|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 11 2503-2514, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
SI Osada, Y Saijoh, A Frisch, CY Yeo, H Adachi, M Watanabe, M Whitman, H Hamada and CV Wright
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2175, USA.
Vertebrate Nodal-related factors play central roles in mesendoderm induction and left-right axis specification, but the mechanisms regulating their expression are largely unknown. We identify an element in Xnr1 intron 1 that is activated by activin and Vg1, autoactivated by Xnrs, and suppressed by ventral inducers like BMP4. Intron 1 contains three FAST binding sites on which FAST/Smad transcriptional complexes can assemble; these sites are differentially involved in intron 1-mediated reporter gene expression. Interference with FAST function abolishes intron 1 activity, and transcriptional activation of Xnrs by activin in embryonic tissue explant assays, identifying FAST as an essential mediator of Xnr autoregulation and/or 'signal relay' from activin-like molecules. Furthermore, the mapping of endogenous activators of the Xnr1 intronic enhancer within Xenopus embryos agrees well with the pattern of Xnr1 transcription during embryogenesis. In transgenic mice, Xnr1 intron 1 mimics a similarly located enhancer in the mouse nodal gene, and directs FAST site-dependent expression in the primitive streak during gastrulation, and unilateral expression during early somitogenesis. The FAST cassette is similar in an ascidian nodal-related gene, suggesting an ancient origin for this regulatory module. Thus, an evolutionarily conserved intronic enhancer in Xnr1 is involved in both mesendoderm induction and asymmetric expression during left-right axis formation.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. L. Blount, J. M. Vaughan, W. W. Vale, and L. M. Bilezikjian A Smad-binding Element in Intron 1 Participates in Activin-dependent Regulation of the Follistatin Gene J. Biol. Chem., March 14, 2008; 283(11): 7016 - 7026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Range, F. Lapraz, M. Quirin, S. Marro, L. Besnardeau, and T. Lepage Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-{beta} related to Vg1 Development, October 15, 2007; 134(20): 3649 - 3664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Tavares, S. Andrade, A. C. Silva, and J. A. Belo Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo Development, June 1, 2007; 134(11): 2051 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Peeters, M. L. Voz, K. Verschueren, B. De Cat, H. Pendeville, B. Thienpont, A. Schellens, J. W. Belmont, G. David, W. J.M. Van De Ven, et al. Sesn1 is a novel gene for left-right asymmetry and mediating nodal signaling Hum. Mol. Genet., November 15, 2006; 15(22): 3369 - 3377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ishimura, J. K. Ng, M. Taira, S. G. Young, and S.-I. Osada Man1, an inner nuclear membrane protein, regulates vascular remodeling by modulating transforming growth factor {beta} signaling Development, October 1, 2006; 133(19): 3919 - 3928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Zamparini, T. Watts, C. E. Gardner, S. R. Tomlinson, G. I. Johnston, and J. M. Brickman Hex acts with {beta}-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal Development, September 15, 2006; 133(18): 3709 - 3722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Onuma, C.-Y. Yeo, and M. Whitman XCR2, one of three Xenopus EGF-CFC genes, has a distinct role in the regulation of left-right patterning Development, January 15, 2006; 133(2): 237 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jullien and J. Gurdon Morphogen gradient interpretation by a regulated trafficking step during ligand-receptor transduction Genes & Dev., November 15, 2005; 19(22): 2682 - 2694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Houston and C. Wylie Maternal Xenopus Zic2 negatively regulates Nodal-related gene expression during anteroposterior patterning Development, November 1, 2005; 132(21): 4845 - 4855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ku, S. Y. Sokol, J. Wu, M. I. Tussie-Luna, A. L. Roy, and A. Hata Positive and Negative Regulation