|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 6 1189-1200, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
S Martinez, PH Crossley, I Cobos, JL Rubenstein and GR Martin
Department of Morphological Sciences, Faculty of Medicine, University of Murcia, Murcia, Spain.
Beads containing recombinant FGF8 (FGF8-beads) were implanted in the prospective caudal diencephalon or midbrain of chick embryos at stages 9-12. This induced the neuroepithelium rostral and caudal to the FGF8-bead to form two ectopic, mirror-image midbrains. Furthermore, cells in direct contact with the bead formed an outgrowth that protruded laterally from the neural tube. Tissue within such lateral outgrowths developed proximally into isthmic nuclei and distally into a cerebellum-like structure. These morphogenetic effects were apparently due to FGF8-mediated changes in gene expression in the vicinity of the bead, including a repressive effect on Otx2 and an inductive effect on En1, Fgf8 and Wnt1 expression. The ectopic Fgf8 and Wnt1 expression domains formed nearly complete concentric rings around the FGF8-bead, with the Wnt1 ring outermost. These observations suggest that FGF8 induces the formation of a ring-like ectopic signaling center (organizer) in the lateral wall of the brain, similar to the one that normally encircles the neural tube at the isthmic constriction, which is located at the boundary between the prospective midbrain and hindbrain. This ectopic isthmic organizer apparently sends long-range patterning signals both rostrally and caudally, resulting in the development of the two ectopic midbrains. Interestingly, our data suggest that these inductive signals spread readily in a caudal direction, but are inhibited from spreading rostrally across diencephalic neuromere boundaries. These results provide insights into the mechanism by which FGF8 induces an ectopic organizer and suggest that a negative feedback loop between Fgf8 and Otx2 plays a key role in patterning the midbrain and anterior hindbrain.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Sato and A. L. Joyner The duration of Fgf8 isthmic organizer expression is key to patterning different tectal-isthmo-cerebellum structures Development, November 1, 2009; 136(21): 3617 - 3626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Martinez-Ferre and S. Martinez The Development of the Thalamic Motor Learning Area Is Regulated by Fgf8 Expression J. Neurosci., October 21, 2009; 29(42): 13389 - 13400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Paek, G. Gutin, and J. M. Hebert FGF signaling is strictly required to maintain early telencephalic precursor cell survival Development, July 15, 2009; 136(14): 2457 - 2465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamauchi, S. Mizushima, A. Tamada, N. Yamamoto, S. Takashima, and F. Murakami FGF8 Signaling Regulates Growth of Midbrain Dopaminergic Axons by Inducing Semaphorin 3F J. Neurosci., April 1, 2009; 29(13): 4044 - 4055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kataoka and T. Shimogori Fgf8 controls regional identity in the developing thalamus Development, September 1, 2008; 135(17): 2873 - 2881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Badde and D. Schulte A Role for Receptor Protein Tyrosine Phosphatase {lambda} in Midbrain Development J. Neurosci., June 11, 2008; 28(24): 6152 - 6164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Basson, D. Echevarria, C. Petersen Ahn, A. Sudarov, A. L. Joyner, I. J. Mason, S. Martinez, and G. R. Martin Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development Development, March 1, 2008; 135(5): 889 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Carletti and F. Rossi Neurogenesis in the Cerebellum Neuroscientist, February 1, 2008; 14(1): 91 - 100. [Abstract] [PDF]
|
|
|
|
 |
|
 E. Salero and M. E. Hatten Differentiation of ES cells into cerebellar neurons PNAS, February 20, 2007; 104(8): 2997 - 3002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fogarty, B. A. Emmenegger, L. L. Grasfeder, T. G. Oliver, and R. J. Wechsler-Reya Fibroblast growth factor blocks Sonic hedgehog signaling in neuronal precursors and tumor cells PNAS, February 20, 2007; 104(8): 2973 - 2978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Guo, H.-Y. Qiu, Y. Huang, H. Chen, R.-Q. Yang, S.-D. Chen, R. L. Johnson, Z.-F. Chen, and Y.