|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 24 5621-5634, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
M Watanabe and M Whitman
Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USA.
We have examined the role of the maternally encoded transcription factor FAST-1 in the establishment of the mesodermal transcriptional program in Xenopus embryos. FAST-1 has been shown to associate with Smad2 and Smad4, transducers of TGFbeta superfamily signals, in response to stimulation by several TGFbeta superfamily ligands. The FAST-1/Smad2/Smad4 complex binds and activates a 50 bp activin responsive element identified in the promoter of the meso-endodermal marker Mix.2. We have now used three complementary approaches to demonstrate that FAST-1 is a central regulator of mesoderm induction by ectopic TGFbeta superfamily ligands and during endogenous patterning: ectopic expression of mutationally activated FAST-1, ectopic expression of dominant inhibitory FAST-1, and injection of a blocking antibody specific for FAST-1. Expression of constitutively transcriptionally active FAST-1 fusion protein (FAST-VP16(A)) in prospective ectoderm can directly induce the same set of general and dorsal mesodermal genes, as well as some endodermal genes, as are induced by activin or Vg1. In intact embryos, this construct can induce secondary axes similar to those induced by activin or Vg1. Conversely, expression of a FAST-1-repressor fusion (FAST-En(R)) in prospective ectoderm blocks induction of mesodermal genes by activin, while expression of FAST-En(R) in intact embryos prevents general/dorsal mesodermal gene expression and axial development. Injection of a blocking antibody specific for FAST-1 prevents induction of mesodermal response genes by activin or Vg1, but not by FGF. In intact embryos, this antibody can prevent the expression of early mesodermal markers and inhibit axis formation, demonstrating that FAST-1 is a necessary component of the first steps in the specification of mesoderm.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Ueno, N. Nakajo, M. Watanabe, M. Isoda, and N. Sagata FoxM1-driven cell division is required for neuronal differentiation in early Xenopus embryos Development, June 1, 2008; 135(11): 2023 - 2030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Shen Nodal signaling: developmental roles and regulation Development, March 15, 2007; 134(6): 1023 - 1034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Yun, S.-C. Choi, E. Park, S.-J. Kim, A.-S. Chung, H.-K. Lee, H.-J. Lee, and J.-K. Han Negative regulation of Activin/Nodal signaling by SRF during Xenopus gastrulation Development, February 15, 2007; 134(4): 769 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yaklichkin, A. B. Steiner, Q. Lu, and D. S. Kessler FoxD3 and Grg4 Physically Interact to Repress Transcription and Induce Mesoderm in Xenopus J. Biol. Chem., January 26, 2007; 282(4): 2548 - 2557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chen, S. M. Ware, A. Sato, D. E. Houston-Hawkins, R. Habas, M. M. Matzuk, M. M. Shen, and C. W. Brown The Vg1-related protein Gdf3 acts in a Nodal signaling pathway in the pre-gastrulation mouse embryo Development, January 15, 2006; 133(2): 319 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Onuma, C.-Y. Yeo, and M. Whitman XCR2, one of three Xenopus EGF-CFC genes, has a distinct role in the regulation of left-right patterning Development, January 15, 2006; 133(2): 237 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ding, Q. Tang, V. Espina, L. A. Liotta, D. T. Mauger, and K. M. Mulder A Transforming Growth Factor-{beta} Receptor-Interacting Protein Frequently Mutated in Human Ovarian Cancer Cancer Res., August 1, 2005; 65(15): 6526 - 6533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Koide, T. Hayata, and K. W. Y. Cho Gene Regulatory Networks Special Feature: Xenopus as a model system to study transcriptional regulatory networks PNAS, April 5, 2005; 102(14): 4943 - 4948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Afouda, A. Ciau-Uitz, and R. Patient GATA4, 5 and 6 mediate TGF{beta} maintenance of endodermal gene expression in Xenopus embryos Development, February 15, 2005; 132(4): 763 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Daniels, K. Shimizu, A. M. Zorn, and S.