Development, Vol 126, Issue 8 1739-1751, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Tbx5 is essential for heart development

ME Horb and GH Thomsen
Department of Biochemistry and Cell Biology, Institute of Cell and Developmental Biology, State University of New York, Stony Brook, NY 11794-5215, USA. gthomsen@notes.cc.sunysb.edu

Mutations in the Tbx5 transcription factor cause heart septal defects found in human Holt-Oram Syndrome. The complete extent to which Tbx5 functions in heart development, however, has not been established. Here we show that, in Xenopus embryos, Tbx5 is expressed in the early heart field, posterior to the cardiac homeobox transcription factor, Nkx2.5. During morphogenesis, Tbx5 is expressed throughout the heart tube except the anterior portion, the bulbus cordis. When Tbx5 activity is antagonized with a hormone-inducible, dominant negative version of the protein, the heart fails to develop. These results suggest that, in addition to its function in heart septation, Tbx5 has a more global role in cardiac specification and heart development in vertebrate embryos.
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