-Q. Ding Lmx1b is essential for Fgf8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice Development, January 15, 2007; 134(2): 317 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Morales and M. E. Hatten Molecular Markers of Neuronal Progenitors in the Embryonic Cerebellar Anlage. J. Neurosci., November 22, 2006; 26(47): 12226 - 12236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Alexandre, I. Bachy, M. Marcou, and M. Wassef Positive and negative regulations by FGF8 contribute to midbrain roof plate developmental plasticity Development, August 1, 2006; 133(15): 2905 - 2913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Koster and S. E. Fraser FGF signaling mediates regeneration of the differentiating cerebellum through repatterning of the anterior hindbrain and reinitiation of neuronal migration. J. Neurosci., July 5, 2006; 26(27): 7293 - 7304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, O. A. Ibrahimi, S. K. Olsen, H. Umemori, M. Mohammadi, and D. M. Ornitz Receptor Specificity of the Fibroblast Growth Factor Family: THE COMPLETE MAMMALIAN FGF FAMILY J. Biol. Chem., June 9, 2006; 281(23): 15694 - 15700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Foucher, M. Mione, A. Simeone, D. Acampora, L. Bally-Cuif, and C. Houart Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants Development, May 15, 2006; 133(10): 1891 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Storm, S. Garel, U. Borello, J. M. Hebert, S. Martinez, S. K. McConnell, G. R. Martin, and J. L. R. Rubenstein Dose-dependent functions of Fgf8 in regulating telencephalic patterning centers Development, May 1, 2006; 133(9): 1831 - 1844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Waters and M. Lewandoski A threshold requirement for Gbx2 levels in hindbrain development. Development, May 1, 2006; 133(10): 1991 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Griesel, D. Treichel, P. Collombat, J. Krull, A. Zembrzycki, W. M. R. van den Akker, P. Gruss, A. Simeone, and A. Mansouri Sp8 controls the anteroposterior patterning at the midbrain-hindbrain border Development, May 1, 2006; 133(9): 1779 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Picker and M. Brand Fgf signals from a novel signaling center determine axial patterning of the prospective neural retina Development, November 15, 2005; 132(22): 4951 - 4962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. O'Hara, E. Beck, L. K. Barr, L. L. Wong, D. S. Kessler, and R. D. Riddle Zebrafish Lmx1b.1 and Lmx1b.2 are required for maintenance of the isthmic organizer Development, July 15, 2005; 132(14): 3163 - 3173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Langenberg and M. Brand Lineage restriction maintains a stable organizer cell population at the zebrafish midbrain-hindbrain boundary Development, July 15, 2005; 132(14): 3209 - 3216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kimura, Y. Suda, D. Kurokawa, Z. M. Hossain, M. Nakamura, M. Takahashi, A. Hara, and S. Aizawa Emx2 and Pax6 Function in Cooperation with Otx2 and Otx1 to Develop Caudal Forebrain Primordium That Includes Future Archipallium J. Neurosci., May 25, 2005; 25(21): 5097 - 5108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. H. Li, Z. Lao, and A. L. Joyner New regulatory interactions and cellular responses in the isthmic organizer region revealed by altering Gbx2 expression Development, April 15, 2005; 132(8): 1971 - 1981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suzuki-Hirano, T. Sato, and H. Nakamura Regulation of isthmic Fgf8 signal by sprouty2 Development, January 15, 2005; 132(2): 257 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hans, D. Liu, and M. Westerfield Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors Development, October 15, 2004; 131(20): 5091 - 5102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato and H. Nakamura The Fgf8 signal causes cerebellar differentiation by activating the Ras-ERK signaling pathway Development, September 1, 2004; 131(17): 4275 - 4285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kurokawa, H. Kiyonari, R. Nakayama, C. Kimura-Yoshida, I. Matsuo, and S. Aizawa Regulation of Otx2 expression and its functions in mouse forebrain and midbrain Development, July 15, 2004; 131(14): 3319 - 3331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tabata and Y. Takei Morphogens, their identification and regulation Development, February 15, 2004; 131(4): 703 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jaszai, F. Reifers, A. Picker, T. Langenberg, and M. Brand Isthmus-to-midbrain transformation in the absence of midbrain-hindbrain organizer activity Development, December 29, 2003; 130(26): 