|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 5 987-998, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
R Crowe, J Zikherman and L Niswander
Cell Biology and Molecular Biology Programs, Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Endochondral bone development begins with the formation of a cartilage template. Chondrocytes within this template undergo a progressive program of maturation from proliferative to prehypertrophic chondrocytes to hypertrophic chondrocytes. The progression of cells through these steps of differentiation must be carefully controlled to ensure coordinated growth. Because the Delta/Notch signaling system is known to regulate cell fate choices, we sought to determine if these molecules might be involved in the progressive cell fate decisions that chondocytes undergo. Here we demonstrate in the chick that Delta/Notch signaling negatively regulates progression from the prehypertrophic to hypertrophic state of differentiation. Delta-1 is expressed specifically in the hypertrophic chondrocytes while Notch-2 is expressed in chondrocytes at all stages. Misexpression of Delta-1 using a replication-competent retrovirus blocks chondrocyte maturation. Prehypertrophic cells form normally but do not undergo differentiation to hypertrophic cells, resulting in shortened skeletal elements that lack ossification. We conclude that Delta-1 acts during chondrogenesis to inhibit the transition from prehypertrophic chondrocytes to hypertrophic chondrocytes, thus defining a novel mechanism for the regulation of the chondrocyte maturation program. In addition, these results reveal a new role for Delta/Notch signaling in regulating the progression to a terminally differentiated state.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. J. Mead and K. E. Yutzey Notch pathway regulation of chondrocyte differentiation and proliferation during appendicular and axial skeleton development PNAS, August 25, 2009; 106(34): 14420 - 14425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nakao, H. Kajiya, H. Fukushima, A. Fukushima, H. Anan, S. Ozeki, and K. Okabe PTHrP Induces Notch Signaling in Periodontal Ligament Cells Journal of Dental Research, June 1, 2009; 88(6): 551 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pascoal and I. Palmeirim Watch-ing out for chick limb development Integr. Comp. Biol., September 1, 2007; 47(3): 382 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Dowthwaite, J. C. Bishop, S. N. Redman, I. M. Khan, P. Rooney, D. J. R. Evans, L. Haughton, Z. Bayram, S. Boyer, B. Thomson, et al. The surface of articular cartilage contains a progenitor cell population J. Cell Sci., February 22, 2004; 117(6): 889 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, L. Topol, H. Lee, and J. Wu Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation Development, March 1, 2003; 130(5): 1003 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sakamoto, S. Yamaguchi, R. Ando, A. Miyawaki, Y. Kabasawa, M. Takagi, C. L. Li, B. Perbal, and K.-i. Katsube The Nephroblastoma Overexpressed Gene (NOV/ccn3) Protein Associates with Notch1 Extracellular Domain and Inhibits Myoblast Differentiation via Notch Signaling Pathway J. Biol. Chem., August 9, 2002; 277(33): 29399 - 29405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Piedra, M. D. Delgado, M. A. Ros, and J. Leon c-Myc Overexpression Increases Cell Size and Impairs Cartilage Differentiation during Chick Limb Development Cell Growth Differ., April 1, 2002; 13(4): 185 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Guo, B. Lanske, B.-Y. Liu, P. Divieti, H. M. Kronenberg, and F. R. Bringhurst Signal-Selectivity of Parathyroid Hormone (PTH)/PTH-Related Peptide Receptor-Mediated Regulation of Differentiation in Conditionally Immortalized Growth-Plate Chondrocytes Endocrinology, March 1, 2001; 142(3): 1260 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B Kerber, I Monge, M Mueller, P. Mitchell, and S. Cohen The AP-2 transcription factor is required for joint formation and cell survival in Drosophila leg development Development, January 4, 2001; 128(8): 1231 - 1238. [Abstract] [PDF]
|
|
|
|
|
|
M Delfini, E Hirsinger, O Pourquie, and D Duprez Delta 1-activated notch inhibits muscle differentiation without affecting Myf5 and Pax3 expression in chick limb myogenesis Development, January 12, 2000; 127(23): 5213 - 5224. [Abstract] [PDF]
|
|
|
|
|
|
M. Rones, K. McLaughlin, M Raffin, and M Mercola Serrate and Notch specify cell fates in the heart field by suppressing cardiomyogenesis Development, January 9, 2000; 127(17): 3865 - 3876. [Abstract] [PDF]
|
|
|
|
|
|
C Hartmann and C. Tabin Dual roles of Wnt signaling during chondrogenesis in the chicken limb Development, January 7, 2000; 127(14): 3141 - 3159. [Abstract] [PDF]
|
|
