|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 17 3855-3864, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
JA Brill, GR Hime, M Scharer-Schuksz and MT Fuller
Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA. brill@u.washington.edu
The endgame of cytokinesis can follow one of two pathways depending on developmental context: resolution into separate cells or formation of a stable intercellular bridge. Here we show that the four wheel drive (fwd) gene of Drosophila melanogaster is required for intercellular bridge formation during cytokinesis in male meiosis. In fwd mutant males, contractile rings form and constrict in dividing spermatocytes, but cleavage furrows are unstable and daughter cells fuse together, producing multinucleate spermatids. fwd is shown to encode a phosphatidylinositol 4-kinase (PI 4-kinase), a member of a family of proteins that perform the first step in the synthesis of the key regulatory membrane phospholipid PIP2. Wild-type activity of the fwd PI 4-kinase is required for tyrosine phosphorylation in the cleavage furrow and for normal organization of actin filaments in the constricting contractile ring. Our results suggest a critical role for PI 4-kinases and phosphatidylinositol derivatives during the final stages of cytokinesis.
This article has been cited by other articles:
|
|
 |
|
  W.-Q. Yang, Y. Lai, M.-N. Li, W.-Y. Xu, and Y.-B. Xue A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice Mol Plant, July 21, 2008; (2008) ssn035v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wei, J. Rollins, L. Fabian, M. Hayes, G. Polevoy, C. Bazinet, and J. A. Brill Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis J. Cell Sci., April 1, 2008; 121(7): 1076 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dyer, E. Rebollo, P. Dominguez, N. Elkhatib, P. Chavrier, L. Daviet, C. Gonzalez, and M. Gonzalez-Gaitan Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes Development, December 15, 2007; 134(24): 4437 - 4447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Giansanti, G. Belloni, and M. Gatti Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males Mol. Biol. Cell, December 1, 2007; 18(12): 5034 - 5047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Bicknell, A. Babour, C. M. Federovitch, and M. Niwa A novel role in cytokinesis reveals a housekeeping function for the unfolded protein response J. Cell Biol., July 30, 2007; 177(6): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Rodgers, J. P. Albanesi, and M. A. Phillips Phosphatidylinositol 4-Kinase III-{beta} Is Required for Golgi Maintenance and Cytokinesis in Trypanosoma brucei Eukaryot. Cell, July 1, 2007; 6(7): 1108 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Janetopoulos and P. Devreotes Phosphoinositide signaling plays a key role in cytokinesis J. Cell Biol., August 14, 2006; 174(4): 485 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Gatt and D. M. Glover The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis J. Cell Sci., June 1, 2006; 119(11): 2225 - 2235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Dowd, S. Coursol, A. L. Skirpan, T.-h. Kao, and S. Gilroy Petunia Phospholipase C1 Is Involved in Pollen Tube Growth PLANT CELL, June 1, 2006; 18(6): 1438 - 1453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Greenbaum, W. Yan, M.-H. Wu, Y.-N. Lin, J. E. Agno, M. Sharma, R. E. Braun, A. Rajkovic, and M. M. Matzuk TEX14 is essential for intercellular bridges and fertility in male mice PNAS, March 28, 2006; 103(13): 4982 - 4987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Emoto, H. Inadome, Y. Kanaho, S. Narumiya, and M. Umeda Local Change in Phospholipid Composition at the Cleavage Furrow Is Essential for Completion of Cytokinesis J. Biol. Chem., November 11, 2005; 280(45): 37901 - 37907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lord and T. D. Pollard UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II J. Cell Biol., October 25, 2004; 167(2): 315 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saul, L. Fabian, A. Forer, and J. A. Brill Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes J. Cell Sci., August 1, 2004; 117(17): 3887 - 3896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Wakimoto, D. L. Lindsley, and C. Herrera Toward a Comprehensive Genetic Analysis of Male Fertility in Drosophila melanogaster Genetics, May 1, 2004; 167(1): 207 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Giansanti, R. M. Farkas, S. Bonaccorsi, D. L. Lindsley, B. T. Wakimoto, M. T. Fuller, and M. Gatti Genetic Dissection of Meiotic Cytokinesis in Drosophila Males Mol. Biol. Cell, May 1, 2004; 15(5): 2509 - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D. Cunto, S. Imarisio, P. Camera, C. Boitani, F. Altruda, and L. Silengo Essential role of citron kinase in cytokinesis of spermatogenic precursors J. Cell Sci., March 14, 2003; 115(24): 4819 - 4826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Barylko, P. Wlodarski, D. D. Binns, S. H. Gerber, S. Earnest, T. C. Sudhof, N. Grichine, and J. P. Albanesi Analysis of the Catalytic Domain of Phosphatidylinositol 4-Kinase Type II J. Biol. Chem., November 8, 2002; 277(46): 44366 - 44375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Mueller-Roeber and C. Pical Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C Plant Physiology, September 1, 2002; 130(1): 22 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Somma, B. Fasulo, G. Cenci, E. Cundari, and M. Gatti Molecular Dissection of Cytokinesis by RNA Interference in Drosophila Cultured Cells Mol. Biol. Cell, July 1, 2002; 13(7): 2448 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kuriyama, C. Gustus, Y. Terada, Y. Uetake, and J. Matuliene CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis J. Cell Biol., March 4, 2002; 156(5): 783 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Sisson, C. Field, R. Ventura, A. Royou, and W. Sullivan Lava Lamp, a Novel Peripheral Golgi Protein, Is Required for Drosophila melanogaster Cellularization J. Cell Biol., November 13, 2000; 151(4): 905 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kuriyama, C. Gustus, Y. Terada, Y. Uetake, and J. Matuliene CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis J. Cell Biol., March 4, 2002; 156(5): 783 - 790. [Abstract] [Full Text] [PDF]
|
|