6611 - 6623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liu, J. Y. H. Li, C. Bromleigh, Z. Lao, L. A. Niswander, and A. L. Joyner FGF17b and FGF18 have different midbrain regulatory properties from FGF8b or activated FGF receptors Development, December 22, 2003; 130(25): 6175 - 6185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Louvi, P. Alexandre, C. Metin, W. Wurst, and M. Wassef The isthmic neuroepithelium is essential for cerebellar midline fusion Development, November 15, 2003; 130(22): 5319 - 5330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Alexandre and M. Wassef The isthmic organizer links anteroposterior and dorsoventral patterning in the mid/hindbrain by generating roof plate structures Development, November 15, 2003; 130(22): 5331 - 5338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tallafuss and L. Bally-Cuif Tracing of her5 progeny in zebrafish transgenics reveals the dynamics of midbrain-hindbrain neurogenesis and maintenance Development, September 15, 2003; 130(18): 4307 - 4323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Walshe and I. Mason Unique and combinatorial functions of Fgf3 and Fgf8 during zebrafish forebrain development Development, September 15, 2003; 130(18): 4337 - 4349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Chi, S. Martinez, W. Wurst, and G. R. Martin The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum Development, June 15, 2003; 130(12): 2633 - 2644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Hirth, L. Kammermeier, E. Frei, U. Walldorf, M. Noll, and H. Reichert An urbilaterian origin of the tripartite brain: developmental genetic insights from Drosophila Development, June 1, 2003; 130(11): 2365 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garel, K. J. Huffman, and J. L. R. Rubenstein Molecular regionalization of the neocortex is disrupted in Fgf8 hypomorphic mutants Development, May 1, 2003; 130(9): 1903 - 1914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dono Fibroblast growth factors as regulators of central nervous system development and function Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2003; 284(4): R867 - R881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Matsunaga, T. Katahira, and H. Nakamura Role of Lmx1b and Wnt1 in mesencephalon and metencephalon development Development, March 13, 2003; 129(22): 5269 - 5277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Reim and M. Brand spiel-ohne-grenzen/pou2 mediates regional competence to respond to Fgf8 during zebrafish early neural development Development, March 4, 2003; 129(4): 917 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lutolf, F. Radtke, M. Aguet, U. Suter, and V. Taylor Notch1 is required for neuronal and glial differentiation in the cerebellum Development, March 3, 2003; 129(2): 373 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ford-Perriss, S. E. Guimond, U. Greferath, M. Kita, K. Grobe, H. Habuchi, K. Kimata, J. D. Esko, M. Murphy, and J. E. Turnbull Variant heparan sulfates synthesized in developing mouse brain differentially regulate FGF signaling Glycobiology, November 1, 2002; 12(11): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Irving, A. Malhas, S. Guthrie, and I. Mason Establishing the trochlear motor axon trajectory: role of the isthmic organiser and Fgf8 Development, January 12, 2002; 129(23): 5389 - 5398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Glavic, J. L. Gomez-Skarmeta, and R. Mayor The homeoprotein Xiro1 is required for midbrain-hindbrain boundary formation Development, January 4, 2002; 129(7): 1609 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kobayashi, M. Kobayashi, K. Matsumoto, T. Ogura, M. Nakafuku, and K. Shimamura Early subdivisions in the neural plate define distinct competence for inductive signals Development, January 1, 2002; 129(1): 83 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. H. Li and A. L. Joyner Otx2 and Gbx2 are required for refinement and not induction of mid-hindbrain gene expression Development, December 15, 2001; 128(24): 4979 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Martinez-Barbera, M. Signore, P. P. Boyl, E. Puelles, D. Acampora, R. Gogoi, F. Schubert, A. Lumsden, and A. Simeone Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2 Development, December 1, 2001; 128(23): 4789 - 4800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-G. Belting, G. Hauptmann, D. Meyer, S. Abdelilah-Seyfried, A. Chitnis, C. Eschbach, I. Soll, C. Thisse, B. Thisse, K. B. Artinger, et