-i. Ohnuma Negative regulation of Smad2 by PIASy is required for proper Xenopus mesoderm formation Development, November 15, 2004; 131(22): 5613 - 5626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kofron, H. Puck, H. Standley, C. Wylie, R. Old, M. Whitman, and J. Heasman New roles for FoxH1 in patterning the early embryo Development, October 15, 2004; 131(20): 5065 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Morkel, J. Huelsken, M. Wakamiya, J. Ding, M. van de Wetering, H. Clevers, M. M. Taketo, R. R. Behringer, M. M. Shen, and W. Birchmeier {beta}-Catenin regulates Cripto- and Wnt3-dependent gene expression programs in mouse axis and mesoderm formation Development, December 22, 2003; 130(25): 6283 - 6294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Kunwar, S. Zimmerman, J. T. Bennett, Y. Chen, M. Whitman, and A. F. Schier Mixer/Bon and FoxH1/Sur have overlapping and divergent roles in Nodal signaling and mesendoderm induction Development, December 1, 2003; 130(23): 5589 - 5599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takebayashi-Suzuki, J. Funami, D. Tokumori, A. Saito, T. Watabe, K. Miyazono, A. Kanda, and A. Suzuki Interplay between the tumor suppressor p53 and TGF{beta} signaling shapes embryonic body axes in Xenopus Development, September 1, 2003; 130(17): 3929 - 3939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hiratani, N. Yamamoto, T. Mochizuki, S.-y. Ohmori, and M. Taira Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1 expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer functions Development, September 1, 2003; 130(17): 4161 - 4175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Howell, G. J. Inman, and C. S. Hill A novel Xenopus Smad-interacting forkhead transcription factor (XFast-3) cooperates with XFast-1 in regulating gastrulation movements Development, March 8, 2003; 129(12): 2823 - 2834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guerrero-Esteo, T. Sanchez-Elsner, A. Letamendia, and C. Bernabeu Extracellular and Cytoplasmic Domains of Endoglin Interact with the Transforming Growth Factor-beta Receptors I and II J. Biol. Chem., August 2, 2002; 277(32): 29197 - 29209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T. Yan, J.-J. Liu, Y. Luo, C. E, R. S. Haltiwanger, C. Abate-Shen, and M. M. Shen Dual Roles of Cripto as a Ligand and Coreceptor in the Nodal Signaling Pathway Mol. Cell. Biol., July 1, 2002; 22(13): 4439 - 4449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Y.R. Stainier A glimpse into the molecular entrails of endoderm formation Genes & Dev., April 15, 2002; 16(8): 893 - 907. [Full Text] [PDF]
|
|
|
|
|
|
C. Ring, S. Ogata, L. Meek, J. Song, T. Ohta, K. Miyazono, and K. W.Y. Cho The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-containing transcription factor in activin/nodal signaling Genes & Dev., April 1, 2002; 16(7): 820 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Smith, S. I. Rayter, C. Niederlander, J. Spicer, C. M. Jones, and A. Ashworth LIP1, a cytoplasmic protein functionally linked to the Peutz-Jeghers syndrome kinase LKB1 Hum. Mol. Genet., December 1, 2001; 10(25): 2869 - 2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Yamamoto, C. Takagi, A. C. Hyodo, and N. Ueno Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling Development, July 15, 2001; 128(14): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shimizu, P.-Y. Bourillot, S. J. Nielsen, A. M. Zorn, and J. B. Gurdon Swift Is a Novel BRCT Domain Coactivator of Smad2 in Transforming Growth Factor {beta} Signaling Mol. Cell. Biol., June 15, 2001; 21(12): 3901 - 3912. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. P. v. Strandmann, S. Senkel, G. Ryffel, and U. R. Hengge Dimerization Co-Factor of Hepatocyte Nuclear Factor 1/Pterin-4{{alpha}}-Carbinolamine Dehydratase Is Necessary for Pigmentation in Xenopus and Overexpressed in Primary Human Melanoma Lesions Am. J. Pathol., June 1, 2001; 158(6): 2021 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, C. Meno, Y. Sakai, H. Shiratori, K. Mochida, Y. Ikawa, Y. Saijoh, and H. Hamada The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse Genes & Dev., May 15, 2001; 15(10): 1242 - 1256. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. Hoodless, M. Pye, C. Chazaud, E. Labbé, L. Attisano, J. Rossant, and J. L. Wrana FoxH1 (Fast) functions to specify the anterior primitive streak in the mouse Genes & Dev., May 15, 2001; 15(10): 1257 - 1271. [Abstract] [Full Text]
|
|
|
|
|
|
F Muller, S Albert, P Blader, N Fischer, M Hallonet, and U Strahle Direct action of the nodal-related signal cyclops in induction of sonic hedgehog in the ventral midline of the CNS Development, January 9, 2000; 127(18): 3889 - 3897. [Abstract] [PDF]
|
|
|
|
|
|
S Faure, M. Lee, T Keller, P ten Dijke, and M Whitman Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development Development, January 7, 2000; 127(13): 2917 - 2931. [Abstract] [PDF]
|
|
|
|
|
|
S. Osada, Y Saijoh, A Frisch, C. Yeo, H Adachi, M Watanabe, M Whitman, H Hamada, and C. Wright Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1 Development, January 6, 2000; 127(11): 2503 - 2514. [Abstract] [PDF]
|
|
|
|
|
|
A. Scherer and J. M. Graff Calmodulin Differentially Modulates Smad1 and Smad2 Signaling J. Biol. Chem., December 22, 2000; 275(52): 41430 - 41438. [Abstract] [Full Text] [PDF]
|
|