of the Transforming Growth Factor {beta}/Activin Target Gene goosecoid by the TFII-I Family of Transcription Factors Mol. Cell. Biol., August 15, 2005; 25(16): 7144 - 7157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Dunn, C. H. Koonce, D. C. Anderson, A. Islam, E. K. Bikoff, and E. J. Robertson Mice exclusively expressing the short isoform of Smad2 develop normally and are viable and fertile Genes & Dev., January 1, 2005; 19(1): 152 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kofron, H. Puck, H. Standley, C. Wylie, R. Old, M. Whitman, and J. Heasman New roles for FoxH1 in patterning the early embryo Development, October 15, 2004; 131(20): 5065 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, H. Zhou, Y. Su, Z. Sun, H. Zhang, L. Zhang, Y. Zhang, Y. Ning, Y.-G. Chen, and A. Meng Zebrafish Dpr2 Inhibits Mesoderm Induction by Promoting Degradation of Nodal Receptors Science, October 1, 2004; 306(5693): 114 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hashimoto, M. Rebagliati, N. Ahmad, O. Muraoka, T. Kurokawa, M. Hibi, and T. Suzuki The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish Development, April 15, 2004; 131(8): 1741 - 1753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Kunwar, S. Zimmerman, J. T. Bennett, Y. Chen, M. Whitman, and A. F. Schier Mixer/Bon and FoxH1/Sur have overlapping and divergent roles in Nodal signaling and mesendoderm induction Development, December 1, 2003; 130(23): 5589 - 5599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Long, N. Ahmad, and M. Rebagliati The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry Development, June 1, 2003; 130(11): 2303 - 2316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Dougan, R. M. Warga, D. A. Kane, A. F. Schier, and W. S. Talbot The role of the zebrafish nodal-related genes squint and cyclops in patterning of mesendoderm Development, May 1, 2003; 130(9): 1837 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Howell, G. J. Inman, and C. S. Hill A novel Xenopus Smad-interacting forkhead transcription factor (XFast-3) cooperates with XFast-1 in regulating gastrulation movements Development, March 8, 2003; 129(12): 2823 - 2834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Shim, N. Bae, and J.-K. Han Bone morphogenetic protein-4-induced activation of Xretpos is mediated by Smads and Olf-1/EBF associated zinc finger (OAZ) Nucleic Acids Res., July 15, 2002; 30(14): 3107 - 3117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-T. Yan, J.-J. Liu, Y. Luo, C. E, R. S. Haltiwanger, C. Abate-Shen, and M. M. Shen Dual Roles of Cripto as a Ligand and Coreceptor in the Nodal Signaling Pathway Mol. Cell. Biol., July 1, 2002; 22(13): 4439 - 4449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ring, S. Ogata, L. Meek, J. Song, T. Ohta, K. Miyazono, and K. W.Y. Cho The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-containing transcription factor in activin/nodal signaling Genes & Dev., April 1, 2002; 16(7): 820 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Eimon and R. M. Harland Effects of heterodimerization and proteolytic processing on Derriere and Nodal activity: implications for mesoderm induction in Xenopus Development, January 7, 2002; 129(13): 3089 - 3103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Yamamoto, C. Takagi, A. C. Hyodo, and N. Ueno Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling Development, July 15, 2001; 128(14): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, C. Meno, Y. Sakai, H. Shiratori, K. Mochida, Y. Ikawa, Y. Saijoh, and H. Hamada The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse Genes & Dev., May 15, 2001; 15(10): 1242 - 1256. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. Hoodless, M. Pye, C. Chazaud, E. Labbé, L. Attisano, J. Rossant, and J. L. Wrana FoxH1 (Fast) functions to specify the anterior primitive streak in the mouse Genes & Dev., May 15, 2001; 15(10): 1257 - 1271. [Abstract] [Full Text]
|
|
|
|
|
|
S Takahashi, C Yokota, K Takano, K Tanegashima, Y Onuma, J Goto, and M Asashima Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center Development, January 12, 2000; 127(24): 5319 - 5329. [Abstract] [PDF]
|
|
