|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 18 3981-3992, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
W Bender and A Hudson
BCMP Department, Harvard Medical School, Boston, MA 02115, USA.
P elements containing a 7 kb DNA fragment from the middle of the Drosophila bithorax complex insert preferentially into the bithorax complex or into the adjacent chromosome regions. This 'homing' property is similar to that reported for the engrailed promoter (Hama, C., Ali, Z. and Kornberg, T. B. (1990) Genes Dev. 4, 1079-1093). The 7 kb fragment does not contain any known promoter, but it acts as a boundary element separating adjacent segmental domains. An enhancer-trap P element was constructed with the homing fragment and the selectable marker flanked by FRT sites. P insertions can be trimmed down by Flp-mediated recombination to just the lacZ reporter, so that the (beta)-galactosidase pattern is not influenced by sequences inside the P element. Twenty insertions into the bithorax complex express (beta)-galactosidase in segmentally limited patterns, reflecting the segmental domains of the bithorax complex where the elements reside. The mapping of segmental domains has now been revised, with enlargement of the abx/bx, bxd/pbx, and the iab-3 domains. The FRT sites in the P elements permit recombination between pairs of elements on opposite chromosomes, to generate duplications or deletions of the DNA between the two insertion sites. Using this technique, the length of the Ultrabithorax transcription unit was varied from 37 to 138 kb, but there was surprisingly little effect on Ultrabithorax function.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Kwon, D. Mucci, K. K. Langlais, J. L. Americo, S. K. DeVido, Y. Cheng, and J. A. Kassis Enhancer-promoter communication at the Drosophila engrailed locus Development, September 15, 2009; 136(18): 3067 - 3075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fujioka, X. Wu, and J. B. Jaynes A chromatin insulator mediates transgene homing and very long-range enhancer-promoter communication Development, September 15, 2009; 136(18): 3077 - 3087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Garaulet, D. Foronda, M. Calleja, and E. Sanchez-Herrero Polycomb-dependent Ultrabithorax Hox gene silencing induced by high Ultrabithorax levels in Drosophila Development, October 1, 2008; 135(19): 3219 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Bender MicroRNAs in the Drosophila bithorax complex Genes & Dev., January 1, 2008; 22(1): 14 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ogasawara, H. Furuhashi, and S. Hirose DNA supercoiling factor positively regulates expression of the homeotic gene Abdominal-B in Drosophila melanogaster. Genes Cells, December 1, 2007; 12(12): 1347 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sipos, G. Kozma, E. Molnar, and W. Bender In situ dissection of a Polycomb response element in Drosophila melanogaster PNAS, July 24, 2007; 104(30): 12416 - 12421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mihaly, S. Barges, L. Sipos, R. Maeda, F. Cleard, I. Hogga, W. Bender, H. Gyurkovics, and F. Karch Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex Development, August 1, 2006; 133(15): 2983 - 2993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Maeda and F. Karch The ABC of the BX-C: the bithorax complex explained. Development, April 1, 2006; 133(8): 1413 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Foronda, B. Estrada, L. de Navas, and E. Sanchez-Herrero Requirement of abdominal-A and Abdominal-B in the developing genitalia of Drosophila breaks the posterior downregulation rule Development, January 1, 2006; 133(1): 117 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Hittinger, D. L. Stern, and S. B. Carroll Pleiotropic functions of a conserved insect-specific Hox peptide motif Development, December 1, 2005; 132(23): 5261 - 5270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Lin, Q. Chen, L. Lin, and J. Zhou The Promoter Targeting Sequence mediates epigenetically heritable transcription memory Genes & Dev., November 1, 2004; 18(21): 2639 - 2651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Estrada, F. Casares, A. Busturia, and E. Sanchez-Herrero Genetic and molecular characterization of a novel iab-8 regulatory domain in the Abdominal-B gene of Drosophila melanogaster Development, March 13, 2003; 129(22): 5195 - 5204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hogga and F. Karch Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing Development, January 11, 2002; 129(21): 4915 - 4922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Bender and D. P. Fitzgerald Transcription activates repressed domains in the Drosophila bithorax complex Development, January 11, 2002; 129(21): 4923 - 4930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Fitzgerald and W. Bender Polycomb Group Repression Reduces DNA Accessibility Mol. Cell. Biol., October 1, 2001; 21(19): 6585 - 6597. [Abstract] [Full Text] [PDF]
|
|
