antagonism through competitive protein-protein interactions as a mechanism for regulating the differentiation state of migrating cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Takahashi, A. Kamiya, A. Ishiguro, A. C. Suzuki, N. Saitou, A. Toyoda, and J. Aruga Conservation and Diversification of Msx Protein in Metazoan Evolution Mol. Biol. Evol., January 1, 2008; 25(1): 69 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hayashi, S. Nakamura, W. Nishida, and K. Sobue Bone Morphogenetic Protein-Induced Msx1 and Msx2 Inhibit Myocardin-Dependent Smooth Muscle Gene Transcription Mol. Cell. Biol., December 15, 2006; 26(24): 9456 - 9470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Diamond, M. Amen, Q. Hu, H. M. Espinoza, and B. A. Amendt Functional interactions between Dlx2 and lymphoid enhancer factor regulate Msx2 Nucleic Acids Res., November 6, 2006; 34(20): 5951 - 5965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Shi and D. J. Garry Muscle stem cells in development, regeneration, and disease. Genes & Dev., July 1, 2006; 20(13): 1692 - 1708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lee, J. C. Quinn, K. V. Prasanth, V. A. Swiss, K. D. Economides, M. M. Camacho, D. L. Spector, and C. Abate-Shen PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein. Genes & Dev., April 1, 2006; 20(7): 784 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Kim, J.P. Simmer, B.P.-J. Lin, and J.C.-C. Hu Novel MSX1 Frameshift Causes Autosomal-dominant Oligodontia Journal of Dental Research, March 1, 2006; 85(3): 267 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Givens, N. Rave-Harel, V. D. Goonewardena, R. Kurotani, S. E. Berdy, C. H. Swan, J. L. R. Rubenstein, B. Robert, and P. L. Mellon Developmental Regulation of Gonadotropin-releasing Hormone Gene Expression by the MSX and DLX Homeodomain Protein Families J. Biol. Chem., May 13, 2005; 280(19): 19156 - 19165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-A. Bruun, E. I. S. Thomassen, K. Kristiansen, G. Tylden, T. Holm, I. Mikkola, G. Bjorkoy, and T. Johansen The third helix of the homeodomain of paired class homeodomain proteins acts as a recognition helix both for DNA and protein interactions Nucleic Acids Res., May 10, 2005; 33(8): 2661 - 2675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Apuzzo, A. Abdelhakim, A. S. Fortin, and P. Gros Cross-talk between the Paired Domain and the Homeodomain of Pax3: DNA BINDING BY EACH DOMAIN CAUSES A STRUCTURAL CHANGE IN THE OTHER DOMAIN, SUPPORTING INTERDEPENDENCE FOR DNA BINDING J. Biol. Chem., August 6, 2004; 279(32): 33601 - 33612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lee, R. Habas, and C. Abate-Shen Msx1 Cooperates with Histone H1b for Inhibition of Transcription and Myogenesis Science, June 11, 2004; 304(5677): 1675 - 1678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, A. W. Helms, and J. E. Johnson Distinct activities of Msx1 and Msx3 in dorsal neural tube development Development, March 1, 2004; 131(5): 1017 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. P. CHARGE and M. A. RUDNICKI Cellular and Molecular Regulation of Muscle Regeneration Physiol Rev, January 1, 2004; 84(1): 209 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Timmer, C. Wang, and L. Niswander BMP signaling patterns the dorsal and intermediate neural tube via regulation of homeobox and helix-loop-helix transcription factors Development, March 7, 2003; 129(10): 2459 - 2472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kwang, S. M. Brugger, A. Lazik, A. E. Merrill, L.-Y. Wu, Y.-H. Liu, M. Ishii, F. O. Sangiorgi, M. Rauchman, H. M. Sucov, et al. Msx2 is an immediate downstream effector of Pax3 in the development of the murine cardiac neural crest Development, March 3, 2003; 129(2): 527 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Meech, H. Makarenkova, D. B. Edelman, and F. S. Jones The Homeodomain Protein Barx2 Promotes Myogenic Differentiation and Is Regulated by Myogenic Regulatory Factors J. Biol. Chem., February 28, 2003; 278(10): 8269 - 8278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Benassayag, S. Plaza, P. Callaerts, J. Clements, Y. Romeo, W. J. Gehring, and D. L. Cribbs Evidence for a direct functional antagonism of the selector genes proboscipedia and eyeless in Drosophila head development Development, February 1, 2003; 130(3): 575 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. de Santa Barbara and D. J. Roberts Tail gut endoderm and gut/genitourinary/tail development: a new tissue-specific role for Hoxa13 Development, January 2, 2002; 129(3): 551 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Hawke and D. J. Garry Myogenic satellite cells: physiology to molecular biology J Appl Physiol, August 1, 2001; 91(2): 534 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hu, H. Lee, S. M. Price, M. M. Shen, and C. Abate-Shen Msx homeobox genes inhibit differentiation through upregulation of cyclin D1 Development, June 15, 2001; 128(12): 2373 - 2384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Fredericks, K. Ayyanathan, M. Herlyn, J. R. Friedman, and F. J. Rauscher III An Engineered PAX3-KRAB Transcriptional Repressor Inhibits the Malignant Phenotype of Alveolar Rhabdomyosarcoma Cells Harboring the Endogenous PAX3-FKHR Oncogene Mol. Cell. Biol., July 15, 2000; 20(14): 5019 - 5031. [Abstract] [Full Text]
|
|
|
|
|
|
D. Houzelstein, Y. Cheraud, G. Auda-Boucher, J. Fontaine-Perus, and B. Robert The expression of the homeobox gene Msx1 reveals two populations of dermal progenitor cells originating from the somites Development, May 15, 2000; 127(10): 2155 - 2164. [Abstract] [PDF]
|
|
|
|
|
|
M Delfini, E Hirsinger, O Pourquie, and D Duprez Delta 1-activated notch inhibits muscle differentiation without affecting Myf5 and Pax3 expression in chick limb myogenesis Development, January 12, 2000; 127(23): 5213 - 5224. [Abstract] [PDF]
|
|
|
|
|
|
F. Relaix and M. Buckingham From insect eye to vertebrate muscle: redeployment of a regulatory network Genes & Dev., December 15, 1999; 13(24): 3171 - 3178. [Full Text]
|
|
|
|
|
|
C. S. K. Mayanil, D. George, L. Freilich, E. J. Miljan, B. Mania-Farnell, D. G. McLone, and E. G. Bremer Microarray Analysis Detects Novel Pax3 Downstream Target Genes J. Biol. Chem., December 21, 2001; 276(52): 49299 - 49309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Blin-Wakkach, F. Lezot, S. Ghoul-Mazgar, D. Hotton, S. Monteiro, C. Teillaud, L. Pibouin, S. Orestes-Cardoso, P. Papagerakis, M. Macdougall, et al. Endogenous Msx1 antisense transcript: In vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals PNAS, June 19, 2001; 98(13): 7336 - 7341. [Abstract] [Full Text] [PDF]
|
|