al. spiel ohne grenzen/pou2 is required during establishment of the zebrafish midbrain-hindbrain boundary organizer Development, November 1, 2001; 128(21): 4165 - 4176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Matsunaga, I. Araki, and H. Nakamura Role of Pax3/7 in the tectum regionalization Development, October 15, 2001; 128(20): 4069 - 4077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Schneider, D. Hu, J. L. R. Rubenstein, M. Maden, and J. A. Helms Local retinoid signaling coordinates forebrain and facial morphogenesis by maintaining FGF8 and SHH Development, July 15, 2001; 128(14): 2755 - 2767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, I. Araki, and H. Nakamura Inductive signal and tissue responsiveness defining the tectum and the cerebellum Development, July 1, 2001; 128(13): 2461 - 2469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Liu and A. Joyner EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region Development, January 1, 2001; 128(2): 181 - 191. [Abstract] [PDF]
|
|
|
|
|
|
F. M. Vaccarino Stem Cells and Neuronal Progenitors and Their Diversity in the CNS: Are Time and Place Important? Neuroscientist, October 1, 2000; 6(5): 338 - 352. [Abstract] [PDF]
|
|
|
|
|
|
R. Raballo, J. Rhee, R. Lyn-Cook, J. F. Leckman, M. L. Schwartz, and F. M. Vaccarino Basic Fibroblast Growth Factor (Fgf2) Is Necessary for Cell Proliferation and Neurogenesis in the Developing Cerebral Cortex J. Neurosci., July 1, 2000; 20(13): 5012 - 5023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Bertrand, F Medevielle, and F Pituello FGF signalling controls the timing of Pax6 activation in the neural tube Development, January 11, 2000; 127(22): 4837 - 4843. [Abstract] [PDF]
|
|
|
|
|
|
F Marin and P Charnay Hindbrain patterning: FGFs regulate Krox20 and mafB/kr expression in the otic/preotic region Development, January 11, 2000; 127(22): 4925 - 4935. [Abstract] [PDF]
|
|
|
|
|
|
J. Martinez Barbera, M Clements, P Thomas, T Rodriguez, D Meloy, D Kioussis, and R. Beddington The homeobox gene Hex is required in definitive endodermal tissues for normal forebrain, liver and thyroid formation Development, January 6, 2000; 127(11): 2433 - 2445. [Abstract] [PDF]
|
|
|
|
|
|
J Xu, Z Liu, and D. Ornitz Temporal and spatial gradients of Fgf8 and Fgf17 regulate proliferation and differentiation of midline cerebellar structures Development, January 5, 2000; 127(9): 1833 - 1843. [Abstract] [PDF]
|
|
|
|
|
|
K. Adams, J. Maida, J. Golden, and R. Riddle The transcription factor Lmx1b maintains Wnt1 expression within the isthmic organizer Development, January 5, 2000; 127(9): 1857 - 1867. [Abstract] [PDF]
|
|
|
|
|
|
T Nomura and H Fujisawa Alteration of the retinotectal projection map by the graft of mesencephalic floor plate or sonic hedgehog Development, January 5, 2000; 127(9): 1899 - 1910. [Abstract] [PDF]
|
|
|
|
|
|
C Irving and I Mason Signalling by FGF8 from the isthmus patterns anterior hindbrain and establishes the anterior limit of Hox gene expression Development, January 1, 2000; 127(1): 177 - 186. [Abstract] [PDF]
|
|
|
|
 |
|
 K. J. Millen, J. H. Millonig, R. J. T. Wingate, J. Alder, and M. E. Hatten Neurogenetics of the Cerebellar System J Child Neurol, September 1, 1999; 14(9): 574 - 581. [Abstract] [PDF]
|
|
|
|
|
|
M Carl and J Wittbrodt Graded interference with FGF signalling reveals its dorsoventral asymmetry at the mid-hindbrain boundary Development, January 12, 1999; 126(24): 5659 - 5667. [Abstract] [PDF]
|
|
|
|
|
|
A Liu, K Losos, and A. Joyner FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fate Development, January 11, 1999; 126(21): 4827 - 4838. [Abstract] [PDF]
|
|
|
|
|
|
F Ristoratore, M Carl, K Deschet, L Richard-Parpaillon, D Boujard, J Wittbrodt, D Chourrout, F Bourrat, and J. Joly The midbrain-hindbrain boundary genetic cascade is activated ectopically in the diencephalon in response to the widespread expression of one of its components, the medaka gene Ol-eng2 Development, January 9, 1999; 126(17): 3769 - 3779. [Abstract] [PDF]
|
|
|
|
|
|
C Irving and I Mason Regeneration of isthmic tissue is the result of a specific and direct interaction between rhombomere 1 and midbrain Development, January 9, 1999; 126(18): 3981 - 3989. [Abstract] [PDF]
|
|
|
|
|
|
M Hidalgo-Sanchez, A Simeone, and R. Alvarado-Mallart Fgf8 and Gbx2 induction concomitant with Otx2 repression is correlated with midbrain-hindbrain fate of caudal prosencephalon Development, January 6, 1999; 126(14): 3191 - 3203. [Abstract] [PDF]
|
|
