|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 24 5833-5846, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
KJ Beumer, J Rohrbough, A Prokop and K Broadie
Biology Department, University of Utah, Salt Lake City, UT 84112, USA.
A family of three position-specific (PS) integrins are expressed at the Drosophila neuromuscular junction (NMJ): a beta subunit ((betaPS), expressed in both presynaptic and postsynaptic membranes, and two alpha subunits (alphaPS1, alphaPS2), expressed at least in the postsynaptic membrane. PS integrins appear at postembryonic NMJs coincident with the onset of rapid morphological growth and terminal type-specific differentiation, and are restricted to type I synaptic boutons, which mediate fast, excitatory glutamatergic transmission. We show that two distinctive hypomorphic mutant alleles of the beta subunit gene myospheroid (mys(b9) and mys(ts1)), differentially affect betaPS protein expression at the synapse to produce distinctive alterations in NMJ branching, bouton formation, synaptic architecture and the specificity of synapse formation on target cells. The mys(b9) mutation alters betaPS localization to cause a striking reduction in NMJ branching, bouton size/number and the formation of aberrant 'mini-boutons', which may represent a developmentally arrested state. The mys(ts1) mutation strongly reduces betaPS expression to cause the opposite phenotype of excessive synaptic sprouting and morphological growth. NMJ function in these mutant conditions is altered in line with the severity of the morphological aberrations. Consistent with these mutant phenotypes, transgenic overexpression of the betaPS protein with a heat-shock construct or tissue-specific GAL4 drivers causes a reduction in synaptic branching and bouton number. We conclude that betaPS integrin at the postembryonic NMJ is a critical determinant of morphological growth and synaptic specificity. These data provide the first genetic evidence for a functional role of integrins at the postembryonic synapse.
This article has been cited by other articles:
|
|
 |
|
  H. Kohsaka, E. Takasu, and A. Nose In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2 J. Cell Biol., December 17, 2007; 179(6): 1289 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rohrbough, E. Rushton, E. Woodruff III, T. Fergestad, K. Vigneswaran, and K. Broadie Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation Genes & Dev., October 15, 2007; 21(20): 2607 - 2628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Besse, S. Mertel, R. J. Kittel, C. Wichmann, T. M. Rasse, S. J. Sigrist, and A. Ephrussi The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses J. Cell Biol., June 21, 2007; 177(5): 843 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Buff, A. C. Smith, and C. A. Korey Genetic Modifiers of Drosophila Palmitoyl-Protein Thioesterase 1-Induced Degeneration Genetics, May 1, 2007; 176(1): 209 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Huang, K. Shimazu, N. H. Woo, K. Zang, U. Muller, B. Lu, and L. F. Reichardt Distinct Roles of the beta1-Class Integrins at the Developing and the Mature Hippocampal Excitatory Synapse J. Neurosci., October 25, 2006; 26(43): 11208 - 11219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bhandari, J. W. Gargano, M. M. Goddeeris, and M. S. Grotewiel Behavioral Responses to Odorants in Drosophila Require Nervous System Expression of the {beta} Integrin Gene Myospheroid Chem Senses, September 1, 2006; 31(7): 627 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. I. Bassett, R. J. Bryson-Richardson, D. F. Daggett, P. Gautier, D. G. Keenan, and P. D. Currie Dystrophin is required for the formation of stable muscle attachments in the zebrafish embryo Development, December 1, 2003; 130(23): 5851 - 5860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Renden and K. Broadie Mutation and Activation of Galpha s Similarly Alters Pre- and Postsynaptic Mechanisms Modulating Neurotransmission J Neurophysiol, May 1, 2003; 89(5): 2620 - 2638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Ackley, S. H. Kang, J. R. Crew, C. Suh, Y. Jin, and J. M. Kramer The Basement Membrane Components Nidogen and Type XVIII Collagen Regulate Organization of Neuromuscular Junctions in Caenorhabditis elegans J. Neurosci., May 1, 2003; 23(9): 3577 - 3587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Beumer, H. J. G. Matthies, A. Bradshaw, and K. Broadie Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development Development, March 9, 2003; 129(14): 3381 - 3391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Featherstone, W. S. Davis, R. R. Dubreuil, and K. Broadie Drosophila {alpha}- and {beta}-Spectrin Mutations Disrupt Presynaptic Neurotransmitter Release J. Neurosci., June 15, 2001; 21(12): 4215 - 4224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rohrbough, M. S. Grotewiel, R. L. Davis, and K. Broadie Integrin-Mediated Regulation of Synaptic Morphology, Transmission, and Plasticity J. Neurosci., September 15, 2000; 20(18): 6868 - 6878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Sone, E Suzuki, M Hoshino, D Hou, H Kuromi, M Fukata, S Kuroda, K Kaibuchi, Y Nabeshima, and C Hama Synaptic development is controlled in the periactive zones of Drosophila synapses Development, January 10, 2000; 127(19): 4157 - 4168. [Abstract] [PDF]
|
|
