|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 10 2041-2051, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
AK Corsi, SA Kostas, A Fire and M Krause
Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, MD 20892-0510, USA.
The basic helix-loop-helix (bHLH) transcription factor Twist plays a role in mesodermal development in both invertebrates and vertebrates. In an effort to understand the role of the unique Caenorhabditis elegans Twist homolog, hlh-8, we analyzed mesodermal development in animals with a deletion in the hlh-8 locus. This deletion was predicted to represent a null allele because the HLH domain is missing and the reading frame for the protein is disrupted. Animals lacking CeTwist function were constipated and egg-laying defective. Both of these defects were rescued in transgenic mutant animals expressing wild-type hlh-8. Observing a series of mesoderm-specific markers allowed us to characterize the loss of hlh-8 function more thoroughly. Our results demonstrate that CeTwist performs an essential role in the proper development of a subset of mesodermal tissues in C. elegans. We found that CeTwist was required for the formation of three out of the four non-striated enteric muscles born in the embryo. In contrast, CeTwist was not required for the formation of the embryonically derived striated muscles. Most of the post-embryonic mesoderm develops from a single lineage. CeTwist was necessary for appropriate patterning in this lineage and was required for expression of two downstream target genes, but was not required for the expression of myosin, a marker of differentiation. Our results suggest that mesodermal patterning by Twist is an evolutionarily conserved function.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  N. M. Amin, K. Hu, D. Pruyne, D. Terzic, A. Bretscher, and J. Liu A Zn-finger/FH2-domain containing protein, FOZI-1, acts redundantly with CeMyoD to specify striated body wall muscle fates in the Caenorhabditis elegans postembryonic mesoderm Development, January 1, 2007; 134(1): 19 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Branicky and S. Hekimi Specification of muscle neurotransmitter sensitivity by a Paired-like homeodomain protein in Caenorhabditis elegans Development, November 15, 2005; 132(22): 4999 - 5009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang, V. Horner, and J. Liu The HMX homeodomain protein MLS-2 regulates cleavage orientation, cell proliferation and cell fate specification in the C. elegans postembryonic mesoderm Development, September 15, 2005; 132(18): 4119 - 4130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fukushige and M. Krause The myogenic potency of HLH-1 reveals wide-spread developmental plasticity in early C. elegans embryos Development, April 15, 2005; 132(8): 1795 - 1805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Deplancke, D. Dupuy, M. Vidal, and A. J.M. Walhout A Gateway-Compatible Yeast One-Hybrid System Genome Res., October 1, 2004; 14(10b): 2093 - 2101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mehta, P. M. Loria, and O. Hobert A Genetic Screen for Neurite Outgrowth Mutants in Caenorhabditis elegans Reveals a New Function for the F-box Ubiquitin Ligase Component LIN-23 Genetics, March 1, 2004; 166(3): 1253 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kostrouchova, Z. Kostrouch, V. Saudek, J. Piatigorsky, and J. E. Rall BIR-1, a Caenorhabditis elegans homologue of Survivin, regulates transcription and development PNAS, April 29, 2003; 100(9): 5240 - 5245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Kostas and A. Fire The T-box factor MLS-1 acts as a molecular switch during specification of nonstriated muscle in C. elegans Genes & Dev., January 15, 2002; 16(2): 257 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Corsi, T. M. Brodigan, E. M. Jorgensen, and M. Krause Characterization of a dominant negative C. elegans Twist mutant protein with implications for human Saethre-Chotzen syndrome Development, January 6, 2002; 129(11): 2761 - 2772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Nguyen, J. Round, R. O'Connell, P. Geurts, M. Funes-Duran, J. Wong, G. Jongeward, and C. A. Vierra Isolation and characterization of the activated B-cell factor 1 homolog in Caenorhabditis elegans Nucleic Acids Res., November 1, 2001; 29(21): 4423 - 4432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Jungblut, A. Pires-daSilva, and R. J. Sommer Formation of the egg-laying system in Pristionchus pacificus requires complex interactions between gonadal, mesodermal and epidermal tissues and does not rely on single cell inductions Development, September 15, 2001; 128(18): 3395 - 3404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kostrouchova, M. Krause, Z. Kostrouch, and J. E. Rall Nuclear hormone receptor CHR3 is a critical regulator of all four larval molts of the nematode Caenorhabditis elegans PNAS, June 19, 2001; 98(13): 7360 - 7365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Liu and A Fire Overlapping roles of two Hox genes and the exd ortholog ceh-20 in diversification of the C. elegans postembryonic mesoderm Development, January 12, 2000; 127(23): 5179 - 5190. [Abstract] [PDF]
|
|
|
|
|
|
D. Portman and S. Emmons The basic helix-loop-helix transcription factors LIN-32 and HLH-2 function together in multiple steps of a C. elegans neuronal sublineage Development, January 12, 2000; 127(24): 5415 - 5426. [Abstract] [PDF]
|
|
