|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 24 5581-5589, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
X Li, A Veraksa and W McGinnis
Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Hox transcription factors, in combination with cofactors such as PBC proteins, provide diverse developmental fates to cells on the anteroposterior body axis of animal embryos. However, the mechanisms by which the different Hox proteins and their cofactors generate those diverse fates remain unclear. Recent findings have provided support for a model where the DNA binding sites that directly interact with Hox-PBC heterodimers determine which member of the Hox protein family occupies and thereby regulates a given target element. In the experiments reported here, we test the function of chimeric Hox response elements and, surprisingly, find evidence that runs counter to this view. A 21 bp cofactor binding sequence from an embryonic Deformed Hox response element, containing no Hox or Hox-PBC binding sites, was combined with single or multimeric sites that bind heterodimers of Labial-type Hox and PBC proteins. Normally, multimerized Labial-PBC binding sites are sufficient to trigger a Labial-specific activation response in either Drosophila or mouse embryos. Here we find that the 21 bp sequence element plays an important role in Deformed specificity, as it is capable of switching a Labial-PBC binding site/response element to a Deformed response element. Thus, cofactor binding sites that are separate and distinct from homeodomain binding sites can dictate the regulatory specificity of a Hox response element.
This article has been cited by other articles:
|
|
 |
|
  D. E. Reed, X. M. Huang, J. A. Wohlschlegel, M. S. Levine, and K. Senger DEAF-1 regulates immunity gene expression in Drosophila PNAS, June 17, 2008; 105(24): 8351 - 8356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bondos, X.-X. Tan, and K. S. Matthews Physical and Genetic Interactions Link Hox Function with Diverse Transcription Factors and Cell Signaling Proteins Mol. Cell. Proteomics, May 1, 2006; 5(5): 824 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. McCabe and J. W. Innis A genomic approach to the identification and characterization of HOXA13 functional binding elements Nucleic Acids Res., November 30, 2005; 33(21): 6782 - 6794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ferretti, F. Cambronero, S. Tumpel, E. Longobardi, L. M. Wiedemann, F. Blasi, and R. Krumlauf Hoxb1 Enhancer and Control of Rhombomere 4 Expression: Complex Interplay between PREP1-PBX1-HOXB1 Binding Sites Mol. Cell. Biol., October 1, 2005; 25(19): 8541 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Hersh and S. B. Carroll Direct regulation of knot gene expression by Ultrabithorax and the evolution of cis-regulatory elements in Drosophila Development, April 1, 2005; 132(7): 1567 - 1577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ebner, C. Cabernard, M. Affolter, and S. Merabet Recognition of distinct target sites by a unique Labial/Extradenticle/Homothorax complex Development, April 1, 2005; 132(7): 1591 - 1600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Merabet, F. Catala, J. Pradel, and Y. Graba A Green Fluorescent Protein Reporter Genetic Screen That Identifies Modifiers of Hox Gene Function in the Drosophila Embryo Genetics, September 1, 2002; 162(1): 189 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-F. Shen, K. Krishnan, H. J. Lawrence, and C. Largman The HOX Homeodomain Proteins Block CBP Histone Acetyltransferase Activity Mol. Cell. Biol., November 1, 2001; 21(21): 7509 - 7522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Manzanares, S. Bel-Vialar, L. Ariza-McNaughton, E. Ferretti, H. Marshall, M. M. Maconochie, F. Blasi, and R. Krumlauf Independent regulation of initiation and maintenance phases of Hoxa3 expression in the vertebrate hindbrain involve auto- and cross-regulatory mechanisms Development, September 15, 2001; 128(18): 3595 - 3607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Capovilla, Z Kambris, and J Botas Direct regulation of the muscle-identity gene apterous by a Hox protein in the somatic mesoderm Development, January 4, 2001; 128(8): 1221 - 1230. [Abstract] [PDF]
|
|
|
|
|
|
W. Geng, B. He, M. Wang, and P. N. Adler The tricornered Gene, Which Is Required for the Integrity of Epidermal Cell Extensions, Encodes the Drosophila Nuclear DBF2-Related Kinase Genetics, December 1, 2000; 156(4): 1817 - 1828. [Abstract] [Full Text]
|
|
|
|
|
|
C. Zhao, V. Dave, F. Yang, T. Scarborough, and J. Ma Target Selectivity of Bicoid Is Dependent on Nonconsensus Site Recognition and Protein-Protein Interaction Mol. Cell. Biol., November 1, 2000; 20(21): 8112 - 8123. [Abstract] [Full Text]
|
|
|
|
|
|
A Veraksa, N McGinnis, X Li, J Mohler, and W McGinnis Cap 'n' collar B cooperates with a small Maf subunit to specify pharyngeal development and suppress deformed homeotic function in the Drosophila head Development, January 9, 2000; 127(18): 4023 - 4037. [Abstract] [PDF]
|
|
|
|
|
|
A Nasiadka, A Grill, and H. Krause Mechanisms regulating target gene selection by the homeodomain-containing protein Fushi tarazu Development, January 7, 2000; 127(13): 2965 - 2976. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Pan, Y. Xie, T. A. Black, C. A. Jones, S. C. Pruitt, and K. W. Gross An Abd-B Class HOX{middle dot}PBX Recognition Sequence Is Required for Expression from the Mouse Ren-1c Gene J. Biol. Chem., August 24, 2001; 276(35): 32489 - 32494. [Abstract] [Full Text] [PDF]
|
|
